Soil and Water Department, Estación Experimental de Aula Dei (EEAD), Spanish National Research Council (CSIC)
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1 Irrigation and soil tillage management: effects on soil N 2 O emissions and microbial communities in a maize (Zea mays L.) monoculture under Mediterranean conditions Samuel Franco-Luesma 1 *, Elena Paracuellos 1, Daniel Plaza-Bonilla 1, José Luis Arrúe 1, Carlos Cantero-Martínez 2, Germán Tortosa 3, Eulogio J. Bedmar 3, José Cavero 1, Jorge Álvaro-Fuentes 1 1 Soil and Water Department, Estación Experimental de Aula Dei (EEAD), Spanish National Research Council (CSIC) 2 Crop and Forest Sciences Department, EEAD-CSIC Associated Unit, Agrotecnio, University of Lleida 3 Soil Microbiology and Symbiotic Systems Department, Estación Experimental del Zaidín (EEZ), (CSIC) * sfranco@eead.csic.es Red Sirena. 07 noviembre 2018, Madrid, España
2 Outline Introduction and Objective Material and Methods Results Conclusions Red Sirena 2 Madrid 07 November 2018
3 Outline Introduction and Objective Material and Methods Results Conclusions Red Sirena Madrid 07 November
4 Introduction Under Mediterranean conditions, high maize productivities largely depend on irrigation supply, large amounts of N fertilizer and intensive tillage practices Soil tillage and irrigation system may have a significant impact on soil physical properties, affecting soil water content and on the O 2 availability Impact N 2 O + Microbial communities Red Sirena 4 Madrid 07 November 2018
5 Objetive The objective of this work was to assess the impact of tillage and irrigation management on soil nitrous oxide (N 2 O) emissions and on the microbial communities in a 3-year maize (Zea mays L.) monoculture in a Mediterranean agroecosystem Red Sirena 5 Madrid 07 November 2018
6 Outline Introduction and Objective Material and Methods Results Conclusions Red Sirena Madrid 07 November
7 Localization of the experimental site Zaragoza, NE Spain Mean annual precipitation Mean annual air temperature Potential evapotranspiration 344 mm 14.8 ºC 1100 mm EEAD-CSIC Red Sirena 7 Madrid 07 November 2018
8 Experimental design 2 Irrigation systems Sprinkler (S) Flood (F) 3 Tillage systems (a) (b) Conventional tillage (CT) No-tillage without crop stover (NT) No-tillage with crop stover (NTr) Split-block with 3 replicates per treatment (plot size: 6 x 18 m) a) b) Red Sirena 8 Madrid 07 November 2018
9 Measurement campaigns 2015, 2016 and periods 2015 growing season Fallow Fallow growing season 2017 growing season Red Sirena Madrid 07 November
10 Agricultural management Measurement campaigns: 2015, 2016 and 2017 Fallow Growing season Fallow Pre-sowing application: 800 kg ha -1 NPK ( ) Top dressing application: 740 kg ha -1 CAN-27% Tillage Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec Sowing Harvest Tillage Red Sirena Madrid 07 November
11 GHG gas sampling frequency Measurement campaigns: 2015, 2016 and 2017 Fallow Growing season Fallow Fallow: every 21 days Growing season: weekly Fallow: every 21 days Fertilization and flood irrigation Increased sampling frequency Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec Red Sirena Madrid 07 November
12 Soil sampling for gene abundance analysis Fallow Measurement campaign: 2016 Growing season Fallow Pre-sowing application: 800 kg ha -1 NPK ( ) Top dressing application: 740 kg ha -1 NAC-27% 2 nd sampling 3 rd sampling 1 st sampling Tillage Jan Feb Mar Apr May Jun Jul Aug Sept Oct Nov Dec Sowing Harvest Tillage Red Sirena Madrid 07 November
13 Soil DNA extraction and gene abundance analysis 16 S Bacteria / Archaea Red Sirena 13 Madrid 07 November 2018
14 Thank you for your attention RED SIRENA Red Sirena Madrid 07 November
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