Product Data Sheet - TRUEMAB

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1 Name:CD34 mouse monoclonal antibody, clone OTI12F2 (formerly 12F2) Product Data Sheet - TRUEMAB Catalog: TA Components: CD34 mouse monoclonal antibody, clone OTI12F2 (formerly 12F2) (TA808864) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids of human CD34(NP_ ) produced in E.coli. Host: Mouse Isotype: IgG2a Species Reactivity: Human Guaranteed Applications: IHC Suggested Dilutions: IHC 1:150, Concentration: 1.00mg/ml Buffer: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from cell culture supernatant by affinity chromatography Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Homo sapiens CD34 molecule (CD34), transcript variant 1 Alternative Name: Database Link: CD34 antigen; OTTHUMP ; OTTHUMP ; hematopoietic progenitor cell antigen CD34; CD34 molecule NP_ Entrez Gene 947 Human Entrez Gene 0 Mouse Entrez Gene 0 Rat Entrez Gene 0 Monkey Entrez Gene 0 Dog Function: The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and

2 phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].

3 Validation Data Human breast tissue within the normal limits using anti- Human colon tissue within the normal limits using anti- CD34 mouse monoclonal antibody. (Heat-induced Adenocarcinoma of Human colon tissue using anti-cd34 mouse monoclonal antibody. (Heat-induced epitope 120 C for 3 min, TA808864)(1:150)

4 Human liver tissue within the normal limits using anti- Carcinoma of Human liver tissue using anti-cd34 mouse monoclonal antibody.(heat-induced epitope 120 C for 3 min, TA808864)(1:150) Human lung tissue within the normal limits using anti-

5 Adenocarcinoma of Human ovary tissue using anti- (ph8.5) at 120 C for 3 min, TA808864)(1:150) Human pancreas tissue within the normal limits using anti-cd34 mouse monoclonal antibody. (Heat-induced Human thyroid tissue within the normal limits using anti-

6 Carcinoma of Human thyroid tissue using anti-cd34 mouse monoclonal antibody.(heat-induced epitope 120 C for 3 min, TA808864)(1:150) Adenocarcinoma of Human endometrium tissue using anti- (ph8.5) at 120 C for 3 min, TA808864)(1:150) Human lymph node tissue within the normal limits using anti-cd34 mouse monoclonal antibody. (Heat-induced

7 Human lymphoma tissue using anti-cd34 mouse monoclonal antibody.(heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (ph8.5) at 120 C for 3 min, TA808864)(1:150) Human tonsil within the normal limits using anti-cd34 mouse monoclonal antibody.(heat-induced epitope 120Â C for 3 min, TA808864)(1:150) * More validation images may be available on our website:

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