Product Catalogue. Specialist Materials for Research. Antibodies. Antigens. Assay Components. Specialist Immunologicals
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1 Product Catalogue Specialist Materials for Research Antibodies s Assay Components Specialist Immunologicals
2 About Binding Site Binding Site is a Specialist Protein company committed to the research, development, manufacture and distribution of innovative immunodiagnostic assays and immunological materials for the global market. With extensive expertise in antibody specificity technology, Binding Site gives clinicians, manufacturers, researchers and laboratory staff the tools to significantly improve diagnosis and management of patients across a range of conditions. Introduction to Immunologicals from Binding Site As a manufacturer of a wide range of innovative in vitro diagnostic products, Binding Site understands the necessity for high quality raw materials and components. With a proven track record of over 30 years, our expertise in immunisation and processing techniques has led to our international recognition as a key supplier of antibodies, antigens, bulk antisera, clinical specimens and diagnostic reagents. Our flexibility of scale from small R&D quantities to larger lots allow us to support your project whatever its size. Key elements for success lie within the way we work and the quality systems that are in place. Our commitment to continuous improvement allows us to enhance the quality of our product and service offering Working closely with manufacturers to ensure a focused and timely delivery of your product Provision of specialised proteins to facilitate effective research Whatever your requirements, we would welcome hearing from you and we look forward to working with you now and in the future. Quality Assurance Our manufacturing site has quality systems approved to both ISO9001 and ISO13485:2003 certification. These, together with strict adherence to rigorous internal quality control procedures, ensure customers can be confident in the quality of Binding Site products. TABLE OF CONTENTS PAGE INTRODUCTION 1 ANTIBODIES 2 Sheep Anti-Human Polyclonal Antibodies - Unconjugated 2 Sheep Anti-Human FITC Conjugated Polyclonal Antibodies 4 Sheep Anti-Human HRP Conjugated Polyclonal Antibodies 5 Monoclonal Antibodies - Anti-Human 5 Polyclonal Antibodies to Animal Proteins 6 FITC Conjugated Polyclonal Antibodies to Animal Proteins 6 HRP Conjugated Polyclonal Antibodies to Animal Proteins 6 ANTIGENS 7 Human Purified Myeloma s 7 Other Human Native s 7 ASSAY COMPONENTS 8 Bulk Volume Antibodies to Special Proteins 8 Human Rheumatoid Factor (RF)/IgG Absorbent 9 1
3 Antibodies Binding Site offers a comprehensive range of antibodies focusing on human serum proteins including their subclasses. Affinity purified, IgG fraction and conjugations to Fluorescein- Isothiocyanate (FITC) and Horseradish Peroxidase (HRP) are routinely available. Additional custom formats can be made available on request. Affinity purified antibodies (product codes starting A) are purified against an immobilised antigen. In some instances, solid phase absorption is also utilised to reduce inter-species cross reactivity. IgG fraction antibodies (product codes starting P) are IgG fractions of sheep sera. Sheep Anti-Human Polyclonal Antibodies - Unconjugated Antibodies Albumin IgG Fraction 1mL PC032 Albumin IgG Fraction 15mL PC032.X α2 HS Glycoprotein IgG Fraction 15mL PC124.X α Trypsin Inhibitor IgG Fraction 15mL PC060.X Antithrombin III IgG Fraction 1mL PC040 Antithrombin III IgG Fraction 15mL PC040.X ß2 Microglobulin IgG Fraction 15mL PC043.X C1q IgG Fraction 1mL PC020 C1q IgG Fraction 15mL PC020.X C3c IgG Fraction 15mL PC023.X C3d IgG Fraction 15mL PC024.X C4 IgG Fraction 15mL PC025.X C4 Binding Protein IgG Fraction 1mL PC026 C4 Binding Protein IgG Fraction 15mL PC026.X C5 IgG Fraction 15mL PC027.X C8 IgG Fraction 15mL PC089.X Carbonic Anhydrase 2 IgG Fraction 15mL PC076.X Elastase IgG Fraction 15mL PC052.X Factor H IgG Fraction 1mL PC030 Factor H IgG Fraction 15mL PC030.X Factor I IgG Fraction 1mL PC031 Factor I IgG Fraction 15mL PC031.X Fibrinogen IgG Fraction 15mL PC056.X Fibronectin IgG Fraction 15mL PC057.X Haptoglobulin IgG Fraction 1mL PC058 Haptoglobulin IgG Fraction 15mL PC058.X IgA IFE Grade Antisera 100mL FX010 IgA Affinity Purified 15mg AU010.X IgA IgG Fraction 1mL PC010 IgA IgG Fraction 15mL PC010.X 2
4 Sheep Anti-Human Polyclonal Antibodies - Unconjugated (continued) Antibodies 3 IgE IFE Grade Antisera 15mL FX014 IgE IgG Fraction 15mL PC014.X IgG (Fc) IFE Grade Antisera 100mL FX004 IgG (Fc) Affinity Purified 2mg AU004 IgG (Fc) Affinity Purified 15mg AU004.X IgG (Fc) [Monkey Adsorbed] Affinity Purified 1mL AU004.M IgG (Fc) [Monkey Adsorbed] Affinity Purified 15mL AU004.M.X IgG (Fc) IgG Fraction 1mL PC004 IgG (Fc) IgG Fraction 15mL PC004.X IgG (H & L) IgG Fraction 1mL PC003 IgG (H & L) IgG Fraction 15mL PC003.X IgG (H & L) [Monkey Adsorbed] Affinity Purified 1mg AU003.M IgG (H & L) [Monkey Adsorbed] Affinity Purified 15mg AU003.M.X IgG (Fd) IgG Fraction 1mL PC075 IgG (Fd) IgG Fraction 15mL PC075.X IgG1 Affinity Purified 0.5mg AU006 IgG1 Affinity Purified 15mg AU006.X IgG2 Affinity Purified 0.5mg AU007 IgG2 Affinity Purified 15mg AU007.X IgG3 Affinity Purified 0.5mg AU008 IgG3 Affinity Purified 15mg AU008.X IgG4 Affinity Purified 0.5mg AU009 IgG4 Affinity Purified 15mg AU009.X IgM IFE Grade Antisera 50mL FX012 IgM IgG Fraction 15mL PC012.X Kappa Light Chain Total Affinity Purified 2mg AU015 Kappa Light Chain Total Affinity Purified 15mg AU015.X Kappa Light Chain Total IFE Grade Antisera 50mL FX015 Lambda Light Chain Total Affinity Purified 2mg AU017 Lambda Light Chain Total Affinity Purified 15mg AU017.X Lambda Light Chain Total IFE Grade Antisera 50mL FX017 LD Isoenzyme V IgG Fraction 1mL PC154 LD Isoenzyme V IgG Fraction 15mL PC154.X Plasminogen IgG Fraction 15mL PC065.X Prealbumin IgG Fraction 15mL PC066.X Prothrombin IgG Fraction 15mL PC067.X Superoxide Dimutase (Cu/Zn) IgG Fraction 1mL PC077 Superoxide Dimutase (Cu/Zn) IgG Fraction 15mL PC077.X Superoxide Dismutase (Mn) IgG Fraction 15mL PC096.X Tamm Horsfall Protein (Uromucoid) IgG Fraction 1mL PC071 Tamm Horsfall Protein (Uromucoid) IgG Fraction 15mL PC071.X Transferrin IgG Fraction 1mL PC070 Transferrin IgG Fraction 15mL PC070.X von Willebrand Factor IgG Fraction 1mL PC054 von Willebrand Factor IgG Fraction 15mL PC054.X
5 Sheep Anti-Human FITC Conjugated Polyclonal Antibodies MINIMUM PURCHASE CONJUGATE C1q IgG Fraction 1mL FITC PF020 C1q IgG Fraction 15mL FITC PF020.X C3c IgG Fraction 1mL FITC PF023 C3c IgG Fraction 15mL FITC PF023.X C4 IgG Fraction 15mL FITC PF025.X Fibrinogen IgG Fraction 15mL FITC PF056.X IgA IgG Fraction 15mL FITC PF010.X IgA [Monkey Adsorbed] Affinity Purified 15mg FITC AF010.M.X IgG (Fc) Affinity Purified 15mL FITC AF004.X IgG [Monkey Adsorbed] Affinity Purified 15mg FITC AF004.M.X IgG1 Affinity Purified 0.5mg FITC AF006 IgG1 Affinity Purified 10mg FITC AF006.X IgGAM (H+L) IgG Fraction 15mL FITC PF002.X IgG (fc) IgG Fraction 15mL FITC PF004.X IgG2 Affinity Purified 0.5mg FITC AF007 IgG2 Affinity Purified 10mg FITC AF007.X IgG3 Affinity Purified 0.5mg FITC AF008 IgG3 Affinity Purified 10mg FITC AF008.X IgG4 Affinity Purified 0.5mg FITC AF009 IgG4 Affinity Purified 10mg FITC AF009.X IgM Affinity Purified 15mg FITC AF012.X IgM IgG Fraction 15mL FITC PF012.X IgG (H+L) Affinity Purified 15mg FITC AF003.X IgG (H+L) [Monkey Adsorbed] Affinity Purified 15mg FITC AF003.M.X Kappa Light Chain Total Affinity Purified 1mg FITC AF015 Kappa Light Chain Total Affinity Purified 15mg FITC AF015.X Lambda Light Chain Total Affinity Purified 1mg FITC AF017 Lambda Light Chain Total Affinity Purified 15mg FITC AF017.X Von Willebrand Factor IgG Fraction 1mL FITC PF054 Von Willebrand Factor IgG Fraction 15mL FITC PF054.X Antibodies Typical product data for conjugated IgG antibodies. Individual product datasheets are available on 4
6 Sheep Anti-Human HRP Conjugated Polyclonal Antibodies Antibodies CONJUGATE Albumin IgG Fraction 1mL HRP PP032 Albumin IgG Fraction 15mL HRP PP032.X α1 Antitrypsin IgG Fraction 1mL HRP PP034 α1 Antitrypsin IgG Fraction 15mL HRP PP034.X Apolipoprotein B IgG Fraction 15mL HRP PP086.X IgG (H+L) [Monkey Adsorbed] Affinity Purified 1mg HRP AP003.M IgG (H+L) [Monkey Adsorbed] Affinity Purified 15mL HRP AP003.M.X IgG (Fc) Affinity Purified 1mg HRP AP004 IgG (Fc) [Monkey Adsorbed] Affinity Purified 15mL HRP AP004.M IgG (Fc) [Monkey Adsorbed] Affinity Purified 15mL HRP AP004.M.X IgG (Fc) Affinity Purified 15mg HRP AP004.X IgG1 Affinity Purified 0.5mg HRP AP006 IgG1 Affinity Purified 10mg HRP AP006.X IgG2 Affinity Purified 0.5mg HRP AP007 IgG2 Affinity Purified 10mg HRP AP007.X IgG3 Affinity Purified 0.5mg HRP AP008 IgG3 Affinity Purified 10mg HRP AP008.X IgG4 Affinity Purified 0.5mg HRP AP009 IgG4 Affinity Purified 10mg HRP AP009.X IgA Affinity Purified 1mg HRP AP010 IgA Affinity Purified 15mg HRP AP010.X IgM Affinity Purified 1mg HRP AP012 IgM Affinity Purified 15mg HRP AP012.X Kappa Light Chain Total Affinity Purified 1mg HRP AP015 Kappa Light Chain Total Affinity Purified 15mg HRP AP015.X Lambda Light Chain Total Affinity Purified 1mg HRP AP017 Lambda Light Chain Total Affinity Purified 15mg HRP AP017.X Transferrin IgG Fraction 1mL HRP PP070 Transferrin IgG Fraction 15mL HRP PP070.X Anti-Human Monoclonal Antibodies CD23 Clone BU38 IgG Fraction 0.2mg MC112 CD23 Clone BU38 IgG Fraction Customer Specified MC112.X IgG4 MAb Clone HP6025 IgG Fraction 0.2mg MC011 IgG4 MAb Clone HP6025 IgG Fraction Customer Specified MC011.X IgG4 clone HP6025 IgG Fraction - HRP 0.2mg MP011 IgG4 clone HP6025 IgG Fraction - HRP Customer Specified MP011.X 5
7 Polyclonal Antibodies to Animal Proteins Sheep Anti-Dog IgG (H&L) IgG Fraction 15ml PC230.X Sheep Anti-Mouse IgG (H&L) Affinity Purified 15mg AU271.X Sheep Anti-Mouse a1 Hydroxylase IgG Fraction 1mL PC290 Sheep Anti-Mouse a1 Hydroxylase IgG Fraction 15ml PC290.X Sheep Anti-Rabbit IgG (H&L) Affinity Purified Customer Specified AU311.X Donkey Anti-Sheep/Goat IgG (H+L) Donkey Anti-Sheep/Goat IgG (H+L) Affinity Purified 2mg AU360 Affinity Purified Customer Specified AU360.X FITC Conjugated Polyclonal Antibodies to Animal Proteins Sheep Anti-Cat IgG (H+L) IgG Fraction 1mL PF210 Sheep Anti-Cat IgG (H+L) IgG Fraction 15ml PF210.X Sheep Anti-Dog IgG (H+L) IgG Fraction 1mL PF230 Sheep Anti-Dog IgG (H+L) IgG Fraction 15ml PF230.X Sheep Anti-Rabbit IgG (H+L) Affinity Purified 15mg AF311.X Antibodies HRP Conjugated Polyclonal Antibodies to Animal Proteins Sheep Anti-Mouse IgG (H+L) Affinity Purified 1mg AP271 Sheep Anti-Mouse IgG (H+L) Affinity Purified 15mg AP271.X Sheep Anti-Mouse IgG Affinity Purified 1mg AP272 Sheep Anti-Mouse IgG Affinity Purified 15mg AP272.X Sheep Anti-Rabbit IgG (H+L) Affinity Purified 1mg AP311 Sheep Anti-Rabbit IgG (H+L) Affinity Purified 15mg AP311.X Donkey Anti-Sheep/Goat IgG (H+L) Donkey Anti-Sheep/Goat IgG (H+L) Affinity Purified 1mg AP360 Affinity Purified 15mg AP360.X 6
8 s s Purified Human Myeloma s Binding Site myeloma antigens are isolated from human serum and extensively purified by ion exchange, gel filtration and affinity chromatography. The isotype and light chain type of the myeloma protein is determined by immunoelectrophoresis. These products are supplied in buffer at greater than 95% purity. Human IgG1 Kappa 1mL BP078 Human IgG1 Kappa 10mg BP078.X Human IgG1 Lambda 10mg BP079.X Human IgG2 Kappa 1ml BP080 Human IgG2 Kappa 10mg BP080.X Human IgG3 Kappa 1mL BP082 Human IgG3 Kappa 10mg BP082.X Human IgG3 Lambda 10mg BP083.X Human IgG4 Kappa 1mL BP084 Human IgG4 Kappa 10mg BP084.X Human IgG4 Lambda 10mg BP085.X Human IgA1 Kappa 10mg BP086.X Other Purified Human Proteins The purified antigens listed below have been isolated from human material. They are supplied in buffer at variable concentrations. Human CRP 99% Pure (Ascitic fluid) 10mg BP300.X Human CRP 80% Pure (Ascitic fluid) 10mg BP301.X Human HBsAg type ay 10mg BP302.X Human HBsAg type ad 10mg BP303.X Human Prothrombin 10mg BP314.X Human Prothrombin - Glycerol Free 10mg BP315.X Human Sex Hormone Binding Globulin 10mg BP316.X New antigens are being added regularly. Visit for our latest products. 7
9 Assay Components Human Rheumatoid Factor (RF)/IgG Absorbent The accurate quantification of human IgM in infectious disease assays may be disrupted by the presence of IgM class Rheumatoid Factor (RF) and by Immunoglobulin G (IgG). Human RF/IgG Absorbent is a reagent designed to remove both IgM class Rheumatoid Factor and IgG from serum prior to the quantification of specific IgM. Specific IgG False Negative Result Specific IgM Specific IgG False Positive Result IgM Class RF Detection Anti-Human IgM Detector Conjugated Secondary Antibody Assay Components High levels of IgG antibodies mask the lower levels of IgM antibodies which are unable to be detected. Correct Result The IgM class RF inherently binds to antigen IgG immune complexes. This leads to an erroneous test reading as a result of the secondary detector conjugated antibody. Detection Specific IgM Anti-Human IgM Detector Conjugated Secondary Antibody specific IgM in the patient sample now easily binds to immobilised antigen as intended and is detected by an IgG class-specific secondary antibody tagged with an indicator molecule capable of being read and measured. Both FALSE POSITIVE and FALSE NEGATIVE test results due to IgM Class RF and IgG are now eliminated. IgG REMOVAL CAPACITY RF / IgG Absorbent 15 mg/ml Whole Serum 1 x 10mL (Non-US) IC050 RF / IgG Absorbent 15 mg/ml Whole Serum 1 x 10mL (US) IC050.A In addition to the standard grade we are able to change the composition & characteristics of this product to suit different applications such as ELISA or liquid phase assay. In collaboration with manufacturers of infectious disease assays, we have developed customised variants of this product that are optimised for either high titre IgG removal or limited cross reactions to non-target infectious agents. 8
10 Assay Components We can provide IVD manufacturers and researchers with products offering the highest degrees of performance, purity, shelf life stability, and lot-to-lot consistency. Additionally, as our reagent manufacturing facility operates to both ISO9001 and ISO13485:2003 quality systems, you can be reassured that your product will be made to the required specifications and documented accordingly. Reagent Types Potential Applications Menu SPECIAL PROTEINS SECONDARY Abs INFECTIOUS DISEASES Neph / Turb / ELISA W/B / IHC / ELISA IgG, IgA, IgM, Albumin, Pre-Albumin C3, CRP HRP / FITC conjugated Abs to Human / Animal Antibody Pairs ELISA / Lateral Flow HbsAg, HBV, HDV, HIVp24 s (Recombinant) Multiple COMING SOON HDV, HIVp24, HBe, HBc, Chagas RF / IgG Removal ELISA Up to 15mg/mL IgG removal can be customised to optimise performance in different techniques Please contact us for specific details of your requirements. 9
11 visit us for more information
12 Head Office: The Binding Site Group Ltd 8 Calthorpe Road Edgbaston Birmingham B15 1QT Tel: Fax: immuno@bindingsite.co.uk Germany, Austria, Switzerland: The Binding Site GmbH Robert-Bosch-Str. 2A Schwetzingen Germany Tel: +49 (0) Fax: +49 (0) office@bindingsite.de USA, Canada, Latin America: The Binding Site Inc Mesa Ridge Road San Diego CA United States of America Tel: Fax: Toll Free: info@thebindingsite.com Italy: The Binding Site Italy Via Zanica Grassobbio (BG) Italy Tel: Fax: immuno@bindingsite.co.uk immuno@bindingsite.co.uk 2016, The Binding Site Group Ltd, Birmingham, UK. September 2016 MKG883.E
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