Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005
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1 Deutsche Akkreditierungsstelle GmbH Annex to the Accreditation Certificate D-PL according to DIN EN ISO/IEC 17025:2005 Period of validity: to Holder of certificate: PLANTON GmbH Am Kiel-Kanal 44, Kiel Tests in the fields: molecular biological analysis of specific sequences in food, animal feeding stuffs, commodities, cosmetics, tobacco and tobacco products and other biological materials; molecular biological and immunological analysis of allergenic presence in food, animal feeding stuffs, commodities, cosmetics, tobacco and tobacco products and other biological materials; physical, physico-chemical, sensorical, microbiological, immunological and enzymatic analysis of food and animal feeding stuffs; analysis of mycotoxins and ergot alkaloids in food and animal feeding stuffs; sampling of water from mineral springs and spas; microbiological and selected chemical analysis according drinking water ordinance; sampling of raw and drinking water; forensics test area: forensic genetics (parentage testing, forensic samples) test method: Polymerase Chain Reaction (PCR) test materials: blood, oral mucosa This document is a translation. The definitive version is the original German annex to the accreditation certificate. 1/16
2 The laboratory is permitted within the specified testing fields/areas marked by * without being required to inform and obtain prior approval from the DAkkS *) the free choice of standard or equivalent methods. **) the modification, development and refinement of test methods. ***) to use standard testing methods listed here with different issue dates. The listed test methods are exemplary. The laboratory maintains a current list of all test methods in the flexible scope of accreditiation. Abbreviations used: see last page 1 Molecular biological analysis of specific sequences in food, animal feeding stuffs, commodities, cosmetics, tobacco and tobacco products and other biological materials 1.1 Preparation of samples PLA-SOP0089 DNA-isolation in foods, luxury food, animal feeding stuffs, seeds, raw materials, food additives and other biological materials with PLABEAD system 1.2 Qualitative analysis of genetically modified organisms in food, animal feeding stuffs, commodities, cosmetics, tobacco and tobacco products and other biological materials by Polymerase Chain Reaction (PCR) ** ASU-L including correction from ASU-L Analysis of foods - screening method for detection of genetically modified DNA-sequences in foods with detection of DNAsequences commonly used in genetically modified organisms (modification: additional application in commodities, cosmetics, tobacco and tobacco products and other biological materials) Analysis of foods - detection of genetic modifications in maize (Zea mays L.) with PCR (Polymerase Chain Reaction) and restriction analysis or hybridization of the PCR-product (modification: additional application in commodities, cosmetics, tobacco and tobacco products and other biological materials) Period of validity: to Translation - 2/16
3 ASU-L PLA-SOP0006 PLA-SOP0007 PLA-SOP0008 PLA-SOP0009 PLA-SOP0010 PLA-SOP0011 PLA-SOP0013 PLA-SOP0017 PLA-SOP0019 PLA-SOP0020 PLA-SOP0021 Analysis of foods - detection of a genetic modification in soybeans by amplification of modified DNA-sequences with PCR (Polymerase Chain Reaction) and hybridization of the PCRproduct with a DNA-probe (modification: additional application in commodities, cosmetics, tobacco and tobacco products and other biological materials) Qualitative analysis of RoundupReady-oil seed rape Qualitative analysis of LibertyLink-oil seed rape Qualitative analysis of SeedLink-oil seed rape Qualitative analysis of Laurate-oil seed rape Qualitative analysis of Oxy235-oil seed rape Qualitative analysis of Bt176-corn Qualitative analysis of MON810-corn Qualitative analysis of GA21-corn Qualitative analysis of RoundupReady (GTS40-3-2) soy Qualitative analysis of CaMV35S-Promotor Qualitative analysis of Nopalin-Terminator (T-NOS) Period of validity: to Translation - 3/16
4 PLA-SOP0092 PLA-SOP0117 PLA-SOP0144 PLA-SOP0146 PLA-SOP0150 PLA-SOP0156 Qualitative analysis of FP967 (CDC Triffid)-linseed Qualitative analysis of FMV-Promotor Qualitative analysis of H7-1 sugar beet Qualitative analysis of Amflora EH92 potato Qualitative analysis of Bt63 rice Qualitative analysis of MIR604 corn 1.3 Qualitative species identification by PCR ** PLA-SOP0003 PLA-SOP0004 PLA-SOP0005 PLA-SOP102 PLA-SOP103 PLA-SOP104 PLA-SOP105 Species identification of oil seed rape Species identification of corn Species identification of soy Species identification of chicken Species identification of cow Species identification of pig Species identification of shark Period of validity: to Translation - 4/16
5 PLA-SOP106 PLA-SOP PLA-SOP Species identification of turkey Species identification of mustard Analysis of foods - PCR-based and cultural detection of Salmonella ssp. 1.4 Quantitative analysis of genetically modified organisms by real time PCR ** PLA-SOP0026 PLA-SOP0027 PLA-SOP0028 PLA-SOP0029 PLA-SOP0030 PLA-SOP0033 PLA-SOP0034 PLA-SOP0037 PLA-SOP0039 PLA-SOP0140 Quantitative analysis of RoundupReady oil seed rape Quantitative analysis of LibertyLink oil seed rape Quantitative analysis of SeedLink oil seed rape Quantitative analysis of Laurate oil seed rape Quantitative analysis of OXY235 oil seed rape Quantitative analysis of MON810 corn Quantitative analysis of MON863 corn Quantitative analysis of GA21 corn Quantitative analysis of RoundupReady (GTS40-3-2) soy Quantitative analysis of RoundupReady II soy Period of validity: to Translation - 5/16
6 PLA-SOP0143 PLA-SOP0145 PLA-SOP0149 PLA-SOP0155 Quantitative analysis of H7-1 sugar beet Quantitative analysis of Amflora EH92 potato Quantitative analysis of Bt63 rice Quantitative analysis of MIR604 corn 1.5 Quantitative species identification by real time PCR ** PLA-SOP0023 PLA-SOP0024 PLA-SOP0025 PLA-SOP0080 PLA-SOP0142 PLA-SOP0151 PLA-SOP0152 PLA-SOP0233 Oilseed Rape (species-specific), quantitative Corn (species-specific), quantitative Soy (species-specific), quantitative Sugar beet (species-specific), quantitative Rice (species-specific), quantitative Cotton (species-specific), quantitative Potato (species-specific), quantitative Horse (species-specific), quantitative Period of validity: to Translation - 6/16
7 2 Detection of mycotoxins and ergot alkaloids in food and animal feeding stuffs 2.1 Detection of mycotoxins and ergot alkaloids by HPLC with standard detector** DIN EN ISO DIN EN DIN EN DIN EN PLA-SOP0315 PLA-SOP0338 Milk and milk powder- Determination of aflatoxin M1 content - cleanup by immunoaffinity-chromatography and determination by High Performance Liquid Chromatography Determination of aflatoxin B 1 and the total content of aflatoxin B 1, B 2, G 1 and G 2 in cereals, shell-fruits and derived products - High Performance Liquid Chromatographic (HPLC) method with post column derivatization and immunoaffinity-column clean-up Food - Detection of Aflatoxin B 1 and the total content of aflatoxin B 1, B 2, G 1 und G 2 in hazelnuts, peanuts, pistachio, fig and paprica powder - High Performance Liquid Chromatographic (HPLC) method with post column derivatization and immunoaffinity-column clean-up Food - Determination of ochratoxin A in barley and roasted coffee HPLC-method with immunoaffinity-column clean-up Quantitative detection of T2- and HT2- toxin in food and animal feeding stuffs Quantitative detection of ergot alkaloids in food and animal feeding stuffs 2.2 Quantitative detection of mycotoxins with Enzyme-linked Immunosorbent Assay (ELISA) by using testkits * PLA-SOP PLA-SOP PLA-SOP Quantitative detection of total aflatoxin Quantitative detection of deoxynivalenol (DON) Quantitative detection of zearalenon (ZEA) Period of validity: to Translation - 7/16
8 PLA-SOP PLA-SOP PLA-SOP Quantitative detection of ochratoxin A (OTA) Quantitative detection of fumonisins Quantitative detection of T2- toxin 3 Detection of allergens in food, animal feeding stuffs, commodities, cosmetics, tobacco and tobacco products and other biological materials 3.1 Quantitative detection of allergens with ELISA by using testkits * R-Biopharm, RIDASCREEN, Gliadin, R7001 PLA-SOP PLA-SOP PLA-SOP PLA-SOP PLA-SOP PLA-SOP Quantitative detection of gliadin in food and animal feeding stuffs (modification: additional application in commodities, cosmetics, tobacco and tobacco products and other biological materials) Quantitative detection of fish-allergen Quantitative detection of gliadin Quantitative detection of peanut protein Quantitative detection of bovine casein Quantitative detection of soy protein Quantitative detection of mustard seeds Period of validity: to Translation - 8/16
9 3.2 Detection of allergens by real time PCR Preparation of samples PLA-SOP0089 PLA-SOP PLA-SOP PLA-SOP PLA-SOP PLA-SOP DNA-isolation in foods, luxury food, animal feeding stuffs, seeds, raw materials, food additives and other biological materials with PLABEAD-system Quantitative species detection of celery Quantitative species detection of mustard Quantitative species detection of peanut Quantitative species detection of hazelnut Quantitative species detection of paranut PLA-SOP Quantitative species detection of walnut 3.3 Quantitative detection of allergens by enzymatic assay ** PLA-SOP PLA-SOP PLA-SOP Quantitative species detection of galactose, enzymatic assay Quantitative species detection of lactose, enzymatic assay Quantitative species detection of sulfite, enzymatic assay Period of validity: to Translation - 9/16
10 4 Determination of plant protection products (pesticide residues) and other contaminants in food and animal feeding stuffs 4.1 Determination of plant protection products (pesticide residues) and other contaminants by Liquid Chromatography LC-MS/MS ** ASU L ASU L ASU L PLA-SOP Examination of foods - Modular Multi Method for the determination of pesticides in food and animal feeding stuffs (DFG method S 19) Examination of foods of plant origin - Determination of the growth-regulating ingredients chlormequate and mepiquate by LC-MS/MS (corresponding to DIN EN 15055; 2006-August) Examination of foods -Multi Method for the determination of pesticide residues in foods of plant origin using GC-MS(/MS) or LC-MS/MS after acetonitrile extraction/dispersion and clean-up by dispersive SPE (QuEChERS) Quantitative detection of glyphosate and AMPA by LC-MS/MS in food and animal feeding stuffs 4.2 Determination of plant protection products (pesticide residues) and other contaminants by gas chromatography (GC) with standard detectors (MS- and MS/MS-detector, HS-GC) * ASU L ASU L / ASU L / mit Berichtigung Examination of foods - Modular Multi Method of the determination of pesticides in food and animal feeding stuffs (DFG method S 19) Examination of foods - Determination of bromide residues in non-fatty foods - part 1: determination of total bromide as inorganic bromide (corresponding to DIN EN 13191; 2000-October) Examination of foods - Non-fatty foods - Determination of dithiocarbamate and thiuram disulfide residues - Part 2: Gas chromatographic method (corresponding to DIN EN ; 1998-December) Period of validity: to Translation - 10/16
11 ASU L Examination of foods -Multi Method for the determination of pesticide residues in foods of plant origin using GC-MS(/MS) or LC-MS/MS after acetonitrile extraction/dispersion and clean-up by dispersive SPE (QuEChERS) 5 Microbiological tests in food and animal feeding stuffs 5.1 Cultural bacteriological tests (e.g. spatula method, method with poured plates, MPN- methods) * ISO ASU L ASU L ASU L / ASU L / PLA-SOP Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions Examination of foods - Horizontal method for the detection of Salmonella spp. in food (corresponding to DIN EN ISO 6579; 2007-October) Examination of foods - Method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Part 1: Technique using Baird-Parker agar medium (corresponding to DIN EN ISO ; 2003-December) Examination of foods - Horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli in food - part 2: colony enumeration method by 5-Brom-4-Chlor-3-Indol-β-D- Glucuronide (corresponding to DIN ISO ; 2009-December) Examination of foods - Horizontal method for the detection and enumeration of enterobacteriaceae - part 2: colony enumeration method (corresponding to DIN ISO ; 2009-December) Examination of foods - PCR-based and cultural detection of Salmonella ssp. Period of validity: to Translation - 11/16
12 6 Sensorical, physical and physico-chemical analysis in food and animal feeding stuffs 6.1 Sensorical tests ASU L Examination of foods - sensorical test, simple descriptory analysis 6.2 Determination of parameters by potentiometry ASU L Determination of ph-value in meat and meat products 6.3 Gravimetric analysis of food and animal feeding stuffs ASU L VO (EG) 152/ 2009, III, A VDLUFA VI, C Determination of dry matter in meat and meat products Determination of moisture content in animal feeding stuffs Dry matter (water content) - sea sand method 7 Analysis according drinking water ordinance 2001 (Trinkwasserverordnung) *** Method DIN EN ISO (A 4) DIN ISO (A 14) DIN EN ISO (A 21) DIN EN ISO (K 19) DIN A Titel Water quality - Sampling - Part 1: Guidance on the design of sampling programmes and sampling techniques Water quality - Sampling - Part 5: Guidance on sampling of drinking water from treatment works and piped distribution systems Water quality - Sampling - Part 3: Preservation and handling of water samples Water quality - Sampling for microbiological analysis Sampling of water from mineral springs and spas Period of validity: to Translation - 12/16
13 Annex 1: MICROBIOLOGICAL PARAMETER PART I: General Requirements for drinking water No. Parameter Method 1 Escherichia coli (E. coli) DIN EN ISO (K 12) mit Colilert -18/Quanti-Tray 2 Enterokokken DIN EN ISO (K 15) PART II: Requirements for drinking water, that is used for distribution in closed containments No. Parameter Method 1 Escherichia coli (E. coli) DIN EN ISO (K 12) Colilert -18/Quanti-Tray 2 Enterokokken DIN EN ISO (K 15) Pseudomonas aeruginosa DIN EN ISO (K 11) Annex 2: CHEMICAL PARAMETER PART I: Chemical parameter that generally do not increase in concentration within the drinking water distribution network and its installations. No. Parameter Method 1 Acrylamid not performed 2 Benzol not performed 3 Bor not performed 4 Bromat not performed 5 Chrom not performed 6 Cyanid not performed 7 1,2-Dichlorethan not performed 8 Fluorid not performed 9 Nitrat not performed 10 Pflanzenschutzmittel- Wirkstoffe und Biozidprodukt- DIN EN ISO (F 12) Wirkstoffe 11 Pflanzenschutzmittel- Wirkstoffe und Biozidprodukt- DIN EN ISO (F 6) Wirkstoffe insgesamt 12 Quecksilber not performed 13 Selen not performed Period of validity: to Translation - 13/16
14 No. Parameter Method 14 Tetrachlorethen und Trichlorethen not performed 15 Uran not performed PART II: Chemical parameter, parameter that may increase in concentration within the drinking water distribution network and its installations. Not performed ANNEX 3: INDICATION PARAMETERS PART I: General indication parameters No. Parameter Method 1 Aluminium not performed 2 Ammonium not performed 3 Chlorid not performed 4 Clostridium perfringens (einschließlich Sporen) TrinkwV 2001 Anl. 5 I e) 5 Coliforme Bakterien DIN EN ISO (K12) Colilert -18/Quanti-Tray 6 Eisen not performed 7 Färbung (spektraler Absorptionskoeffizient Hg 436 nm) not performed 8 Geruch not performed 9 Geschmack not performed 10 Koloniezahl bei 22 C 11 Koloniezahl bei 36 C 12 Elektrische Leitfähigkeit not performed 13 Mangan not performed 14 Natrium not performed 15 Organisch gebundener Kohlenstoff (TOC) DIN EN ISO 6222 (K 5) TrinkwV 2001 Anl. 5 I d) bb) DIN EN ISO 6222 (K 5) TrinkwV 2001 Anl. 5 I d) bb) not performed 16 Oxidierbarkeit not performed Period of validity: to Translation - 14/16
15 No. Parameter Method 17 Sulfat not performed 18 Trübung not performed 19 Wasserstoffionen-Konzentration not performed 20 Calcitlösekapazität not performed 21 Tritium not performed 22 Gesamtrichtdosis not performed Part II: Specific requirements for drinking water in facilities of drinking water installations. Parameter Legionella spec. Method DIN EN ISO (K 22) UBA recommendation The accreditation is not a replacement for the recognition or authorization-procedure by the competent authority according to article 15 (4) German drinking water ordinance 8 Test field forensics Test area: forensic genetics (parentage testing, forensic samples) Test method: Polymerase Chain Reaction (PCR) Test parameter Test material Test method Genotype for determination of parentage Genotype for determination of forensic samples Human DNA from: - oral mucosa - blood Human DNA from: - oral mucosa - blood STR-analysis: PCR with flexible PCR reagents followed by electrophoresis and allelic assignment of PCR products STR-analysis: PCR with flexible PCR reagents followed by electrophoresis and allelic assignment of PCR products Period of validity: to Translation - 15/16
16 Abbreviations used: ASU DIN DNA EN GC HPLC HS IEC ISO MS PCR PLA-SOP STR VDLUFA VDLUFA VI VO (EG) Official collection of test methods according to 64 para. 1 German Food and Feed Code (LFGB) (Amtliche Sammlung von Untersuchungsverfahren) German Institute of Standardization (Deutsches Institut für Normung) Deoxyribonucleic acid (Desoxyribonukleinsäure) European Norm Gas Chromatography High Performance Liquid Chromatography Headspace International Electrotechnical Commission, Genf (Internationale Elektrotechnische Kommission) International Organisation for Standardisation Mass Spectroscopy Polymerase Chain Reaction In-house procedure of PLANTON company Short Tandem Repeat Association of German Agricultural Analytic and Research Institutes (Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten) Association of German Agricultural Analytic and Research Institutes, method book IV, Milk (Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten, Methodenbuch VI, Milch) Regulation (European Community) Period of validity: to Translation - 16/16
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