Products. Established in 2006 in the heart of. Angio Proteomie wishes to be your

Size: px
Start display at page:

Download "Products. Established in 2006 in the heart of. Angio Proteomie wishes to be your"

Transcription

1 Established in 2006 in the heart of Longwood Medical Center of Harvard Medical School (Boston, MA 02215, US), Angio Proteomie, has since being dedicated in offering TOP QUALITY Research Reagents for Life Sciences communities. Angio Proteomie is currently offering > 5000 products ranging from monoclonal and antigen affinity purified polyclonal antibodies, primary human and animal cells, recombinant proteins, pre made adenoviral particles, and sirna. In addition, Angio Proteomie also offers custom based services in antibody development & production, primary cell and cell line development, protein expression & production, recombinant virus production. Products Angio Proteomie wishes to be your partner in your exciting scientific explorations. For details about our products and services, please contact us or visit our website.

2 Mouse Monoclonal anti Human Antibodies ( > 1000 products) hap 0001 hap 0002 hap 0003 hap 0004 hap 0005 Mouse anti human VEGFR 1 [MM0001 7G96] Mouse anti human VEGFR 2 [MM0002 2F66] Mouse anti human VEGFR 3 [MM0003 7G63] Mouse anti humanpdgfr alpha [MM0004 8A89] Mouse anti human PDGFR beta [MM0005 5C37] hap 1119 Mouse monoclonal anti human YY1 [MM1119] Feature of our monoclonal antibodies: Higher Specificity: Monoclonal antibodies and strictly validated Less Lot to Lot Variation: In vitro culture produced Higher Stability: Lyophlized format Low Cost: Competitively priced

3 Rat Monoclonal anti Mouse Antibodies ( > 570 products) map 0001 map 0002 map 0003 map 0004 map 0005 Rat anti mouse VEGFR 1 [RM0001 5F13] Rat anti mouse VEGFR 2 [RM0002 7A23] Rat anti mouse VEGFR 3 [RM0003 5F63] Rat anti mouse PDGFR alpha [RM0004 3G28] Rat anti mouse PDGFR beta [RM0005 4C54] map 0577 Rat monoclonal anti mouse Interleukin 9 [RM T32] Feature of our monoclonal antibodies: Higher Specificity: Monoclonal antibodies and strictly validated Less Lot to Lot Variation: In vitro culture produced Higher Stability: Lyophlized format Low Cost: Competitively priced

4 Functional Abs as Agonists or Antagonists (16 unique abs) mt1001m h mp1001r m Endoglin (Human, monoclonal, antagonistic) PECAM 1 (Mouse, monoclonal, antagonistic) pv1006r r VEGF C (Rat, Polyclonal, Blocking) mv1004.1mh/m mv1003m m VEGFR 1 (Human/Mouse, Monoclonal, Antagonistic) VEGFR 3 (Mouse, monoclonal, Antagonistic) mt1002r m Tie2 (Mouse, monoclonal, agonistic) Feature of our Functional Antibodies: Higher Specificity: For specific Ligands/Receptors Functional Assay Validated Higher Stability: Lyophlized format Only Available from Angio Proteomie

5 Sheep Polyclonal anti Human and Mouse Antibodies ( > 1000 Products) hap 5001 hap 5002 Polyclonal Sheep anti Human HSD17B1 Polyclonal Sheep anti Human 53BP1 map 5001 Polyclonal Sheep anti Mouse beta map 5002 Polyclonal Sheep anti Mouse epsilon hap 5565 map 5529 Polyclonal Sheep anti Human ZSCAN21 Polyclonal Sheep anti Mouse ZNF281 Feature of our Functional Antibodies: Higher Specificity: Antigen Affinity Purified Antibodies Higher Stability: Lyophlized format Small Package Size: Low cost

6 Human and Animal Primary Cells Human Retinal MV Pericytes Human Liver Stellate Cells cap 0001 cap 0002 cap 0003 cap 0004 Human Umbilical Vein Endothelial cells Human Brain Microvascular Endothelial Cells Human Dermal Lymphatic Human Glomerular cap 0034 Human MDA MB 231 Breast Cancer Cells cap 0035 Human Liver Stellate Cells Human Brain MV ECs Human Liver Sinusoidal MV ECs Feature of our Human and Animal Primary Cells: Early Passage Cells Fully Characterized Competitively Priced

7 Human and Animal Primary Cells (GFP or RFP tagged cells) RFP Tagged Human Retinal MV Pericytes cap 0001GFP cap 0001RFP cap 0002GFP cap 0004GFP GFP Tagged Human Umbilical Vein Endothelial cells RFP Tagged Human Umbilical Vein Endothelial cells GFP Tagged Human Brain Human Glomerular cap 0034GFP GFP Tagged Human MDA MB 231 Breast Cancer Cells GFP Tagged Human Placental Pericytes cap 0026GFP GFP Tagged Human Aorta Smooth Muscle Cells GFP tagged Human Aorta SMCs RFP Tagged Human Aorta SMCs Feature of our GFP or RFP Tagged Human and Animal Primary Cells: Early Passage Cells Stable Expression of GFP or RFP Competitively Priced

8 Human and Animal Primary Cells (GFP or RFP tagged cells) RFP Tagged Human Retinal MV Pericytes cap 0001GFP cap 0001RFP cap 0002GFP cap 0004GFP GFP Tagged Human Umbilical Vein Endothelial cells RFP Tagged Human Umbilical Vein Endothelial cells GFP Tagged Human Brain Human Glomerular cap 0034GFP GFP Tagged Human MDA MB 231 Breast Cancer Cells GFP Tagged Human Placental Pericytes cap 0026GFP GFP Tagged Human Aorta Smooth Muscle Cells GFP tagged Human Aorta SMCs RFP Tagged Human Aorta SMCs Feature of our GFP or RFP Tagged Human and Animal Primary Cells: Early Passage Cells Stable Expression of GFP or RFP Competitively Priced

cardiovascular cell Solutions

cardiovascular cell Solutions The Essentials of Life Science Research Globally Delivered cardiovascular cell Solutions We love what we do. And now we do it even better! As part of your portfolio for success, ATCC now provides a range

More information

Supplementary Fig. 1. Multiple five micron sections of liver tissues of rats treated

Supplementary Fig. 1. Multiple five micron sections of liver tissues of rats treated Supplementary Figure Legends Supplementary Fig. 1. Multiple five micron sections of liver tissues of rats treated with either vehicle (left; n=3) or CCl 4 (right; n=3) were co-immunostained for NRP-1 (green)

More information

Develop Better Assays for Every Human Protein

Develop Better Assays for Every Human Protein Develop Better Assays for Every Human Protein OriGene overview Application of transfected over-expression lysates Application of purified recombinant human proteins About OriGene Started in1996 with the

More information

Custom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service

Custom Antibody Services. Antibodies Designed Just for You. HuCAL Recombinant Monoclonal Antibody Generation Service Custom Antibody Services Antibodies Designed Just for You HuCAL Recombinant Monoclonal Antibody Generation Service Antibodies Designed Just for You What if there was a technology that would allow you to

More information

Thermo Scientific DharmaFECT Transfection Reagents sirna Transfection Protocol

Thermo Scientific DharmaFECT Transfection Reagents sirna Transfection Protocol Protocol Thermo Scientific Transfection Reagents sirna Transfection Protocol The following is a general protocol for use of Thermo Scientific transfection reagents to deliver sirna into cultured mammalian

More information

Antibody Validation by Western Blot. Supplementary information for Bergsma et al, Antibody Array Interaction Mapping

Antibody Validation by Western Blot. Supplementary information for Bergsma et al, Antibody Array Interaction Mapping Antibody Validation by Western Blot Supplementary information for Bergsma et al, Antibody Array Interaction Mapping Validation summary The antibodies were purified and tested by Western blot using various

More information

Your Antibody Source. prosci-inc.com. Extensive Antibody Services Broad Antibody Catalog

Your Antibody Source. prosci-inc.com. Extensive Antibody Services Broad Antibody Catalog Your Source prosci-inc.com Extensive Services Broad Catalog Company Overview Established in 1998, ProSci Incorporated is a leading provider of high performance antibodies and custom antibody services.

More information

Chapter 8 Cell Diversity

Chapter 8 Cell Diversity Chapter 8 Cell Diversity Mr. C. Biology 1 Future? Chapter 8 Cell Diversity Cells, Tissues, Organs and Systems Cells have different shapes because they have different jobs to do. A nerve cell is very different

More information

Antibody Project Portfolio

Antibody Project Portfolio Antibody Project Portfolio University of Abcam Generation date: July 1, 2014 Prepared for: Prof. J. Milner, University of Abcam Prepared by: Abcam US Scientific Support Team 1 Kendall Square Cambridge,

More information

Adhesion molecules and cytokines

Adhesion molecules and cytokines Adhesion molecules and cytokines Hycult Biotech is a leading world-class manufacturer of research reagents in the field of innate immunity. We are a specialized partner in the development and manufacturing

More information

Input. Pulldown GST. GST-Ahi1

Input. Pulldown GST. GST-Ahi1 A kd 250 150 100 75 50 37 25 -GST-Ahi1 +GST-Ahi1 kd 148 98 64 50 37 22 GST GST-Ahi1 C kd 250 148 98 64 50 Control Input Hap1A Hap1 Pulldown GST GST-Ahi1 Hap1A Hap1 Hap1A Hap1 Figure S1. (A) Ahi1 western

More information

Product Data Sheet - ANTIBODY

Product Data Sheet - ANTIBODY 888.267.4436 techsupport@origene.com www.origene.com Name:Mouse Monoclonal beta Actin Antibody Product Data Sheet - ANTIBODY Catalog: TA346894 Components: Mouse Monoclonal beta Actin Antibody (TA346894)

More information

The Molecular Virology Support Core: Adenoviral Vectors and Beyond

The Molecular Virology Support Core: Adenoviral Vectors and Beyond The : Adenoviral Vectors and Beyond Christoph A. Kahl, Ph.D. Viral Vector Workshop, May 5th, 2011 Outline 1) Overview of the MVSC 2) Adenoviral Vectors 3) Expertise and Services Overview What does the

More information

FORENSIC SEROLOGY. Chapter PRENTICE HALL 2008 Pearson Education, Inc. Upper Saddle River, NJ 07458

FORENSIC SEROLOGY. Chapter PRENTICE HALL 2008 Pearson Education, Inc. Upper Saddle River, NJ 07458 Chapter 8 FORENSIC SEROLOGY 8-1 Nature of Blood The word blood refers to a highly complex mixture of cells, enzymes, proteins, and inorganic substances. Plasma, which is the fluid portion of blood, is

More information

Solutions for Your Research

Solutions for Your Research Solutions for Your Research Custom Antibody Services Polyclonal Monoclonal The service we offer is very complete starting from rabbits, mice and rats. The different formats provided by Primm depend on

More information

Title: Production and characterisation of monoclonal antibodies against RAI3 and its expression in human breast cancer

Title: Production and characterisation of monoclonal antibodies against RAI3 and its expression in human breast cancer Author's response to reviews Title: Production and characterisation of monoclonal antibodies against RAI3 and its expression in human breast cancer Authors: Hannah Jörißen (hannah.joerissen@molbiotech.rwth-aachen.de)

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Name:CD34 mouse monoclonal antibody, clone OTI12F2 (formerly 12F2) Product Data Sheet - TRUEMAB Catalog: TA808864 Components: CD34 mouse monoclonal

More information

Proteomics. Areas of Application for Proteomics. Most Commonly Used Proteomics Techniques: Limitations: Examples

Proteomics. Areas of Application for Proteomics. Most Commonly Used Proteomics Techniques: Limitations: Examples Proteomics Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Antibody arrays Protein activity arrays 2-D gels ICAT technology SELDI Limitations: protein sources surfaces and

More information

Supporting information. Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo

Supporting information. Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo Supporting information Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo Greg Thurber 1, Katy Yang 1, Thomas Reiner 1, Rainer Kohler 1, Peter Sorger 2, Tim Mitchison

More information

Guide to Clonetics and Poietics Cell Types

Guide to Clonetics and Poietics Cell Types Guide to Clonetics and Poietics Cell Types Clonetics and Poietics Cell Types - Sorted By Tissue Source Cryopreserved Human Cells Adipose Subcutaneous Preadipocytes PT-5001 PGM -2 PT-8002 Visceral Preadipocytes

More information

How to run Alpha assay: How to setup an Alpha assay Make your own assay!

How to run Alpha assay: How to setup an Alpha assay Make your own assay! How to run Alpha assay: How to setup an Alpha assay Make your own assay! 1 2009 PerkinElmer AlphaLISA kits - recommendations before starting the assay Samples: Phenol red and hemoglobin: choose AlphaLISA

More information

Table S1. List of antibodies used including isotype controls, biotinylated. secondaries, and fluorophore conjugated tertiary antibodies.

Table S1. List of antibodies used including isotype controls, biotinylated. secondaries, and fluorophore conjugated tertiary antibodies. Table S1. List of antibodies used including isotype controls, biotinylated secondaries, and fluorophore conjugated tertiary antibodies. Antibody Description Distributor Catalogue number Working Concentration

More information

Adhesion molecules, cytokines & chemokines

Adhesion molecules, cytokines & chemokines Adhesion molecules, cytokines & chemokines Adhesion molecules and cytokines Hycult Biotech is a leading world-class manufacturer of research reagents in the field of innate immunity. We are a specialized

More information

MATF Antigen Submission Details and Standard Project Deliverables

MATF Antigen Submission Details and Standard Project Deliverables MATF Antigen Submission Details and Standard Project Deliverables What we require when you submit your antigen: Proteins For a recombinant protein target, we require a minimum of 400µg soluble recombinant

More information

Cell were phenotyped using FITC-conjugated anti-human CD3 (Pharmingen, UK)

Cell were phenotyped using FITC-conjugated anti-human CD3 (Pharmingen, UK) SUPPLEMENTAL MATERIAL Supplemental Methods Flow cytometry Cell were phenotyped using FITC-conjugated anti-human CD3 (Pharmingen, UK) and anti-human CD68 (Dako, Denmark), anti-human smooth muscle cell α-actin

More information

VERIFY Tagged Antigen. Validation Data

VERIFY Tagged Antigen. Validation Data VERIFY Tagged Antigen Validation Data Antibody Validation Figure 1. Over-expression cell lysate for human STAT3 (NM_139276) was used to test 3 commercial antibodies. Antibody A shows strong antigen binding.

More information

ICANtibodies TM. Has discovered more than 200 different antibodies for less than 2 years.

ICANtibodies TM. Has discovered more than 200 different antibodies for less than 2 years. An innovative antibody development technology using breakthrough nanotaxis leading to the antigen expression by the chosen animal species. Has discovered more than 200 different antibodies for less than

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Name:MUC1 (EMA) mouse monoclonal antibody, clone OTI2E3 (formerly 2E3) Product Data Sheet - TRUEMAB Catalog: TA800794 Components: MUC1 (EMA) mouse monoclonal

More information

Matrices sourcebook. Your guide to Gibco extracellular matrices products

Matrices sourcebook. Your guide to Gibco extracellular matrices products Matrices sourcebook Your guide to Gibco extracellular matrices products Introduction Gibco products, including extracellular matrices, 3D scaffolds, and attachment proteins, are essential tools for providing

More information

Chapter 4. Future Directions

Chapter 4. Future Directions 131 Chapter 4 Future Directions 132 Investigation of ephrin-b2 function in arterial smooth muscle cells Ephrin-B2 is expressed in arterial smooth muscle cells (SMCs) as well as in arterial endothelial

More information

Product Datasheet. Integrin alpha 3/CD49c Antibody NBP Unit Size: 0.1 ml

Product Datasheet. Integrin alpha 3/CD49c Antibody NBP Unit Size: 0.1 ml Product Datasheet Integrin alpha 3/CD49c Antibody NBP2-48514 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Protocols, Publications, Related Products,

More information

Develop Better Assays for Every Human Protein. OriGene Technologies, Inc.

Develop Better Assays for Every Human Protein. OriGene Technologies, Inc. Develop Better Assays for Every Human Protein OriGene Technologies, Inc. About OriGene Started in1996 with the goal to create largest commercial collection of full-length human cdnas Develop technologies

More information

64 CuCl 2 in 50 µl 0.1N NaOAc buffer, and 20 µg of each DOTA-antibody conjugate in 40 µl

64 CuCl 2 in 50 µl 0.1N NaOAc buffer, and 20 µg of each DOTA-antibody conjugate in 40 µl Number of DOTA per antibody The average number of DOTA chelators per antibody was measured using a reported procedure with modifications (1,2). Briefly, nonradioactive CuCl 2 (80-fold excess of DOTA antibodies)

More information

Product Manual. RFP ELISA Kit. Catalog Number. FOR RESEARCH USE ONLY Not for use in diagnostic procedures

Product Manual. RFP ELISA Kit. Catalog Number. FOR RESEARCH USE ONLY Not for use in diagnostic procedures Product Manual RFP ELISA Kit Catalog Number AKR-122 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Red fluorescent protein (DsRed) is a spontaneously fluorescent protein

More information

PROTOCOL 1850 Millrace Drive, Suite 3A Eugene, Oregon

PROTOCOL 1850 Millrace Drive, Suite 3A Eugene, Oregon PROTOCOL ATP Synthase Protein Quantity Microplate Assay Kit 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 Rev.0 DESCRIPTION ATP Synthase Protein Quantity Microplate Assay Kit Sufficient materials

More information

Chapter 8 Healthcare Biotechnology

Chapter 8 Healthcare Biotechnology Chapter 8 Healthcare Biotechnology Outline: 8.1 Introduction 8.2 Biopharming 8.3 Models of Human Disease 8.4 Detecting and Diagnosing Human Disease 8.5 Monoclonal Antibodies 8.6 Gene Therapy 8.7 Tissue

More information

Mouse TNF Alpha PicoKine ELISA Kit

Mouse TNF Alpha PicoKine ELISA Kit BOSTER BIOLOGICAL TECHNOLOGY 3942 B Valley Ave, Pleasanton, CA 94566 Phone: 888-466-3604 Fax: 925-215-2184 Email: boster@bosterbio.com Web: www.bosterbio.com Mouse TNF Alpha PicoKine ELISA Kit Catalog

More information

Matrices Sourcebook Life Technologies extracellular matrices products

Matrices Sourcebook Life Technologies extracellular matrices products Matrices Sourcebook Life Technologies extracellular matrices products Introduction Our Gibco products, including extracellular matrices, 3D scaffolds, and attachment proteins, are essential tools for providing

More information

Histone H3 Methylation Antibody Panel Pack II - Repression Genes Base Catalog # C10003

Histone H3 Methylation Antibody Panel Pack II - Repression Genes Base Catalog # C10003 Histone H3 Methylation Antibody Panel Pack II - Repression Genes Base Catalog # PACK CONTENTS Component Size Shipping Temperature Upon Receipt Checklist 3R2DA Histone H3R2 Dimethyl Asymmetric (H3R2me2a)

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB Name:Mouse monoclonal anti-pcna antibody, clone 4B2, Loading, clone OTI4B2 (formerly 4B2) Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Catalog: TA800880 Components:

More information

Recombinant adenoviruses. A TRB3-expressing adenovirus was generated through

Recombinant adenoviruses. A TRB3-expressing adenovirus was generated through Materials and Methods Recombinant adenoviruses. A -expressing adenovirus was generated through homologous recombination between a linearized transfer vector pad-track and the adenoviral backbone vector

More information

Rat IL-1 Beta PicoKine ELISA Kit

Rat IL-1 Beta PicoKine ELISA Kit BOSTER BIOLOGICAL TECHNOLOGY 3942 B Valley Ave, Pleasanton, CA 94566 Phone: 888-466-3604 Fax: 925-215-2184 Email: boster@bosterbio.com Web: www.bosterbio.com Rat IL-1 Beta PicoKine ELISA Kit Catalog Number:

More information

Pharmacokinetics of PROTEIN DRUGS

Pharmacokinetics of PROTEIN DRUGS Pharmacokinetics of PROTEIN DRUGS PEPTIDE AND PROTEIN DRUGS In this lecture, the general differences in the kinetic behavior of protein drugs relative to that observed with small molecules is emphasized.

More information

Discovery on Target. Short Course Preview Deck

Discovery on Target. Short Course Preview Deck Discovery on Target Short Course Preview Deck Targeting of GPCRs with Monoclonal Antibodies Barbara Swanson, Ph.D. Sorrento Therapeutics, Inc. October 2014 GPCR Short Course Overview Introductions Brief

More information

Proteomics. Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Limitations: Examples

Proteomics. Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Limitations: Examples Proteomics Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Antibody arrays Protein activity arrays 2-D gels ICAT technology SELDI Limitations: protein sources surfaces and

More information

Areas of Application for Proteomics Most Commonly Used Proteomics Techniques:

Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Proteomics Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Antibody arrays Protein activity arrays 2-D gels ICAT technology SELDI Limitations: Examples protein sources surfaces

More information

Antibodies & Assays. Assay Development. Primary Antibodies. Conjugated Secondary Antibodes. Epitope Tag Antibodies. Streptavidin Reagents

Antibodies & Assays. Assay Development. Primary Antibodies. Conjugated Secondary Antibodes. Epitope Tag Antibodies. Streptavidin Reagents Antibodies & Assays Volume 33 Version 5 Assay Development Primary Antibodies Conjugated Secondary Antibodes Epitope Tag Antibodies Streptavidin Reagents For 50 years, ROCKLAND IMMUNOCHEMICALS Inc. has

More information

Supplementary Information. Conversion of vascular endothelial cells into multipotent stem-like cells

Supplementary Information. Conversion of vascular endothelial cells into multipotent stem-like cells Supplementary Information Conversion of vascular endothelial cells into multipotent stem-like cells Damian Medici 1, Eileen M. Shore 2,3,4, Vitali Y. Lounev 2,4, Frederick S. Kaplan 2,4,5, Raghu Kalluri

More information

Everything counts. But nothing counts like the

Everything counts. But nothing counts like the Everything counts But nothing counts like the Countess II FL Automated Cell Counter The new Countess II FL Automated Cell Counter is a benchtop cell assay platform equipped with state-of-the-art optics

More information

Standard deviation, calculated from results generated under repeatability conditions.

Standard deviation, calculated from results generated under repeatability conditions. OIV-MA-AS315-23 Type of Method: criteria CRITERIA FOR THE METHODS OF QUANTIFICATION OF POTENTIALLY ALLERGENIC RESIDUES OF FINING AGENT PROTEINS IN WINE (OIV-Oeno 427-2010 modified by OIV-COMEX 502-2012)

More information

ONE-HOUR Complete IP-Western Kit

ONE-HOUR Complete IP-Western Kit ONE-HOUR Complete IP-Western Kit Technical Manual No. 0218 Version 06192009 I Description.. 1 II Kit Contents.. 2 III Related Products 2 IV Key Features.. 2 V Storage.. 2 VI ONE-HOUR Western Protocol 3

More information

Make Your Immunology Research Easy. Kun YIN Associate Director of Marketing Division, GenScript

Make Your Immunology Research Easy. Kun YIN Associate Director of Marketing Division, GenScript Make Your Immunology Research Easy Kun YIN Associate Director of Marketing Division, GenScript GenScript Overview Branch Office Amsterdam, Netherlands Branch Office Tokyo, Japan Headquarter, New Jersey,

More information

Nature Medicine: doi: /nm.4169

Nature Medicine: doi: /nm.4169 Supplementary Fig.1. EC-specific deletion of Ccm3 by Cdh5-CreERT2. a. mt/mg reporter mice were bred with Cdh5CreERT2 deleter mice followed by tamoxifen feeding from P1 to P3. mg expression was specifically

More information

colorimetric sandwich ELISA kit datasheet

colorimetric sandwich ELISA kit datasheet colorimetric sandwich ELISA kit datasheet For the quantitative detection of human ENO2 concentrations in serum, plasma and cell culture supernatants. general information Catalogue Number Product Name Species

More information

Product Datasheet. ELK3 Antibody (OTI1H3) NBP Unit Size: 0.1 ml. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1

Product Datasheet. ELK3 Antibody (OTI1H3) NBP Unit Size: 0.1 ml. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1 Product Datasheet ELK3 Antibody (OTI1H3) NBP2-01264 Unit Size: 0.1 ml Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1 Protocols, Publications, Related Products, Reviews, Research Tools

More information

SUPPLEMENTAL MATERIAL. Supplemental Methods:

SUPPLEMENTAL MATERIAL. Supplemental Methods: SUPPLEMENTAL MATERIAL Supplemental Methods: Immunoprecipitation- As we described but with some modifications [22]. As part of another ongoing project, lysate from human umbilical vein endothelial cells

More information

TRANSFEX - SUPERIOR GENE EXPRESSION FOR HARD-TO-TRANSFECT CELL TYPES. Kevin Grady Product Line Business Manager ASCB Vendor Showcase Dec.

TRANSFEX - SUPERIOR GENE EXPRESSION FOR HARD-TO-TRANSFECT CELL TYPES. Kevin Grady Product Line Business Manager ASCB Vendor Showcase Dec. TRANSFEX - SUPERIOR GENE EXPRESSION FOR HARD-TO-TRANSFECT CELL TYPES Kevin Grady Product Line Business Manager ASCB Vendor Showcase Dec. 15, 2013 Outline Overview of transfection TransfeX Primary/hTERT

More information

Supplementary Information (Ha, et. al) Supplementary Figures Supplementary Fig. S1

Supplementary Information (Ha, et. al) Supplementary Figures Supplementary Fig. S1 Supplementary Information (Ha, et. al) Supplementary Figures Supplementary Fig. S1 a His-ORMDL3 ~ 17 His-ORMDL3 GST-ORMDL3 - + - + IPTG GST-ORMDL3 ~ b Integrated Density (ORMDL3/ -actin) 0.4 0.3 0.2 0.1

More information

SPHERO TM Coated Particles

SPHERO TM Coated Particles SPHERO TM Coated Particles Manufactured by either passive adsorption or covalent coupling depending upon the intended application Stable for several years under proper storage condition Available in a

More information

ELISPOT and FLUOROSPOT kits

ELISPOT and FLUOROSPOT kits ELISPOT and FLUOROSPOT kits Interleukins Interferons Granzymes and perforins TNF superfamily ligands and receptors Apoptosis markers And many more... ELISPOT and FLUOROSPOT: a cell-based assay to assess

More information

Methods to Determine the Binding of Bevacizumab to Fc receptors

Methods to Determine the Binding of Bevacizumab to Fc receptors DATASHEET Methods to Determine the Binding of Bevacizumab to Fc receptors BACKGROUND Bevacizumab (Avastin ) is a humanized recombinant monoclonal antibody that inhibits angiogenesis by binding to the vascular

More information

CellPlayer CytoLight Green (Lenti, CMV, no selection)

CellPlayer CytoLight Green (Lenti, CMV, no selection) CellPlayer CytoLight Green (Lenti, CMV, no selection) Essen BioScience Catalog Number: 4513 Background Third generation lentiviral-based vectors are commonly used to transfer genetic information to cells

More information

Ultrasound Contrast Agents for Molecular Imaging

Ultrasound Contrast Agents for Molecular Imaging Ultrasound Contrast Agents for Molecular Imaging Design and Development Joshua Rychak, PhD Vice President for Research Targeson, Inc Adj. Assistant Professor Dept of Bioengineering University of California,

More information

Supplementary Methods

Supplementary Methods Supplementary Methods Antibodies Rabbit polyclonal VEGFR-1, VEGFR-2, Angiopoietin-1, Angiopoietin- 2 and GAPDH specific antibodies were from Santa Cruz Biotechnology. Rabbit polyclonal Akt1, Akt2, ps473

More information

Structure of IgG and IgM

Structure of IgG and IgM Structure of IgG and IgM Fig. 5-1 A,B Crystal Structure of Secreted IgG Fig. 5-1 C Structure of an Ig Domain Fig. 5-2 Proteolytic Fragments of IgG (1) Fig. 5-3A Proteolytic Fragments of IgG (2) Fig. 5-3B

More information

Product Data Sheet - UltraMAB

Product Data Sheet - UltraMAB Name:FOLH1 mouse monoclonal antibody,clone UMAB25 Product Data Sheet - UltraMAB Catalog: UM570025 Properties Components: Amount: Immunogen: Host: Clone Name: Isotype: Species Reactivity: Guaranteeded Application:

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Name:IL6 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9) Product Data Sheet - TRUEMAB Catalog: TA500067 Components: IL6 mouse monoclonal antibody,

More information

Examination in Immunotechnology, 30 May 2011, 8-13

Examination in Immunotechnology, 30 May 2011, 8-13 Examination in Immunotechnology, 30 May 2011, 8-13 1 Each question can give 5p, with a total of 10 questions (i.e. 50 points in total). 2 Write name and personal number on ALL pages (including the cover).

More information

SPHERO TM Coated Particles

SPHERO TM Coated Particles SPHERO TM Coated Particles Manufactured by either passive adsorption or covalent coupling depending upon the intended application Stable for several years under proper storage condition Available in a

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Name:MUC1 (EMA) mouse monoclonal antibody, clone OTI2F6 (formerly 2F6) Product Data Sheet - TRUEMAB Catalog: TA800790 Components: MUC1 (EMA) mouse monoclonal

More information

Product Datasheet. Occludin Antibody NBP Unit Size: 0.1 ml

Product Datasheet. Occludin Antibody NBP Unit Size: 0.1 ml Product Datasheet Occludin Antibody NBP1-87402 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 2 Publications: 2 Protocols, Publications,

More information

PicoKine ELISA. Catalog number: EK0527

PicoKine ELISA. Catalog number: EK0527 PicoKine ELISA Catalog number: EK0527 For the quantitation of Mouse Tnf concentrations in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This package insert must be read in its

More information

Purification Kits. Fast and Convenient PROSEP -A and PROSEP-G Spin Column Kits for Antibody Purification DATA SHEET

Purification Kits. Fast and Convenient PROSEP -A and PROSEP-G Spin Column Kits for Antibody Purification DATA SHEET Â Montage Antibody Purification Kits Fast and Convenient PROSEP -A and PROSEP-G Spin Column Kits for Antibody Purification DATA SHEET Available with immobilized Protein A or Protein G Easy-to-use Antibody

More information

Product Datasheet. PKC alpha Antibody (2F11) H M01. Unit Size: 0.1 mg. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Datasheet. PKC alpha Antibody (2F11) H M01. Unit Size: 0.1 mg. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Product Datasheet PKC alpha Antibody (2F11) H00005578-M01 Unit Size: 0.1 mg Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Protocols, Publications, Related Products, Reviews, Research Tools

More information

Product Data Sheet - TRUEMAB

Product Data Sheet - TRUEMAB 888.267.4436 techsupport@origene.com www.origene.com Name:PFKP mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) Product Data Sheet - TRUEMAB Catalog: TA503979 Components: PFKP mouse monoclonal antibody,

More information

Histone H3K27 Methylation Antibody Panel Pack Base Catalog # C Component Size Shipping Temperature

Histone H3K27 Methylation Antibody Panel Pack Base Catalog # C Component Size Shipping Temperature Histone H3K27 Methylation Antibody Panel Pack Base Catalog # PACK CONTENTS Component Size Shipping Temperature Upon Receipt Checklist 3K27M Histone H3K27me1 (H3K27 Monomethyl) Polyclonal Antibody 25 µg

More information

Optimization of 2D Gel Transblotting for Host Cell Protein Analysis

Optimization of 2D Gel Transblotting for Host Cell Protein Analysis Optimization of 2D Gel Transblotting for Host Cell Protein Analysis Jon Johansen, Matt Hoelter & Nancy Kendrick* Kendrick Labs Inc, Madison, WI www.kendricklabs.com Talk Outline Biologic drugs, recombinant

More information

Supplementary Table 1. Primers used to construct full-length or various truncated mutants of ISG12b2.

Supplementary Table 1. Primers used to construct full-length or various truncated mutants of ISG12b2. Supplementary Table 1. Primers used to construct full-length or various truncated mutants of ISG12b2. Construct name ISG12b2 (No tag) HA-ISG12b2 (N-HA) ISG12b2-HA (C-HA; FL-HA) 94-283-HA (FL-GFP) 93-GFP

More information

Novel SCRG1/BST1 axis regulates. self-renewal, migration, and osteogenic differentiation potential. in mesenchymal stem cells

Novel SCRG1/BST1 axis regulates. self-renewal, migration, and osteogenic differentiation potential. in mesenchymal stem cells Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells Emiko Aomatsu 1,2, Noriko Takahashi 1, Shunsuke Sawada 3,4, Naoto Okubo 1,7,

More information

Supporting Information. HDL-mimetic PLGA nanoparticle for the targeting of atherosclerosis. plaque macrophages

Supporting Information. HDL-mimetic PLGA nanoparticle for the targeting of atherosclerosis. plaque macrophages Supporting Information HDL-mimetic PLGA nanoparticle for the targeting of atherosclerosis plaque macrophages Brenda L. Sanchez-Gaytan a*, Francois Fay a*, Mark E. Lobatto a,b*, Jun Tang a,c, Mireille Ouimet

More information

Product Datasheet. IL-6 Antibody (OTI3G9) NBP Unit Size: 0.1 ml. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1

Product Datasheet. IL-6 Antibody (OTI3G9) NBP Unit Size: 0.1 ml. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1 Product Datasheet IL-6 Antibody (OTI3G9) NBP1-47810 Unit Size: 0.1 ml Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 1 Protocols, Publications, Related Products, Reviews, Research Tools

More information

Biochemical Markers. Human Recombinant Proteins & Antibodies. Biochemical messengers Tumour markers Cardiovascular Immunology Metabolic Cerebral FABPs

Biochemical Markers. Human Recombinant Proteins & Antibodies. Biochemical messengers Tumour markers Cardiovascular Immunology Metabolic Cerebral FABPs Biochemical Markers Human Recombinant Proteins & Antibodies Biochemical messengers Tumour markers Cardiovascular Immunology Metabolic Cerebral FABPs Randox Life Sciences is recognised as a primary manufacturer

More information

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies

Antigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known

More information

Post-translational modification

Post-translational modification Protein expression Western blotting, is a widely used and accepted technique to detect levels of protein expression in a cell or tissue extract. This technique measures protein levels in a biological sample

More information

Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology

Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology Chapter 17: Immunization & Immune Testing 1. Immunization 2. Diagnostic Immunology 1. Immunization Chapter Reading pp. 505-511 What is Immunization? A method of inducing artificial immunity by exposing

More information

1. Immunization. What is Immunization? 12/9/2016. Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology

1. Immunization. What is Immunization? 12/9/2016. Chapter 17: Immunization & Immune Testing. 1. Immunization 2. Diagnostic Immunology Chapter 17: Immunization & Immune Testing 1. Immunization 2. Diagnostic Immunology 1. Immunization Chapter Reading pp. 505-511 What is Immunization? A method of inducing artificial immunity by exposing

More information

NSE Grantees Meeting December 2015

NSE Grantees Meeting December 2015 NSE Grantees Meeting December 2015 The Spherical Nucleic Acid (SNA) Nanoparticle Changes the Paradigm for Oligo Therapeutics Linear DNA SNA Each company limited by chemistry, modality, and tissue of interest

More information

Fig. S1. Effect of p120-catenin overexpression on the interaction of SCUBE2 with E-cadherin. The expression plasmid encoding FLAG.

Fig. S1. Effect of p120-catenin overexpression on the interaction of SCUBE2 with E-cadherin. The expression plasmid encoding FLAG. Fig. S1. Effect of p120-catenin overexpression on the interaction of SCUBE2 with E-cadherin. The expression plasmid encoding FLAG.SCUBE2, E-cadherin.Myc, or HA.p120-catenin was transfected in a combination

More information

Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney

Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney 1 Supplementary text and data for: 2 3 4 5 Cycles of vascular plexus formation within the nephrogenic zone of the developing mouse kidney Authors: David A. D. Munro 1*, Peter Hohenstein 2, and Jamie A.

More information

Antibody Profiling on Invitrogen ProtoArray High-Density Protein Microarrays

Antibody Profiling on Invitrogen ProtoArray High-Density Protein Microarrays 1 Antibody Profiling on Invitrogen ProtoArray High-Density Protein Microarrays The application of antibodies in research and development, in vitro/in vivo diagnostics, or therapeutics requires that antibodies

More information

HOST DEFENSE SMALL GROUP PROBLEM SOLVING SESSION CLINICAL IMMUNOLOGIC ASSAYS-II

HOST DEFENSE SMALL GROUP PROBLEM SOLVING SESSION CLINICAL IMMUNOLOGIC ASSAYS-II HOST DEFENSE SMALL GROUP PROBLEM SOLVING SESSION CLINICAL IMMUNOLOGIC ASSAYS-II Monday, March 24, 2008 2:00 PM 4:00 PM Small Group Classrooms LEARNING GOAL Understanding in vitro assessment of immunologic

More information

T-cell response. Taken from NIAID: s.aspx

T-cell response. Taken from NIAID:   s.aspx T-cell receptor T-cell response 1. Macrophage or dendritic cell digest antigen bacteria, virus 2. Fragments of Ag bind to major histo-compatiblity (MHC) proteins in macrophage. 3. MHC I-Ag fragment expressed

More information

Mouse TNFα ELISA Kit

Mouse TNFα ELISA Kit Mouse TNFα ELISA Kit CATALOG NO: IRKTAH2622 LOT NO: SAMPLE INTENDED USE For quantitative detection of mouse TNFα in cell culture supernates, serum and plasma (heparin, EDTA). BACKGROUND Tumor necrosis

More information

CD1a-autoreactive T cells are a normal component of the human αβ T cell repertoire

CD1a-autoreactive T cells are a normal component of the human αβ T cell repertoire CDa-autoreactive T cells are a normal component of the human αβ T cell repertoire Annemieke de Jong, Victor Peña-Cruz, Tan-Yun Cheng, Rachael A. Clark, Ildiko Van Rhijn,, D. Branch Moody Division of Rheumatology,

More information

Small molecule lysyl oxidase-like 2 (LOXL2) inhibitors: the identification of an inhibitor selective for LOXL2 over LOX Supporting Data LOX LOXL2

Small molecule lysyl oxidase-like 2 (LOXL2) inhibitors: the identification of an inhibitor selective for LOXL2 over LOX Supporting Data LOX LOXL2 Small molecule lysyl oxidase-like 2 (LOXL2) inhibitors: the identification of an inhibitor selective for LOXL2 over LOX John H. Hutchinson, *, Martin W. Rowbottom, David Lonergan, Janice Darlington, Pat

More information

Really Good Antibodies. Secondary Antibodies. Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies. bethyl.com

Really Good Antibodies. Secondary Antibodies. Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies. bethyl.com Really Good Antibodies Secondary Antibodies Over 670 Anti-Mouse, Rabbit, Goat and Rat Secondary Antibodies bethyl.com Bethyl Laboratories, Inc. has been dedicated to supporting scientific discovery through

More information

Antibodies and reagents Construction and characterization of YFP-TF chimera

Antibodies and reagents Construction and characterization of YFP-TF chimera Antibodies and reagents Antibodies: mouse monoclonal antibodies (mab) against PDI (clone 34, BD Transduction and RL90, Affinity Bioreagents); rabbit polyclonals against bovine PDI (SPA-890, Stressgen and

More information

ProteoGenix. Life Sciences Services and Products. From gene to biotherapeutics Target Validation to Lead optimisation

ProteoGenix. Life Sciences Services and Products. From gene to biotherapeutics Target Validation to Lead optimisation ProteoGenix Life Sciences Services and Products From gene to biotherapeutics Target Validation to Lead optimisation ProteoGenix Philippe FUNFROCK, founder and CEO French company located in Strasbourg,

More information

Life Sciences WNT PATHWAY. Integrated solutions for screening Wnt regulators. Image: TR Arnett, UCL

Life Sciences WNT PATHWAY. Integrated solutions for screening Wnt regulators. Image: TR Arnett, UCL Life Sciences WNT PATHWAY Integrated solutions for screening regulators Image: TR Arnett, UCL 3Find Your Total Solution SOLUTIONS FOR WNT PATHWAY SCREENING High-throughput Assays for Canonical Pathway

More information

Application of Biacore Technology

Application of Biacore Technology Principles and typical results Application of Biacore Technology Common types of Biacore analyses Specificity analysis Is my molecule of interest specific for its target? Multiple binding analysis In which

More information