OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2017 This report has been submitted : :58:21 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Theileriosis Address of laboratory: Istituto Zooprofilattico Sperimentale della Sicilia (IZSSi), Italian Reference Centre for Anaplasma, Babesia, Rickettsia, (C.R.A.Ba.R.T.), via Gino Marinuzzi 3, 909, Palermo ITALY Tel.: Fax: address: santo.caracappa@izssicilia.it Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Santo Caracappa DVM, Health Direcor of IZSSi, Director of National Reference Center for Anaplasma, Babesia, Rickettsia and (CRABaRT) Name (including Title and Position) of OIE Reference Expert: Dr. Santo Caracappa DVM, IZSSi Health Direcor, Director of National Reference Center for Anaplasma, Babesia, Rickettsia and CRABaRT) Dr. Alessandra Torina, PhD, M.Sc, Director of IZSSi Laboratory of Entomology and Environmental Vector Control Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally equi IFI annulata IFI Direct diagnostic tests Nationally Internationally PCR annulata (30kDa surface antigen gene) YES PCR real time annulata NO PCR ovis NO PCR Theileiria equi NO 18 0 spp. blood smears YES Babesia spp. and spp. Reverse Line Blot YES ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2017

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4 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients annulata Positive Serum IFI Provided 1 ml 0.4 ml 3 annulata Positive Control PCR Produced 0.2 ml 0.2 ml 2 equi Positive serum IFI Provided 1 ml 0.5 ml 3 equi Positive Control PCR Produced 0.2 ml 0 1 ovis Positive Control PCR Produced 0.6 ml 0.3 ml 2 annulata positive control Real Time PCR Produced 0.2 ml OIE Reference Laboratory Reports Activities, 2017

5 annulata negative serum IFI Provided 1 ml 0.4 ml 3 equi negative serum IFI Provided 1 ml 0.5 ml 3 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) annulata Real Time PCR Modified from: Ros-García, A., Nicolás, A., García-Pérez, A. L., Juste, R. A., & Hurtado, A., Development and evaluation of a real-time PCR assay for the quantitative detection of annulata in cattle. Parasites & Vectors 20, 5(1), 171. Fluorogenic probe-based PCR assays Criado-Fornelio A, Buling A, Asenzo G, Benitez D, Florin-Christensen M, Gonzalez-Oliva A, Henriques G, Silva M, Alongi A, Agnone A, Torina A, Madruga CR. Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids. Vet Parasitol Jun 10;162(3-4): OIE Reference Laboratory Reports Activities,

6 ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses GREECE January MOROCCO January 48 0 FRANCE June LEBANON July 1 0 UNITED ARAB EMIRATES September 7 0 TUNISIA October Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided UNITED ARAB EMIRATES Recommendation for serological and molecular tests for the detection of spp. Training INDIA Consultancy on symptoms of Theileriosis and vaccine use Consultation and reference material LEBANON Recommendations on molecular diagnosis Reference material SPAIN New diagnostic tools on Theileriosis and their limits Consultation VIETNAM Molecular and serological diagnosis of theileria orientalis Consultation and reference materials RUSSIA Request of theileria spp reference material for molecular investigation Reference material KAZAKHSTAN laboratory methods Consultation 6 OIE Reference Laboratory Reports Activities, 2017

7 ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

8 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country in ticks from lebanon Characterizing tick borne in ticks in lebanon Lebanese agricultural research institut LEBANON Instituto de Investigación en Recursos Cinegéticos: IREC SPAIN Institute of Infection and Global Health, University of LiverpoolLiverpool, UK. UNITED KINGDOM Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State UniversityStillwater, OK, USA. UNITED STATES OF AMERICA UMR BIPAR INRA-ANSES- ENVAMaisons-Alfort, France. FRANCE Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universida de va de Lisboa,Lisboa, Portugal PORTUGAL Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin,Berlin, Germany. GERMANY Department of Microbiology, Medical School, Aristotle University of Thessaloniki,Thessaloniki, Greece. GREECE Biology Centre, Czech Academy of Sciences, Institute of ParasitologyCeske Budejovice, Czechia. CZECH REPUBLIC 8 OIE Reference Laboratory Reports Activities, 2017

9 Mise en place d'une plate forme et d'un réseau tunisoitalien pour la surveillance des maladies émergentes transmises par les tiques et les Culicidae (moustiques) 18 Development of a monitoring system, based on a crossborder network for the epidemiological surveillance which will allow, through a database (containing census of the cases, the presence of carriers, prevalence, environmental data... ), to follow the evolution of zoonosis and vector borne diseases and therefore constitute an early warning system Institute Pasteur de Tunis TUNISIA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control?. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 de la Fuente J, Antunes S, Bonnet S, Cabezas-Cruz A, Domingos AG, Estrada-Peña A, Johnson N, Kocan KM, Mansfield KL, Nijhof AM, Papa A, Rudenko N, Villar M, Alberdi P, Torina A, Ayllón N, Vancova M, Golovchenko M, Grubhoffer L, Caracappa S, Fooks AR, Gortazar C, Rego ROM. Tick-Pathogen Interactions and Vector Competence: Front Cell Infect Microbiol Apr 7;7:114. doi: /fcimb ecollection Review. Dabaja MF, Tempesta M, Bayan A, Vesco G, Vesco G, Greco G, Torina A, Blanda V, La Russa F, Scimeca S, Ezzedine M, Mortada H, Raoult D, Fournier PE, Mortada M. Diversity and distribution of ticks from domestic ruminants in Lebanon. Vet Ital Jun 30;53(2): b) International conferences: 3 An overview on Theileriosis: new diagnostic tools and their limits. Alessandra Torina. "APICOWPLEXA th International Meeting on Apicomplexa in Farm Animals," October 2017, Madrid, Spain. Systematic analysis of bovine tickborne diseases in Sicilian Territories. Santo Caracappa, Valeria Blanda, Maria Flaminia Persichetti, Michelangelo Auteri, Rosalia D Agostino, Claudia De Maria, Vittoria Currò, Domenico Vicari, Alessandra Torina. One Health 9th Tick and Tick-borne Pathogen Conference and 1st Asia Rickettsia Conference (TTP9-APRC1), 27 agosto-1 settembre 2017, Cairns (Australia) OIE Reference Laboratory Reports Activities,

10 Alessandra Torina. Partecipation to the OIE meeting "Ad Hoc Group for ", 8-10 February, Paris (France). c) National conferences: 1 e Babesia neglected among neglected diseases. Alessandra Torina. Conference on Rickettsiosis and Neglected Diseases of animals and humans. Palermo, 5-6 July 2017 d) Other: (Provide website address or link to appropriate information) 0 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 6 b) Seminars: 3 c) Hands-on training courses: 2 d) Internships (>1 month): 8 10 OIE Reference Laboratory Reports Activities, 2017

11 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country c United Arab Emirates 2 d Germany 3 a Lebanon 1 b Spain 2 a Greece 1 a Germany 1 a Spain 1 d Japan 1 d Bulgaria 2 d Greece 1 a Switzerland 1 a USA 1 b USA 1 d Spain 1 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Certificato di Accreditamento n (1).pdf 16. Is your laboratory accredited by an international accreditation body? OIE Reference Laboratory Reports Activities,

12 Test for which your laboratory is accredited PCR annulata PCR equi IFI equi spp. smears Accreditation body ACCREDIA ACCREDIA ACCREDIA ACCREDIA 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Ad Hoc Group for 02/17 Paris, France Speaker An update of Diagnostic methods for Theileriosis First CAMENET consultative meeting 11/17 Abu Dhabi - United Arab Emirates Speaker Collection, preservation and transport of specimen ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? OIE Reference Laboratory Reports Activities, 2017

13 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries To confirm test proficiencies for the detection of T. equi antibodies by IFAT 2 ToR : To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? OIE Reference Laboratory Reports Activities,

14 Kind of consultancy Location Subject (facultative) Technical consultancy on diagnostic methods Paris, France Ad Hoc Group for Twinning program on Camel diseases Palermo, Italy Training to researchers from United Arab Emirates First CAMENET consultative meeting Abu Dhabi - United Arab Emirates Lesson on vector collection, preservation and transport 25. Additional comments regarding your report: 14 OIE Reference Laboratory Reports Activities, 2017