GST Tag Polyclonal Antibody Catalog Number Product data sheet

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1 Website: thermofisher.com Customer Service (US): ext. 1 Technical Support (US): ext. 441 GST Tag Polyclonal Antibody Catalog Number Product data sheet Details Size 100 µg Host/Isotope Class Type Immunogen Conjugate Form Concentration Purification Rabbit / IgG Polyclonal Antibody Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pgex vector Unconjugated Liquid 0.25 mg/ml Storage buffer PBS, ph 7.4 Contains Storage Conditions Antigen affinity chromatography 0.1% sodium azide -20 C Species Reactivity Tested species reactivity Tag Published species reactivity Human, Mouse, Not Applicable Tested Applications Dilution * ELISA (ELISA) ug/ml Immunofluorescence (IF) Assay Dependent Western Blot (WB) 1.0 ug/ml Published Applications Western Blot (WB) See 6 publications below Immunoprecipitation (IP) See 2 publications below Miscellaneous PubMed (MISC) See 3 publications below Immunofluorescence (IF) See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Background/Target Information GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

2 Product Images For GST Tag Polyclonal Antibody GST Tag Antibody ( ) Immunoblot of 1 ng sentrin-gst fusion protein using GST polyclonal antibody, rabbit (Product # )

3 GST Tag Antibody in Western Blot (WB) Figure 5 Phosphorylation of SCIMP at Y96 enhances binding to TLR4. ( a ) Pull-down assays using LPSactivated RAW264.7 cell lysates and GST-tagged tyrosine to phenylalanine (Y to F) mutants of SCIMP-T1. SCIMP interactions with either TLR4 or Lyn were assessed by immunoblotting. ( b ) GST pull-down assays were performed using GST-SCIMP mutants and recombinant His-tagged TLR4-TIR domain, and binding was evaluated using immunoblotting against His-TLR4-TIR (upper panel). Quantification of immunoblots was performed using densitometry. Data are combined from two independent experiments (mean+range, lower panel). ( c ) Phosphorylation at Y96 enables SCIMP to bind to TLR4. Titration of fluorescently labelled p-y96- containing (red) or Y96-containing (green) SCIMP-derived peptides (aa ) with TLR4-TIR. Inset shows fluorescence recovery over a longer time course with unlabelled p-y96 peptide. ( d ) The Kd (159 nm and>2.5 mum for p-y96- and Y96-containing peptides, respectively) was determined by curve fit analysis. ( e ) SCIMP phosphorylation at Y96 is required for in vivo TLR4 binding. RAW264.7 cells stably expressing wild type (WT) or Y96F SCIMP were used to assess the agonist-induced SCIMP-TLR4 interaction, by co-immunoprecipitation of SCIMP followed by immunoblotting for TLR4 and SCIMP (left). The level of TLR4 pull-down was quantified relative to SCIMP levels (right) to correct for minor differences in levels of WT versus Y96F SCIMP expression (mean+s.e.m.). Data in a are repr GST Tag Antibody in Western Blot (WB) Published figure using GST Tag polyclonal antibody (Product # ) in Western Blot

4 PubMed References For GST Tag Polyclonal Antibody 6 Western Blot References Not Applicable / 1:1000 Not Applicable / 1: was used in western blot to elucidate the role of SCIMP in Toll-like receptor signaling Nature communications (Jan 2017; 8: null) "SCIMP is a transmembrane non-tir TLR adaptor that promotes proinflammatory cytokine production from macrophages." Author(s):Luo L,Bokil NJ,Wall AA,Kapetanovic R,Lansdaal NM,Marceline F,Burgess BJ,Tong SJ,Guo Z,Alexandrov K,Ross IL, Hibbs ML,Stow JL,Sweet MJ PubMed Article URL: was used in western blot to report the interactome of all dipeptide repeat proteins Cell (Oct 2016; 167: 774) "C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles." Author(s):Lee KH,Zhang P,Kim HJ,Mitrea DM,Sarkar M,Freibaum BD,Cika J,Coughlin M,Messing J,Molliex A,Maxwell BA, Kim NC,Temirov J,Moore J,Kolaitis RM,Shaw TI,Bai B,Peng J,Kriwacki RW,Taylor JP PubMed Article URL: was used in western blot to examiine how Rab8a and PI3Kgamma regulate TLR4 signaling. Nature communications (Jul 2014; 5: null) "Rab8a interacts directly with PI3K to modulate TLR4-driven PI3K and mtor signalling." Author(s):Luo L,Wall AA,Yeo JC,Condon ND,Norwood SJ,Schoenwaelder S,Chen KW,Jackson S,Jenkins BJ,Hartland EL, Schroder K,Collins BM,Sweet MJ,Stow JL PubMed Article URL: was used in western blot to determine the subcellular localisation of wild type and the two most common oncogenic mutants of PI3K. 2 Immunoprecipitation References Bioscience reports (Mar 2014; null: null) "Assessing the subcellular distribution of oncogenic phosphoinositide 3-kinase using microinjection into live cells." Author(s):Layton MJ,Rynkiewicz N,Ivetac I,Horan KA,Mitchell CA,Phillips WA PubMed Article URL: was used in western blot to report and assess the most complete interactome of genes and proteins associated with Alzheimer's disease. Genome research (Mar 2011; 21: 364) "Interactome mapping suggests new mechanistic details underlying Alzheimer's disease." Author(s):Soler-López M,Zanzoni A,Lluís R,Stelzl U,Aloy P PubMed Article URL: Proceedings of the National Academy of Sciences of the United States of America (Dec 1999; 96: 14360) "The polo-box-dependent induction of ectopic septal structures by a mammalian polo kinase, plk, in Saccharomyces cerevisiae." Author(s):Lee KS,Song S,Erikson RL PubMed Article URL: 3 Miscellaneous PubMed References was used in immunoprecipitation to assess the molecular reader and transcriptional co-regulator of H3k4me2/3 called PHF13 elife (May 2016; 5: null) "PHF13 is a molecular reader and transcriptional co-regulator of H3K4me2/3." Author(s):Chung HR,Xu C,Fuchs A,Mund A,Lange M,Staege H,Schubert T,Bian C,Dunkel I,Eberharter A,Regnard C,Klinker H, Meierhofer D,Cozzuto L,Winterpacht A,Di Croce L,Min J,Will H,Kinkley S PubMed Article URL: Blood (Nov 2011; 118: 4841) "HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation." Author(s):Nikolic DS,Lehmann M,Felts R,Garcia E,Blanchet FP,Subramaniam S,Piguet V PubMed Article URL:

5 was used in immunoprecipitation to elucidate the mechanism by which p53 is regulated by RECQL4. Journal of cell science (May 2012; 125: 2509) "RECQL4 is essential for the transport of p53 to mitochondria in normal human cells in the absence of exogenous stress." Author(s):De S,Kumari J,Mudgal R,Modi P,Gupta S,Futami K,Goto H,Lindor NM,Furuichi Y,Mohanty D,Sengupta S PubMed Article URL: was used in western blot to report and assess the most complete interactome of genes and proteins associated with Alzheimer's disease. Genome research (Mar 2011; 21: 364) "Interactome mapping suggests new mechanistic details underlying Alzheimer's disease." Author(s):Soler-López M,Zanzoni A,Lluís R,Stelzl U,Aloy P PubMed Article URL: was used in immunocytochemistry to study E2F-5 subcellular localization in keratinocytes. 1 Immunofluorescence References Mouse / Not Cited The Journal of biological chemistry (Sep 2002; 277: 34471) "Active nuclear import and export pathways regulate E2F-5 subcellular localization." Author(s):Apostolova MD,Ivanova IA,Dagnino C,D'Souza SJ,Dagnino L PubMed Article URL: The Journal of biological chemistry (Mar 2002; 277: 8673) "SH2-B family members differentially regulate JAK family tyrosine kinases." Author(s):O'Brien KB,O'Shea JJ,Carter-Su C PubMed Article URL: