DNA Structure and Function
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1 DNA Structure and Function DNA Structure DNA Replication Ch 4: Nucleic Acids and the Origin of LIfe Ch 13: DNA and its role in heredity
2 Discussion Summary: Week 12 Stirring the Simmering Designer Baby Pot Team Discussion: Questions Take home message Group discussion Take home messages - one from each group Questions- one from each group
3 Nucleic Acid Structure ff Purine / Pyrimidine Base pair Ribose Phosphate Nucleotide Double-helix Access Excellence Michael Blaber
4 Nucleic Acid Structure ff Access Excellence Michael Blaber DNA Structure Purine / Pyrimidine Base pair
5 Nucleic Acid Structure Ribose Deoxyribose Michael Blaber h8p:// DNA Structure
6 Nucleic Acid Structure Phosphate Nucleotide Michael Blaber DNA Structure
7 Nucleic Acid Structure Nucleotide polymer Michael Blaber DNA Structure
8 DNA Structure B form of DNA Anti-parallel
9 Biochemistry Chargaff Modeling Crick and Watson Structural Biology Rosalind Franklin DNA Structure and Function Multidisciplinary Endeavor
10 Central Dogma DNA à RNA à Protein DNA Function ERA Net genome.aspx
11 DNA Function Gene Structure Promoter 5 UTR Coding region 3 UTR Terminator University of Arizona
12 DNA and Heredity! Classic experiments
13 DNA as Genetic Material Classic Experiments Griffith s Transformation Streptococcus pneumoniae How did the mouse die when the only living strain was not virulent? DNA and heredity U of Cincinnati Claremont: Dr. Frankhauser
14 DNA as Genetic Material Classic Experiments Avery, MacLeod, McCarty Which fraction of the virulent cell can transform the non virulent strain? Vision Learning.com DNA and heredity
15 DNA as Genetic Material Classic Experiments Hershey and Chase E. coli Which molecule from a phage moves to the bacterial cell? Access Excellence DNA and heredity
16 Investigating Life : Terminology Transformation Permanent DNA change to Bacterial and non-animal Eukaryotic cells Transfection Impermanent DNA change Sometimes means any added DNA in animals Transduction Chapter 13 Using a virus vector DNA and heredity h8p:// thread-1775.html
17 DNA Replication Steps Break H-bond New strand RNA primer Players Helicase DNA polymerase Template DNA Nucleotides (dntp s) Result 2 DNA double-helices, identical copies of one Extra details Methylation of new DNA DNA repair Mutations 5 to 3 (3 to 5 possible, but does not happen)
18 DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination What when how.com Contexto Info
19 Semiconservative Replication Experiment Meselson-Stahl Grow cells in heavy nitrogen source ( 15 N) Density-gradient centrifugation of DNA. Let cells replicate in normal nitrogen source ( 15 N) Density-gradient centrifugation of DNA Repeat for another generation Steven Carr DNA Replication
20 DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination Supercoling and replication: Stone et al DNA Replication
21 DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination Wikipedia Dolan DNA learning Center
22 DNA Replication Overview Circular DNA Important proteins Replication fork Leading/lagging strands Termination: DNA gyrase splits Nobel Overviews: medicine/dna/a/replication/ replication_ani.html dna/a/replication/lagging_ani.html Wikipedia
23 Some great DNA animations Chromosomes are always there just not easy to see replication1.swf Harvard is always accurate =) So is Cold Spring Harbor! Don t forget Howard Hughes Medical Institute (HHMI)
24 STRETCH BREAK!
25 STRETCH BREAK!
26 This week s lab: Lab 5a Intro Punnett squares Meiosis generates a diversity haploids via crossing-over, independent assortment Fertilization generates diversity because it is random Punnett squares help us figure out all possible combinations Real Data Track real phenotypes in progeny from known crosses Compare with predictions Molecular genotyping Genotypes can be inferred through phenotypes and pedigrees Genotypes can be determined directly through molecular means (PCR, restriction digests, sequencing)
27 Next week s lab: Lab 5b week 1 intro Culture Pseudomonas syringae (2 strains) for innoculations Prepare dilutions of bacterial solutions for controlled experiment Innoculate A. thaliana with pathogen Predict phenotypes based on genotypes and classical genetic analysis from lab 5a
Transformation: change in genotype & phenotype due to assimilation of external DNA by a cell.
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