Brian E. Applegate, Ph.D.

Transcription

1 Education and Training Brian E. Applegate, Ph.D. B.S. in Chemistry, June 1994 Wright State University, Dayton, OH cum laude M.S. in Chemistry, June 1998 Ohio State University, Columbus, OH Thesis: The Spectroscopy and Dynamics of Transient Radicals: The Perdeuteromethoxy radical Advisor: Terry A. Miller Ph.D. in Chemistry, December 2000 Ohio State University, Columbus, OH Dissertation: Experimental and Theoretical study of the Jahn-Teller Effect in the Cyclopentadienyl Radical and the Benzene Family of Cations Advisor: Terry A. Miller Postdoctoral Fellow, August 2000 August 2003 University of North Carolina at Chapel Hill Department of Chemistry Advisor: Roger E. Miller Postdoctoral Fellow, September 2003-August 2006 Duke University Department of Biomedical Engineering Advisor: Joseph A. Izatt Professional Experience 9/06-present Assistant professor, Dept. of Biomedical Engineering, Texas A&M University, College Station, TX 9/05-6/06: Consultant, Bioptigen Inc., Durham, NC 9/03-9/06 Postdoctoral Fellow, Dept. of Biomedical Engineering, Duke University, Durham, NC 8/00-8/03 Postdoctoral Fellow, Dept. of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC Honors and Awards 2004 NIH NRSA individual postdoctoral fellowship award 2011 NSF CAREER award List of Publications (reverse chronological order) Book Chapters 1. A. Oldenburg, B. E. Applegate, J. A. Izatt, S. A. Boppart, Molecular OCT Contrast Enhancement and Imaging, In Optical Coherence Tomography: Technology and Applications, J. Fujimoto, W. Drexler, Eds. (Springer: New York, NY, USA, 2008). 2. B. E. Applegate, and J. A. Izatt, "Molecular Contrast OCT," in Optical Coherence Tomography in Cardiovascular Research, E. Regar, A. M. G. J. v. Leeuwen, and P. W. Serruys, eds. (Informa Healthcare, 2007). Journal Articles (peer reviewed since 2004) 1. J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, and J. A. Jo, Biochemical characterization of atherosclerotic plaques by endogenous multispectral fluorescence lifetime imaging, Atherosclerosis, accepted Brian E. Applegate 1

2 2. B. E. Applegate, R. L. Shelton, S. Gao, J. S. Oghalai, Imaging high frequency periodic motion in the mouse ear with coherently interleaved Optical Coherence Tomography, Optics Letters, accepted 3. S. Gao, A. Xia, T. Yuan, P. Raphael, R. L. Shelton, B. E. Applegate, and J. S. Oghalai, Quantitative imaging of cochlear soft tissues in wild-type and hearing-impaired transgenic mice by spectral domain optical coherence tomography, Opt. Express 19, (2011) 4. Q. Wu, B. E. Applegate, and A. T. Yeh, Cornea microstructure and mechanical response measured with nonlinear optical and optical coherence microscopy using sub-10-fs pulses, Biomed. Opt. Express 2, (2011). 5. R. L. Shelton and B. E. Applegate, Ultrahigh Resolution Photoacoustic Microscopy via Transient Absorption, Biomed. Opt. Express 1, (2010). 6. J. A. Jo, B. E. Applegate, J. Park, S. Shrestha, P. Pande, I. B. Gimenez-Conti, and J. L. Brandon, "In-Vivo Simultaneous Morphological and Biochemical Optical Imaging of Oral Epithelial Cancer," IEEE Trans Biomed Eng 57, (2010). 7. J. Park, J. A. Jo, S. Shrestha, P. Pande, Q. Wan, and B. E. Applegate, "A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization," Biomed. Opt. Express 1, (2010). 8. S. Shrestha, B. E. Applegate, J. Park, X. Xiao, P. Pande, and J. A. Jo, "High-Speed Multispectral Fluorescence Lifetime Imaging Implementation for in vivo applications," Optics Letters 35, (2010). 9. D. Jacob, R. L. Shelton, and B. E. Applegate, "Fourier domain pump-probe optical coherence tomography imaging of Melanin," Optics Express 18, (2010). 10. R. L. Shelton and B. E. Applegate, "Off-Axis Photoacoustic Microscopy," IEEE Trans Biomed Eng 57, (2010). 11. Q. Wan, and B. E. Applegate, "Multiphoton coherence domain molecular imaging with pump-probe optical coherence microscopy," Opt. Lett. 35, (2010). 12. E. J. McDowell, A. K. Ellerbee, M. A. Choma, B. E. Applegate, and J. A. Izatt, "Spectral domain phase microscopy for local measurements of cytoskeletal rheology in single cells," Journal of Biomedical Optics 12, (2007). 13. B. E. Applegate and J. A. Izatt, "Molecular imaging of endogenous and exogenous molecular chromophores with ground state recovery pump-probe optical coherence tomography," Optics Express 14, (2006). 14. M. V. Sarunic, B. E. Applegate, and J. A. Izatt, "Real-time quadrature projection complex conjugate resolved Fourier domain optical coherence tomography," Optics Letters 31, (2006). 15. B. E. Applegate, C. Yang, and J. A. Izatt, "Theoretical comparison of the sensitivity of molecular contrast optical coherence tomography techniques," Optics Express 13, (2005). 16. M. V. Sarunic, B. E. Applegate, and J. A. Izatt, "Spectral domain second harmonic optical coherence tomography," Optics Letters 30, (2005). 17. B. E. Applegate, C. Yang, A. M. Rollins, and J. A. Izatt, "Polarization resolved second harmonic generation optical coherence tomography in collagen," Optics Letters 29, (2004). 18. W. K. Lewis, B. E. Applegate, J. Sztaray, B. Sztaray, T. Baer, R. J. Bemish, and R. E. Miller, "Electron impact ionization in helium nanodroplets: Controlling fragmentation by active cooling of molecular ions," J Am Chem Soc 126, (2004). 19. C. Yang, L. E. L. McGuckin, J. D. Simon, M. A. Choma, B. E. Applegate, and J. A. Izatt, "Spectral triangulation molecular contrast optical coherence tomography with indocyanine green as the contrast agent," Optics Letters 29, (2004). List of Presentations (reverse chronological order) Invited 1. B. E. Applegate, Simultaneous High-Resolution Morphological and Biochemical Optical Imaging of Atherosclerosis, Atherosclerosis and Vascular Biology Training Center, Baylor College of Medicine, June 23, 2011, Houston, TX 2. R. L. Shelton and B. E. Applegate, Development of Transient Absorption Ultrasonic Microscopy, European Conference on Biomedical Optics (SPIE, OSA), May 24, 2011, Munich, Germany. Brian E. Applegate 2

3 3. B. E. Applegate, Simultaneous Morphological and Biochemical Imaging for Cancer Diagnosis and Atherosclerotic Plaque Discrimination, Optics in the Life Sciences (OSA), April 4, 2011, Monterey, CA. Contributed (w/ proceedings since 2009) 1. O. Carrasco-Zevallos, R. L. Shelton, C. Olsovsky, M. Saldua, B. E. Applegate, and K. C. Maitland, Exploiting chromatic aberration to spectrally encode depth in reflectance confocal microscopy, Proc. SPIE 8086 (2011) 2. J. S. Oghalai, A. Xia, C. C. Liu, S. Gao, B. E. Applegate, S. Puria, I. Rousso, and C. Steele, Biophysical mechanisms underlying hearing loss associated with shortened tectorial membrane, Mechanics of Hearing (2011). 3. S. Gao, A. Xia, T. Yao, P. Raphael, R. L. Shelton, B. E. Applegate, J. S. Oghalai, Imaging of the intact mouse cochlea by spectral domain optical coherence tomography. in Proc. SPIE (2011). 4. R. L. Shelton and B. E. Applegate, "Development of Transient Absorption Ultrasonic Microscopy," in Proc. SPIE (2011). 5. P. Pande, S. Shrestha, J. Park, B. E. Applegate, and J. Jo, In vivo detection of oral cancer based on OCT-derived morphological and FLIM derived biochemical biomarkers. in Proc. SPIE (2011). 6. J. Park, P. Pande, S. Shrestha, B. E. Applegate, and J. Jo, Simultaneous coregistered morphological and biochemical imaging of atherosclerotic plaques. In Proc. SPIE (2011). 7. S. Shrestha, B. E. Applegate, P. Pande, and J. Jo, "Design of a dual-modality imaging system using optical coherence tomography and fluorescence lifetime imaging for oral cancer detection," in Proc. SPIE (2010). 8. S. Shrestha, B. E. Applegate, P. Pande, and J. Jo, "Multimodality optical imaging combining optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) for morphological and biochemical tissue characterization," in Proc. SPIE (2010). Contributed (w/o proceedings since 2009) 1. P. Pande, S. Shrestha, J. Park, B. Applegate, J.A. Jo, In Vivo Detection of Oral Cancer Based on OCT-Derived Morphological and FLIM-Derived Biochemical Features of the Oral Mucosa, SBEC, Dallas, TX (2011) 2. J. Park, P. Pande, S. Shrestha, B.E. Applegate, F. Clubb, J.A. Jo, Multimodal Optical Imaging for Simultaneous High-Resolution Morphological and Biochemical Characterization of Coronary Atherosclerotic Plaque, SBEC, Dallas, TX (2011) 3. K. C. Maitland, O. Carrasco-Zevallos, R. L. Shelton, C. Olsovsky, M. Saldua, and B. E. Applegate, Exploiting chromatic aberration to spectrally encode depth in reflectance confocal microscopy, European Conference on Biomedical Optics, Munich, Germany (2011) 4. R. L. Shelton and B. E. Applegate, Development of Transient Absorption Ultrasonic Microscopy, CLEO, Baltimore, Md (2011) 5. B. E. Applegate, J. Park, S. Shrestha, P. Pande, I. B. Gimenez-Conti, J. L. Brandon, and J. Jo, Simultaneous Morphological and Biochemical Imaging of Oral Epithelial Cancer in a Hamster Cheek Pouch Model, CLEO, Baltimore, Md (2011) 6. J. Jo, J. Park, P. Pande, S. Shrestha, F. Clubb, B. E. Applegate, Simultaneous High-Resolution Morphological and Biochemical Optical Imaging of Atherosclerosis, Arteriosclerosis, Thrombosis, and Vascular Biology, Chicago, Il (2011) 7. B. E. Applegate, Q. Wan, and N. Warnasooriya, Development of multi-photon coherence domain molecular imaging, Optics in the Life Sciences, Monterey, CA (2011) 8. S. Shrestha, J. Park, P. Pande, B. E. Applegate, J. Jo, Multimodal Optical Coherence Tomography and Fluorescence Lifetime Imaging combined system for diagnosis of oral cancer, BMES, Austin, TX (2010) 9. J. Park, S. Shrestha, P. Pande, B. E. Applegate, J. Jo, Dual-modal optical imaging by combining OCT and FLIM for characterization of atherosclerotic plaque, BMES, Austin, TX (2010) 10. J. Park, S. Shrestha, P. Pande, J. Jo, B. E. Applegate, Design and development of an integrated OCT and FLIM catheter for percutaneous investigation of atherosclerotic plaques, BMES, Austin, TX (2010) Brian E. Applegate 3

4 11. R. L. Shelton and B. E. Applegate, "Development of Transient Absorption Ultrasonic Microscopy," BMES, Austin, TX (2010) 12. R. L. Shelton and B. E. Applegate, "Multiphoton High-Resolution Photoacoustic Microscopy," Biomed, Miami, FL (2010) 13. Q. Wan and B. E. Applegate, "Multiphoton coherence domain molecular imaging," CLEO, San Jose, CA (2010) 14. T. Yuan, R. Shelton, B. E. Applegate, S. Gao, and J. S. Oghalai, Imaging of the mouse cochlea by frequency domain optical coherence tomography Abstracts of the Midwinter Research Meeting of the Association for Research in Otolaryngology 2010, Anaheim, CA (2010) 15. J. Jo, B. E. Applegate, S. Shrestha, X. Xia, and P. Pande, Multimodality optical imaging combining Optical Coherence Tomography (OCT) and Fluorescence Lifetime Imaging (FLIM) for morphological and biochemical tissue characterization, BMES, Pittsburgh, PA (2009) 16. D. Jacob, Q. Wan, R. L. Shelton, and B.E. Applegate, Coherence Domain Molecular Imaging Based on Two-Photon Pump-Probe spectroscopy, BMES, Pittsburgh, PA (2009) 17. A. T. Yeh, Q. Wu and B. E. Applegate, Multimodal microscopy using ultrashort laser pulses for corneal mechanical depth profiling, NIH-Inter-Institute Workshop on Optical Diagnostic and Biophotonic Methods from Bench to Bedside, Bethesda, MD (2009) 18. D. Jacob, Q. Wan, R. L. Shelton, and B.E. Applegate, Coherence domain molecular imaging: pump-probe OCT and pump-probe OCM, NIH-Inter-Institute Workshop on Optical Diagnostic and Biophotonic Methods from Bench to Bedside, Bethesda, MD (2009) 19. Q. Wu, B. E. Applegate, and A. T. Yeh, Spectral domain optical coherence and nonlinear optical microscopy using sub 10-fs source, HSEMB, Houston, TX (2009) 20. X. Xiao, S. Shrestha, Q. Wan, J. Jo, and B. E. Applegate, A novel optical multimodality imaging system development for simultaneous micro-anatomical and biochemical imaging of tissue in-vivo and ex-vivo, HSEMB, Houston, TX (2009) 21. D. Jacob, R. L. Shelton, and B. E. Applegate, "Fourier Domain Pump-Probe Optical Coherence Tomography Imaging of Melanin," CLEO, Baltimore, MD (2009) 22. J. A. Jo, B. E. Applegate, C. A. Trivedi, P. Thomas, D. Jacob, R. Shelton, F. Clubb, and B. Keller, Multimodality Optical Imaging of Atherosclerotic Plaques Combining Optical Coherence Tomography and Fluorescence Lifetime Imaging, CLEO, Baltimore, MD (2009) Patents 1. J. A. Izatt, D. K. Rao, C. Yang, M. A. Choma, S. Yazdanfar, A. M. Rollins, B. E. Applegate, Method for optical coherence tomography imaging with molecular contrast. 7,075,658, July 11, 2006, USA. 2. M. V. Sarunic, B. E. Applegate, J. A. Izatt, Methods, systems, and computer program for performing real-time quadrature projection based Fourier domain optical coherence tomography. 7,903,256, March 8, 2011, USA 3. S. Freedman, P. McKinley, P. Challa, L. W. Herndon, C. A. Toth, R. Nappi, B. Dodge, B. E. Applegate, J. A. Izatt, Ophthalmic instruments, systems and methods especially adapted for conducting simultaneous tonometry and pachymetry. Pending ( ) 4. J. S. Oghalai, B. E. Applegate, S. Gao, P. Rafael, Technique for measuring and analyzing sound vibrations within the inner ear, Provisional Patent filed 9/2/2011 (61/530,806) Academic Service Member, Steering Committee, Spectroscopy Institute at The Ohio State University, 1999 Reviewer, Journal of Biomedical Optics, Journal of the Optical Society of America, Optics Express, Biomedical Optics Express, IEEE Transactions on Biomedical Engineering, Lasers in Surgery and Medicine, IEEE Journal of Selected Topics in Quantum Electronics, Optics Letters. Member, Alternate chair, CLEO 10: Medical and Biological Applications Subcommittee, for the Conference on Lasers and Electro-Optics ( ) Chair, CLEO 10: Biophotonics and Optofluidics, for the Conference on Lasers and Electro-Optics ( ) Brian E. Applegate 4

5 Member, National Advisory Committee, 16th International Conference on Luminescence (2010- present) Coordinator, Engineering Scholars Program, Dept. of Biomedical Engineering, Texas A&M University (2010-present) Member, Undergraduate Curriculum Committee, Dept. of Biomedical Engineering, Texas A&M University (2011-present) Reviewer, NIH: ETTN E12 study section, (2/28/2011), ZRG1 IMST-G mail review (11/7/2011) Professional Affiliations Optical Society of America, member (2003-present) The International Society for Optical Engineering (SPIE), member (2003-present) Society for Molecular Imaging (2009- present) Biomedical Engineering Society (2010-present) Research Grants NSF/CAREER, CBET , (PI), 6/01/2011-5/31/2016, $400,000 Development of multiphoton photoacoustic microscopy for high-resolution imaging DOD, W81XWH , (Co-PI), 11/15/ /14/2013, $3,192,936 Diagnosis and Treatment of Blast-Induced Hearing Loss NIH/NCRR, 1R21RR025799, (PI), 6/01/2009 5/31/2012, $508,704 Development of High-Resolution Molecular Imaging of Endogenous Chromophores with Pump- Probe Optical Coherence Microscopy NIH/NIBIB, 1R21EB007729, (PI), 3/01/2008 5/31/2011, $382,775 New technologies for high-resolution optical molecular imaging NIH/NCI, 1R21CA132433, (Co-PI), 5/15/2008 4/30/2011, $663,606 Combined OCT/FLIM biochemical and anatomical imaging for early cancer diagnosis NIH/NIBIB, 1F32EB004237, (PI), 9/01/2004 8/31/2006, $101,000 Bringing molecular contrast to OCT Brian E. Applegate 5