OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :31:24 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: White spot disease Address of laboratory: Maricultural Organism Disease Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, $106 Nanjing Road, Qingdao, Shandong Province266071, CHINsnA (PEOPLES REP. OF) Tel.: Fax: address: huangjie@ysfri.ac.cn Website: Name (including Title) of Head of Laboratory (Responsible Official): Cheng-Yin Shi, Senior Researcher Name (including Title and Position) of OIE Reference Expert: Jie Huang, Senior Researcher, Principal Investigator Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Nested-PCR Histopathology 60 0 In situ hybridization 20 0 T-E staining 7 0 Direct diagnostic tests Nationally Internationally TEM 31 0 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients WSSV infected tissues PCR Provide 100g 0g 1 WSSV nucleic acid PCR Provide 10^6reactions 0reactions 1 High Sensitive Detection Kits for WSSV LAMP produced 386Kits 3Kits 2 High Sensitive Detection Kits for VPahpnd LAMP produced 350Kits 7Kits 2 High Sensitive Detection Kits for DNA Platform LAMP produced 0Set 9Sets 1 High Sensitive Detection Kits for TSV LAMP produced 75Kits 0Set 1 OIE Reference Laboratory Reports Activities,

4 High Sensitive Detection Kits for EHP LAMP produced 745Kits 0Set 1 High Sensitive Detection Kits for CMNV LAMP produced 190Kits 0Kits 1 CMNV plasmid PCR produced 0ug 1ug 2 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? 4 OIE Reference Laboratory Reports Activities, 2016

5 Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) LAMP-based high sensitive detection kit for rapid use on field for white spot syndrome virus Real-time PCR assay for detecting microsporidian Enterocytozoon hepatopenaei Real-time reverse-transcription loopmediated isothermal amplification (LAMP) for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus Ma F, Zhang Q-L, Wan X-Y, Qiu L, Huang J*. Validation of the LAMP-based high sensitive detection kit for rapid use on field for white spot syndrome virus Chinese Journal of Preventive Veterinary Medicine, 2016, 38(4): Liu Z, Zhang Q-L, Wan X-Y, Ma F, Huang J*. Development of real-time PCR assay for detecting microsporidian Enterocytozoon hepatopenaei and the application in shrimp samples with different growth rates. (Chinese J.) Progress in Fishery Sciences, 2016, 37(2): Yang H-L, Qiu L, Liu Q, et al. A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus. Letters in Applied Microbiology, 2016, 63(2): ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? OIE Reference Laboratory Reports Activities,

6 Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided FRANCE Procedure for YHV-8 detection Diagnostic procedure for YHV-8 is referenced Manual of Diagnostic Tests for Aquatic Animals 2016 MALAYSIA Participated discussions and provided suggestions and reference on the cases of CMNV. Electronically by suggesting diagnostic techniques. MALAYSIA Discussion with Dr. Mohamed Shariff on AHPND According to the questions in Dr. Shariff's , answers were provide via a . EGYPT Answere questions from Dr. Mohamed Megahed on diagnostic kits for shrimp pathogens Answers were provide via Skype and WhatsApp CHINA (PEOPLE'S REP. OF) To conclude the results of target surveillance 2015 for WSSV and provide the risk analysis for WSD The advice was requested by the National Fisheries Technology Extension Centre of China (NFTEC). A chapter "Analysis on white spot disease (WSD) in 2015" was finished and pressed in "Analysis of Important Diseases for Aquatic Animals of China in 2015" by Chinese Agriculture Press. Relevant information on WSD analysis was also involved in "The National Annual Report for Aquatic Animal Health Status of China in 2015" ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 6 OIE Reference Laboratory Reports Activities, 2016

7 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Letter of Intent between Universiti Malaysia Terengganu and Yellow Sea Fisheries Research Institute Start from v 25th, 2016 Collaborative study on aquatic animal health Universiti Malaysia Terengganu MALAYSIA Dlsease Prevention and Control of Aquaculture Shrimp Start from v 11th, 2016 Collaborative study on shrimp health National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency THAILAND ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 22 1) Wang Y-J, Wang X*, Huang J, Li J. Adjuvant effect of Quillaja saponaria saponin (QSS) on protective efficacy and IgM generation in turbot (Scophthalmus maximus) upon immersion vaccination. Int. J. Mol. Sci., 2016, 17(3), 325: ) Yang H-L, Qiu L, Liu Q, et al. A novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (YHV-8) of yellow head virus. Letters in Applied Microbiology, 2016, 63(2): ) Chai P-C, Song X-L*, Chen G-F, Xu H, Huang J. Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus. Fish & Shellfish Immunology, 2016, 54: ) Zhang X-J, Song X-L*, Huang J. Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) coinfection on survival of penaeid shrimp Litopenaeus vannamei. Chinese Journal of Oceanology and Limnology, 2016, 34(6): ) Li C, Gao X-X, Huang J, Liang Y*. Studies of the viral binding proteins of shrimp BP53, a receptor of white spot syndrome virus. J Invertebr Pathol. 2016, 134: ) Wang X-F, Liu Q-H*, Wu Y, Huang J. Litopenaeus vannamei clathrin coat AP17 involved in white spot syndrome virus infection. Fish & Shellfish Immunology, 2016, 52: ) Guan G-K, Liu Q-H*, Li C, Huang J. Cloning of Litopenaeus vannamei CD63 and it's role in white spot syndrome virus infection. Developmental and Comparative Immunology. 2016, 60: ) Yan D-C*, Huang J, Yang B, Sun H-S, Wang Y-Y, Liu X. Competition of infectious hypodermal and haematopoietic necrosis virus (IHHNV) with white spot syndrome virus (WSSV) for binding to shrimp cellular membrane. Journal of Fish Diseases, 2016, 39: OIE Reference Laboratory Reports Activities,

8 9) Huang J*, Zeng L-B, Dong X, Liang Y, Xie G-S, Zhang Q-L. Trend analysis and policy recommendation on aqutic biosecurity in China. (Chinese J.) Engineering Sciences, 2016, 18(3): ) Dong X, Liang Y, Huang J*. Global biosecurity strategy and enlightenments from worldwide practice. (Chinese J.) Engineering Sciences, 2016, 18(3): ) Sun X-Y, Liu Q-H*, Wan X-Y, Huang J. Comparison of the missing sequences of ORF14/15 and ORF23/24 of WSSV from different regions of China in (Chinese J.) Progress in Fishery Sciences, 2016, 37(4): ) Wang X-F, Liu Q-H*, Wu Y, Huang J. cdna cloning of coat-epsilon gene and its tissue distribution in Fenneropenaeus chinensis. (Chinese J.) Progress in Fishery Sciences, 2016, 37(4): ) Zhu L-L, Zhang Q-L, Wan X-Y, Qiu L, Ma F, Huang J*. Molecular epidemiology of a new yellow head virus strain in China. (Chinese J.) Progress in Fishery Sciences, 2016, 37(3): ) Wang L, Wang Z-J, Gao G, Huang J*. Identification and application of a probiotic strain functioning in both ammonia nitrogen removal and Vibrio parahaemolyticus antagonism. (Chinese J.) Progress in Fishery Sciences, 2016, 37(3): ) Zbang H-H, Wang X-H*, Li C, Huang J. Isolation and identification of a bacterial strain with Vibrios-antagonism from shrimp ponds. (Chinese J.) Progress in Fishery Sciences, 2016, 37(3): ) Wang N, Wang H-L, Bai N, Huang J*, Xie D-X, Xie Q-L, Yu J-Q, Wan X-H. Isolation of lysogenic phage in Vibrio parahaemolyticus and its relationship with the pathogenicity of the host bacteria. (Chinese J.) Progress in Fishery Sciences, 2016, 37(2): ) Zhang H-H, Wang X-H*, Li C, Huang J. Isolation and identification of a Bacillus sp. strain and its role in bioflocs for the shrimp culture system. (Chinese J.) Progress in Fishery Sciences, 2016, 37(2): ) Liu Z, Zhang Q-L, Wan X-Y, Ma F, Huang J*. Development of real-time PCR assay for detecting microsporidian Enterocytozoon hepatopenaei and the application in shrimp samples with different growth rates. (Chinese J.) Progress in Fishery Sciences, 2016, 37(2): ) Sun X-Y, Wan X-Y, Liu Q-H*, Huang J. Sequence comparison of WSSV variable regions from different parts of China in (Chinese J.) Progress in Fishery Sciences, 2016, 37(2): ) Zhang X-J, Song X-L*, Wan X-Y, Yang B, Huang J. The analysis of the quantity and the composition of cultivable bacteria in artificiallly cultivated prawn larvae. (Chinese J.) Progress in Fishery Sciences, 2016, 37(1): ) Wang Q-C, Bai C-M, Zhang T-W, Wang C-M*, Qiu Z-X, Huang J. Pathogenicity of Ostreid herpesvirus-1 to Scapharca broughtonii. Journal of Fisheries of China, 2016, 40(3): ) Ma F, Zhang Q-L, Wan X-Y, Qiu L, Huang J*. Validation of the LAMP-based high sensitive detection kit for rapid use on field for white spot syndrome virus Chinese Journal of Preventive Veterinary Medicine, 2016, 38(4): b) International conferences: 4 1) Huang J. Research progress and biosecurity control strategies against EHP and AHPND for shrimp farming industry of China. FAO Second International Technical Seminar/Workshop on Acute Hepatopancreatic Necrosis Disease (AHPND); FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502. Bangkok, Thailand, on June ) Huang Jie. Shrimp epidemiology and biosecurity control. The International Workshop on Aquaculture Health, Qingdao, China, on July, ) Huang J. Shrimp epidemiology and biosecurity system. International Workshop on Aquatic Animal Health in Shenzhen AQSIQ. Shenzhen, Guangdong, v 8, ) Huang J. Status of IMNV in China and actions taken by the government, private sector and the academe. FAO/NFTEC/YSFRI Workshop on Emergency Preparedness and Response and Contingency Planning, FAO TCP/INT/3501: Strengthening biosecurity governance and capacities for dealing with the serious shrimp infectious myonecrosis virus (IMNV) disease. Beijing, China, on vember, c) National conferences: 3 1) Huang J. Shrimp epidemiology and biosecurity control system. Workshop for Marine Economic Development of Beihai, Beihai, Guangxi, Jan 7, ) Huang J. Microbiological control technology for shrimp diseases. The Sixth Conference on Strategies of Leading Industry Development of Probiotics, Beihai, Guangxi, May 14, ) Huang J. Shrimp epidemiology and biosecurity control system. Workshop on Blue Economic Development of Dongying, Kengli, Shandong, Aug 20, d) Other: (Provide website address or link to appropriate information) 7 1) Dong X, Huang J. Analysis on infectious hypodermal and hematopoietic necrosis in In: Bureau of Fisheries, MOA; National Fisheries Technology Extension Center of China. eds. Analysis on Important Diseases of Aquatic Animals in China in 2015, 2016, China Agriculture Press, Beijing, p ) Dong X, Huang J. Analysis on white spot disease (WSD) in In: Bureau of Fisheries, MOA; National 8 OIE Reference Laboratory Reports Activities, 2016

9 Fisheries Technology Extension Center of China. eds. Analysis on Important Diseases of Aquatic Animals in China in 2015, 2016, China Agriculture Press, Beijing, p ) Dong X, Huang J. Analysis on white spot disease (WSD). In: Bureau of Fisheries, MOA; National Fisheries Technology Extension Center of China. eds. Analysis on Important Diseases of Aquatic Animals in China in 2014, 2016, Marine Press, Beijing, ) Wan X-Y, Bai C-M, Shi C-Y, Yang B, Huang J. Quarterly Aquatic Animal Disease Report of the Maricultural Organism Disease Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences to the Ministry of Agriculture of China, the first quarter of ) Wan X-Y, Bai C-M, Shi C-Y, Yang B, Huang J. Quarterly Aquatic Animal Disease Report of the Maricultural Organism Disease Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences to the Ministry of Agriculture of China, the second quarter of ) Wan X-Y, Bai C-M, Shi C-Y, Yang B, Huang J. Quarterly Aquatic Animal Disease Report of the Maricultural Organism Disease Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences to the Ministry of Agriculture of China, the third quarter of ) Wan X-Y, Bai C-M, Shi C-Y, Yang B, Huang J. Quarterly Aquatic Animal Disease Report of the Maricultural Organism Disease Control and Molecular Pathology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences to the Ministry of Agriculture of China, the fourth quarter of ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 6 b) Seminars: 2 c) Hands-on training courses: 1 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Cambodia, Sri Lanka, Thailand, Iraq, Vietnam 28 participants a Egypt 1 participant a Indonesia 1 participant a USA, Canada, Vietnam, Brunei, Myanmar 6 participants a Malaysia 7 participants a rway 3 participants b USA, Canada, Vietnam, Brunei, Myanmar, Cambodia, Sri Lanka, Thailand, Iraq, Vietnam 34 participants b FAO, Thailand, Indonesia, Mexico, Brazil 10 participants c Cambodia, Sri Lanka, Thailand, Iraq, Vietnam 28 participants OIE Reference Laboratory Reports Activities,

10 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Explain Quality Management System in adoption process or currently in place The application for the official certification is expected to be submitted before All diagnostic activities and QMS for the OIE laboratory was operated according to the requirements for the certification of quality management system upon China National Accreditation Service for Conformity Assessment (CNAS). As an organizer in National Proficiency Testing Programme for Aquatic Animal Disease Laboratories in Aquatic Animal Epidemic Prevention System of China 2016, the laboratory was entrusted by Chinese Agriculture Ministry with organizing WSD and IHHN tests. In terms of provider, we have a better understanding of the quality assurance of ISO/IEC Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? National/ International Title of event Co-organiser Date (mm/yy) Location. Participants International The International Workshop on Aquaculture Health no July, 2016 Qingdao, China 80 participants International FAO/NFTEC/YSFRI Workshop on Emergency Preparedness and Response and Contingency Planning FAO, National Fisheries Technology Extension Center of China vember, 2016 Beijing, China 40 participants 19. Did your laboratory participate in scientific meetings on behalf of the OIE? 10 OIE Reference Laboratory Reports Activities, 2016

11 Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Meeting of the OIE ad hoc Group on the OIE Manual of Diagnostic Tests for Aquatic Animals April, 2016 Paris, France Invited expert Meeting discussion on the OIE Aquatic Manual ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 OIE Reference Laboratory Reports Activities,

12 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries To organise National Testing Programme for Aquatic Animal Disease Laboratories in Aquatic Animal Epidemic Prevention System of China 78 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Participating of the meeting and provide experties for the OIE ad hoc Group on the OIE Manual of Diagnostic Tests for Aquatic Animals Paris, France The OIE ad hoc Group on the OIE Manual of Diagnostic Tests for Aquatic Animals 25. Additional comments regarding your report: There were other 10 domestic training courses and workshops attended and provided with lectures which are not listed in this report. There were 11 domestic farm visitings and expert advice in technical consultancies which are not listed in this report. It's suggested to add some international or regional organisation in the list of countries in the table under "9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?" 12 OIE Reference Laboratory Reports Activities, 2016