Protein Cat. No. Sizes
|
|
|
- Melvin Booth
- 6 years ago
- Views:
Transcription
1 Writers - Proteins & Antibodies EPIGENETIC WRITERS DNA Methyltransferases (DNMTs) Histone Acetyltransferases (HATs) Kinases Poly(ADP-ribose) Polymerases(PARPs) Protein Arginine Methyltransferases (PRMTs) Protein Lysine Methyltransferases (PKMTs) Ubiquitin E2 Conjugases and Ubiquitin E3 Ligases DNA METHYLTRANSFERASES (DNMTs) 155 S. Milipitas Blvd, Milipitas, CA T: F: Toll Free: (US Only) Epigenetic Writers catalyze the addition of chemical groups like the acetyl, methyl, phosphate or ubiquitinyl groups to DNA or Histone to create epigenetic marks. Such marks are crucial for gene expression and silencing. Many of the epigenetic writers present as targets in therapeutic areas of cancer, diabetes and autoimmune diseases. BioVision offers proteins and antibodies for a wide range of such therapeutic and many more targets. These enzymes catalyze the addition of methyl groups onto the cytosine residue of CpG dinucleotides on DNA to induce transcriptional activation/repression. Methylation anomalies have been shown to play a direct causal role in tumorigenesis and genetic disease BioVision s Key DNMT Recombinant Proteins DNA Methyltransferase 3L ( aa), Human recombinant μg S-Adenosyl-L-methionine disulfate tosylate mg, 100 mg, 500 mg BioVision s Key DNMT Antibodies Anti-5-Hydroxymethylcytosine Antibody A μg Anti-5-Methylcytosine Antibody A μg ASH2 polyclonal antibody μl CBX2 polyclonal antibody μl DMAP1 Antibody μg DNA Methyltransferase 1 (Clone 60B1220.1) Antibody μg DNA Methyltransferase 2 (Clone 102B1259.2) Antibody μg DNA Methyltransferase 2 Antibody μg DNA Methyltransferase 3a (Clone 64B1446) Antibody μg DNA Methyltransferase 3a (Clone 64B814.1) Antibody μg DNA Methyltransferase 3b (Clone 52A1018) Antibody μg
2 DNMT1 Antibody μg DNMT2 Antibody μg DNMT2 polyclonal antibody μl DNMT3a Antibody μg DNMT3A polyclonal antibody μg DNMT3b Antibody μg EHMT1 polyclonal antibody μl EzH2 polyclonal antibody μg MBD1 (Clone 100B272.1) Antibody μg MBD1 polyclonal antibody μg MBD2/3 (Clone 106B691) Antibody μg MeCP2 (aa 11-25; aa ) Antibody μg MeCP2 Antibody μg MeCP2 polyclonal antibody μg S- Adenosylhomocysteine Antibody (Clone # ) µl S- Adenosylmethionine Antibody µl S- Adenosylmethionine Antibody (Clone # ) µl S- Adenosylmethionine Antibody (Clone # 118-6) µl S- Adenosylmethionine Antibody (Clone # 84-19) µl S- Adenosylmethionine Antibody (Clone # 84-3) µl Set9 polyclonal antibody μg Setd1a polyclonal antibody μl Setd1b polyclonal antibody μl SETD8 polyclonal antibody μg TAF1 polyclonal antibody μl WDR5 polyclonal antibody μl HISTONE ACETYLTRANSFERASES (HATs) These enzymes catalyze the acetylation of lysine residues on histone proteins. In general, this acetylation leads to an increase in gene expression. HATs can also acetylate non-histone proteins, such as transcription factors and nuclear receptors to facilitate gene expression. Developmental aberrations in mice and certain human cancers are associated with HAT mutations. BioVision s Key HAT Recombinant Proteins HeLa Nuclear Extract mg, 100 μg PCAF bromodomain ( aa), human recombinant μg PCAF bromodomain ( aa), human recombinant μg, 100 μg PCAF, mouse recombinant μg BioVision s Key HAT Antibodies Acetyl Lysine (Biotin) Antibody μg Acetyl-Lysine Monoclonal Antibody A µg HAT-1 Antibody μg HAT-2 Antibody μg HAT-3 Antibody μg KAT8 Antibody μg NCOA1 Antibody μg 2
3 RBBP4 Antibody μg TIP60 Antibody μg TIP60 Antibody μg KINASES Kinases play an important role in epigenetic regulation through PARP activation. BioVision s Key Kinase Recombinant Proteins Active Pim μg Active PIM μg Active PKCepsilon μg BioVision s Key Kinase Antibodies ATM Antibody μg ATR Antibody μg PIM1 Antibody 3787R 100 μg PKC Antibody μg POLY (ADP-RIBOSE) POLYMERASES (PARPs) These enzymes mediate the Poly ADP-ribosylation of proteins. They are mainly involved in DNA repair and cell death. BioVision s Key PARP Recombinant Proteins PARP-1, human recombinant μg BioVision s Key PARP Antibodies PARP (Cleaved) Antibody μg PARP (Cleaved) Antibody μg BioVision s Key PARP Antibodies PARP (Cleaved) Antibody μg PARP Antibody μg PARP Antibody (Clone 10H) μg PARP Antibody (Clone 7A10) μg PROTEIN ARGININE METHYLTRANSFERASE (PRMTs) These enzymes mediate the generation of monomethylated or dimethylated arginine residues. The symmetry of the methyl groups added to arginine residues determines the biological effect of the epigenetic modification. Asymmetric dimethylation is linked to gene activation while symmetric dimethylation is associated with gene repression. BioVision s Key PRMT Recombinant Proteins PRMT1, human recombinant (Active) μg, 50 μg, 1mg PRMT1, mouse recombinant (Active) μg, 1mg 3
4 BioVision s Key PRMT Antibodies CARM1 Antibody 3734R 100 μg PRMT1 Antibody μg PRMT-5 Antibody μg PRMT6 Antibody μg PRMT7 Antibody μg PROTEIN LYSINE METHYLTRANSFERASE (PKMTs) These enzymes catalyze the transfer of a methyl group from the co-factor S-adenosyl methionine (SAM) onto a lysine side chain on the exposed histone tail. Proteins differentially interact with these methylated histones based on the number of methyl residues added. BioVision s Key PKMT Recombinant Proteins Ash2L ( aa), Human recombinant μg DOT1L (2-416 aa), Human recombinant μg EED (1-441aa), Human recombinant μg Nicotinamide N-Methyltransferase, Human Recombinant (hnnmt) μg, 100 μg, 1mg RIZ1 (2-200 aa) (GST-tagged), Human recombinant μg BioVision s Key PKMT Antibodies EHMT2 Antibody μg EZH1 Antibody μg EZH2 Antibody μg Methyl Lysine (Biotin) Antibody μg NNMT Antibody µl, 100 µl SETD3 Antibody μg SETDB1 Antibody μg Swi6 Antibody μg UBIQUITIN E2 CONJUGASES and UBIQUITIN E3 LIGASES Lysine residues on histone proteins H2A and H2B can undergo monoubiquitination through the concerted actions of E2 ubiquitin conjugases and E3 ubiquitin ligases which forms a very critical epigenetic mark. Depending on the location of ubiquitination, transcription can either be activated or repressed. Human recombinant UBE2D2 (UbcH5b) nmol Human recombinant UBE2D nmol Human recombinant UBE2E2 (UbcH8) nmol Human recombinant UBE2E3 (UbcH9) nmol Human recombinant UBE2H (UbcH2) nmol Human recombinant UBE2H (UbcH2), HIS6SUMO nmol Human recombinant UBE2K nmol 4
5 Human recombinant UBE2L nmol Human recombinant UBE2R1 (CDC34) nmol Human recombinant UBE2R2 (Ubc3B) nmol Human recombinant Ubiquitin Activating Enzyme E μg UbcH1, human recombinant (GST-tag) μg, 100 μg UbcH2, human recombinant (His-tag) μg UbcH3, human recombinant (His-tag) μg UbcH5a, human recombinant (His-tag) μg UbcH5b, human recombinant (His-tag) μg UbcH5c, human recombinant (His-tag) μg Yeast recombinant Ubc13 (UBE2N) nmol BioVision s Key Epigenetic Ubiquitination related Antibodies USP7 Antibody μg RELATED PRODUCTS Category Product Type Histones Core Histones, Linker Histones Reader Domains Bromodomains, Tudor Domains, MBT Domains Eraser Enzymes HDACs, HDMs, HIF, PTPs, SIRTs, and more Please visit for a comprehensive overview on our products. 5
Lecture 21: Epigenetics Nurture or Nature? Chromatin DNA methylation Histone Code Twin study X-chromosome inactivation Environemnt and epigenetics
Lecture 21: Epigenetics Nurture or Nature? Chromatin DNA methylation Histone Code Twin study X-chromosome inactivation Environemnt and epigenetics Epigenetics represents the science for the studying heritable
Histone H3 Acetylation Antibody Panel Pack II Base Catalog # C K23A Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody 25 µg 4 C 20 C
Histone H3 Acetylation Antibody Panel Pack II Base Catalog # PACK CONTENTS Component Size Shipping Temperature Upon Receipt Checklist 3K23A Histone H3K23ac (Acetyl H3K23) Polyclonal Antibody 25 µg 4 C
Chromatin and Transcription
Chromatin and Transcription Chromatin Structure Chromatin Represses Transcription Nucleosome Positioning Histone Acetylation Chromatin Remodeling Histone Methylation CHIP Analysis Chromatin and Elongation
TOOLS FOR DRUG DISCOVERY
TOOLS FOR DRUG DISCOVERY targeting epigenetics and transcription Epigenetics Gene Regulation mirna www.activemotif.com DRUG DISCOVERY TOOLBOX assays, reagents and services to accelerate your research Target
EPIGENTEK. EpiQuik In Situ Histone H3-K27 Tri-Methylation Assay Kit. Base Catalog # P-3014T PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik In Situ Histone H3-K27 Tri-Methylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik In Situ Histone H3-K27 Tri-Methylation Assay Kit is suitable for specifically
Post-translational modifications of histones: encoding or patterning? Histones are subject to an enormous number of posttranslational
R546 Primer Histones and histone modifications Craig L. Peterson and Marc-André Laniel Imagine trying to stuff about 10,000 miles of spaghetti inside a basketball. Then, if that was not difficult enough,
EUKARYOTIC GENE CONTROL
EUKARYOTIC GENE CONTROL THE BIG QUESTIONS How are genes turned on and off? How do cells with the same DNA/ genes differentiate to perform completely different and specialized functions? GENE EXPRESSION
ab Histone H3 (tri-methyl K27) Quantification Kit (Fluorometric)
ab115073 Histone H3 (tri-methyl K27) Quantification Kit (Fluorometric) Instructions for Use For the measurement of global H3K27me 3 This product is for research use only and is not intended for diagnostic
Control of Eukaryotic Genes. AP Biology
Control of Eukaryotic Genes The BIG Questions How are genes turned on & off in eukaryotes? How do cells with the same genes differentiate to perform completely different, specialized functions? Evolution
Chapter 13. The Nucleus. The nucleus is the hallmark of eukaryotic cells; the very term eukaryotic means having a "true nucleus".
Chapter 13 The Nucleus The nucleus is the hallmark of eukaryotic cells; the very term eukaryotic means having a "true nucleus". Fig.13.1. The EM of the Nucleus of a Eukaryotic Cell 13.1. The Nuclear Envelope
Control of Eukaryotic Gene Expression (Learning Objectives)
Control of Eukaryotic Gene Expression (Learning Objectives) 1. Compare and contrast chromatin and chromosome: composition, proteins involved and level of packing. Explain the structure and function of
Total Histone H3 Acetylation Detection Fast Kit (Fluorometric)
Total Histone H3 Acetylation Detection Fast Kit (Fluorometric) Catalog Number KA1539 48 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use...
Gene Regulation in Eukaryotes. Dr. Syahril Abdullah Medical Genetics Laboratory
Gene Regulation in Eukaryotes Dr. Syahril Abdullah Medical Genetics Laboratory syahril@medic.upm.edu.my Lecture Outline 1. The Genome 2. Overview of Gene Control 3. Cellular Differentiation in Higher Eukaryotes
Histone Analysis. Histone Analysis
Histone Analysis Histone Analysis innovative tools designed to help unravel the histone code Histone Antibodies Histone Purification Recombinant Histones Histone Modifying Enzymes Histone Modification
Chromatin Structure and its Effects on Transcription
Chromatin Structure and its Effects on Transcription Epigenetics 2014 by Nigel Atkinson The University of Texas at Austin From Weaver 4th edition and Armstrong 1st edition What is the point? DNA is not
Control of Eukaryotic Genes. AP Biology
Control of Eukaryotic Genes The BIG Questions How are genes turned on & off in eukaryotes? How do cells with the same genes differentiate to perform completely different, specialized functions? Evolution
Epigenetics. Medical studies in English, Lecture # 12,
Epigenetics Medical studies in English, 2018. Lecture # 12, Epigenetics Regulation of gene activity in eukaryotes Correlation of chromatin structure with transcription stably heritable phenotype resulting
EpiSeeker Histone Methyltransferase H3 (K4) Activity Quantification Assay Kit
ab113452 EpiSeeker Histone Methyltransferase H3 (K4) Activity Quantification Assay Kit Instructions for Use For the measurement of activity/inhibition of H3- K4 specific histone methyltransferases using
Differential Gene Expression
Biology 4361 Developmental Biology Differential Gene Expression June 19, 2008 Differential Gene Expression Overview Chromatin structure Gene anatomy RNA processing and protein production Initiating transcription:
Chromatin. Structure and modification of chromatin. Chromatin domains
Chromatin Structure and modification of chromatin Chromatin domains 2 DNA consensus 5 3 3 DNA DNA 4 RNA 5 ss RNA forms secondary structures with ds hairpins ds forms 6 of nucleic acids Form coiling bp/turn
Plant Molecular and Cellular Biology Lecture 9: Nuclear Genome Organization: Chromosome Structure, Chromatin, DNA Packaging, Mitosis Gary Peter
Plant Molecular and Cellular Biology Lecture 9: Nuclear Genome Organization: Chromosome Structure, Chromatin, DNA Packaging, Mitosis Gary Peter 9/16/2008 1 Learning Objectives 1. List and explain how DNA
ab Histone H3 (tri-methyl K9) Quantification Kit
ab115064 Histone H3 (tri-methyl K9) Quantification Kit Instructions for Use For the measurement of global histone H3K9 tri-methylation. This product is for research use only and is not intended for diagnostic
Value Correct Answer Feedback. Student Response. A. Dicer enzyme. complex. C. the Dicer-RISC complex D. none of the above
1 RNA mediated interference is a post-transcriptional gene silencing mechanism Which component of the RNAi pathway have been implicated in cleavage of the target mrna? A Dicer enzyme B the RISC-siRNA complex
AP Biology Gene Expression/Biotechnology REVIEW
AP Biology Gene Expression/Biotechnology REVIEW Multiple Choice Identify the choice that best completes the statement or answers the question. 1. Gene expression can be a. regulated before transcription.
Unit IX Problem 3 Genetics: Basic Concepts in Molecular Biology
Unit IX Problem 3 Genetics: Basic Concepts in Molecular Biology - The central dogma (principle) of molecular biology: Information from DNA are transcribed to mrna which will be further translated to synthesize
Differential Gene Expression
Biology 4361 - Developmental Biology Differential Gene Expression June 18, 2009 Differential Gene Expression Overview Chromatin structure Gene anatomy RNA processing and protein production Initiating transcription:
Complete Sample to Analysis Solutions for DNA Methylation Discovery using Next Generation Sequencing
Complete Sample to Analysis Solutions for DNA Methylation Discovery using Next Generation Sequencing SureSelect Human/Mouse Methyl-Seq Kyeong Jeong PhD February 5, 2013 CAG EMEAI DGG/GSD/GFO Agilent Restricted
Control of Eukaryotic Genes
Control of Eukaryotic Genes 2007-2008 The BIG Questions How are genes turned on & off in eukaryotes? How do cells with the same genes differentiate to perform completely different, specialized functions?
DNA. Is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses.
Is a molecule that encodes the genetic instructions used in the development and functioning of all known living organisms and many viruses. Genetic information is encoded as a sequence of nucleotides (guanine,
ANTIBODY PERFORMANCE COMPARISON. Histone Modifications
ANTIBODY PERFORMANCE COMPARISON Histone Modifications COVER IMAGE: Methylation of cytosine bases in regions called CpG islands is a hallmark of transcriptionally repressed heterochromatin. These methylated
Chapter 18: Regulation of Gene Expression. 1. Gene Regulation in Bacteria 2. Gene Regulation in Eukaryotes 3. Gene Regulation & Cancer
Chapter 18: Regulation of Gene Expression 1. Gene Regulation in Bacteria 2. Gene Regulation in Eukaryotes 3. Gene Regulation & Cancer Gene Regulation Gene regulation refers to all aspects of controlling
Genetics Biology 331 Exam 3B Spring 2015
Genetics Biology 331 Exam 3B Spring 2015 MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) DNA methylation may be a significant mode of genetic regulation
3. How Genomes Function
3. How Genomes Function In order for the cell to utilize the biological information contained within its genome, groups of genes, each gene representing a single unit of information, have to be expressed
Modern Epigenomics. Histone Code
Modern Epigenomics Histone Code Ting Wang Department of Genetics Center for Genome Sciences and Systems Biology Washington University Dragon Star 2012 Changchun, China July 2, 2012 DNA methylation + Histone
Differential Gene Expression
Biology 4361 Developmental Biology Differential Gene Expression September 28, 2006 Chromatin Structure ~140 bp ~60 bp Transcriptional Regulation: 1. Packing prevents access CH 3 2. Acetylation ( C O )
Molecular Genetics II - Genetic Engineering Course (Supplementary notes)
1 von 12 21.02.2015 15:13 Molecular Genetics II - Genetic Engineering Course (Supplementary notes) Figures showing examples of cdna synthesis (currently 11 figures) cdna is a DNA copy synthesized from
Chapter 11: Regulation of Gene Expression
Chapter Review 1. It has long been known that there is probably a genetic link for alcoholism. Researchers studying rats have begun to elucidate this link. Briefly describe the genetic mechanism found
Exam 2 BIO200, Winter 2012
Exam 2 BIO200, Winter 2012 Name: Multiple Choice Questions: Circle the one best answer for each question. (2 points each) 1. The 5 cap structure is often described as a backwards G. What makes this nucleotide
Automated, High Throughput, HTRF -Based Detection of Histone Methyltransferase and Demethylase Enzyme Activity
A p p l i c a t i o n N o t e Automated, High Throughput, HTRF -Based Detection of Histone Methyltransferase and Demethylase Enzyme Activity Brad Larson and Peter Banks, Applications Department, BioTek
Assessment of HDAC1 Inhibition Using an Automated, Bioluminescent Histone Deacetylase I/II Assay
A p p l i c a t i o n N o t e Assessment of HDAC1 Inhibition Using an Automated, Bioluminescent Histone Deacetylase I/II Assay Brad Larson, BioTek Instruments, Inc., Winooski, VT Tracy Worzella, Promega
ab Methylated DNA Quantification Kit (Colorimetric)
ab117128 Methylated DNA Quantification Kit (Colorimetric) Instructions for Use For the measurement of global DNA methylation status using DNA isolated from any species such as mammalians, plants, fungi,
EPIGENTEK. EpiQuik Chromatin Immunoprecipitation Kit. Base Catalog # P-2002 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Chromatin Immunoprecipitation Kit Base Catalog # P-2002 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Chromatin Immunoprecipitation Kit is suitable for combining the specificity of
RNA Structure and the Versatility of RNA. Mitesh Shrestha
RNA Structure and the Versatility of RNA Mitesh Shrestha Ribonucleic Acid (RNA) Nitrogenous Bases (Adenine, Uracil, Guanine, Cytosine) Ribose Sugar Ribonucleic Acid (RNA) Phosphate Group RNA world Hypothesis
What we ll do today. Types of stem cells. Do engineered ips and ES cells have. What genes are special in stem cells?
Do engineered ips and ES cells have similar molecular signatures? What we ll do today Research questions in stem cell biology Comparing expression and epigenetics in stem cells asuring gene expression
Department of Biochemistry and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907, USA
A charge-based interaction between histone H4 and Dot1 is required for H3K79 methylation and telomere silencing: identification of a new trans-histone pathway Ian M. Fingerman, Hui-Chun Li, and Scott D.
Differential Gene Expression
IBS 8102 Cell, Molecular, and Developmental Biology Differential Gene Expression January 22, 2008 Differential Gene Expression Chromatin structure Gene anatomy Gene sequences Control of gene transcription
Do engineered ips and ES cells have similar molecular signatures?
Do engineered ips and ES cells have similar molecular signatures? Comparing expression and epigenetics in stem cells George Bell, Ph.D. Bioinformatics and Research Computing 2012 Spring Lecture Series
Lecture Overview. Overview of the Genetic Information. Marieb s Human Anatomy and Physiology. Chapter 3 DNA & RNA Protein Synthesis Lecture 6
Marieb s Human Anatomy and Physiology Marieb Hoehn Chapter 3 DNA & RNA Protein Synthesis Lecture 6 Lecture Overview The Genetic Information Structure of DNA/RNA DNA Replication Overview of protein synthesis
Instructions For Research Use Only. Not For Use In Diagnostic Procedures
Instructions For Research Use Only. Not For Use In Diagnostic Procedures HT Universal Chemiluminescent PARP Assay Kit with Histone-coated Strip Wells 96 Tests Cat# 4676-096-K Chemiluminescent assay kit
2. Outline the levels of DNA packing in the eukaryotic nucleus below next to the diagram provided.
AP Biology Reading Packet 6- Molecular Genetics Part 2 Name Chapter 19: Eukaryotic Genomes 1. Define the following terms: a. Euchromatin b. Heterochromatin c. Nucleosome 2. Outline the levels of DNA packing
ab Ubiquitylation Assay Kit (HeLa lysate-based)
ab139471 Ubiquitylation Assay Kit (HeLa lysate-based) Instructions for Use For the generation of ubiquitin-conjugated lysate proteins This product is for research use only and is not intended for diagnostic
32 Gene regulation in Eukaryotes Lecture Outline 11/28/05. Gene Regulation in Prokaryotes and Eukarykotes
3 Gene regulation in Eukaryotes Lecture Outline /8/05 Gene regulation in eukaryotes Chromatin remodeling More kinds of control elements Promoters, Enhancers, and Silencers Combinatorial control Cell-specific
Control of Gene Expression
Control of Gene Expression 1 How Gene Regulation Works 2 Control of Gene Expression Controlling gene expression is often accomplished by controlling transcription initiation Regulatory proteins bind to
EpiQuik HAT Activity/Inhibition Assay Kit
EpiQuik HAT Activity/Inhibition Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HAT Activity/Inhibition Assay Kit is very suitable for measuring HAT activity/inhibition
Regulation of Gene Expression
Chapter 18 Regulation of Gene Expression Edited by Shawn Lester PowerPoint Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley
EpiSeeker Histone Extraction Kit
ab113476 EpiSeeker Histone Extraction Kit Instructions for Use For the extraction of histone proteins from mammalian cells and tissue This product is for research use only and is not intended for diagnostic
Genes - DNA - Chromosome. Chutima Talabnin Ph.D. School of Biochemistry,Institute of Science, Suranaree University of Technology
Genes - DNA - Chromosome Chutima Talabnin Ph.D. School of Biochemistry,Institute of Science, Suranaree University of Technology DNA Cellular DNA contains genes and intragenic regions both of which may
Chapter 9-II - Transcriptional Control of Gene Expression
Chapter 9-II - Transcriptional Control of Gene Expression Transcriptional Control of Gene Expression 9.3 RNA Polymerase II Promoters and General Transcription Factors Three types of promoter sequences
Bioinformatics of Transcriptional Regulation
Bioinformatics of Transcriptional Regulation Carl Herrmann IPMB & DKFZ c.herrmann@dkfz.de Wechselwirkung von Maßnahmen und Auswirkungen Einflussmöglichkeiten in einem Dialog From genes to active compounds
CHEM 4420 Exam I Spring 2013 Page 1 of 6
CHEM 4420 Exam I Spring 2013 Page 1 of 6 Name Use complete sentences when requested. There are 100 possible points on this exam. The multiple choice questions are worth 2 points each. All other questions
What is Epigenetics? Watch the video
EPIGENETICS What is Epigenetics? The study of environmental factors on gene expression in DNA. The molecule is called methylation controls when genes are turned on. Methylation turns off genes. Acetylation
CHAPTERS , 17: Eukaryotic Genetics
CHAPTERS 14.1 14.6, 17: Eukaryotic Genetics 1. Review the levels of DNA packing within the eukaryote nucleus. Label each level. (A similar diagram is on pg 188 of your textbook.) 2. How do the coding regions
CAYMAN CURRENTS. Epigenetics ISSUE20 IN THIS CURRENT: Epigenetic Readers Spotlight on Protein Methylation Readers DNA Methylation Readers
ISSUE20 CAYMA CURRETS Epigenetics I THIS CURRET: n Epigenetic Readers Spotlight on Protein Methylation Readers DA Methylation Readers n Inhibitors of Histone Acetylation Readers n Histone Acetylation Readers:
NUCLEUS. Fig. 2. Various stages in the condensation of chromatin
NUCLEUS Animal cells contain DNA in nucleus (contains ~ 98% of cell DNA) and mitochondrion. Both compartments are surrounded by an envelope (double membrane). Nuclear DNA represents some linear molecules
Chapter 18: Regulation of Gene Expression
Chapter 18: Regulation of Gene Expression Regulation of Metabolism Shuts off transcription Types of Feedback Negative feedback = body s response is to reduce the stimulus Ex: regulation of body temp, blood
BIOLOGY. Chapter 16 GenesExpression
BIOLOGY Chapter 16 GenesExpression CAMPBELL BIOLOGY TENTH EDITION Reece Urry Cain Wasserman Minorsky Jackson 18 Gene Expression 2014 Pearson Education, Inc. Figure 16.1 Differential Gene Expression results
Division Ave. High School AP Biology
Control of Eukaryotic Genes 2007-2008 The BIG Questions n How are genes turned on & off in eukaryotes? n How do cells with the same genes differentiate to perform completely different, specialized functions?
A Modified Digestion-Circularization PCR (DC-PCR) Approach to Detect Hypermutation- Associated DNA Double-Strand Breaks
A Modified Digestion-Circularization PCR (DC-PCR) Approach to Detect Hypermutation- Associated DNA Double-Strand Breaks SARAH K. DICKERSON AND F. NINA PAPAVASILIOU Laboratory of Lymphocyte Biology, The
36. The double bonds in naturally-occuring fatty acids are usually isomers. A. cis B. trans C. both cis and trans D. D- E. L-
36. The double bonds in naturally-occuring fatty acids are usually isomers. A. cis B. trans C. both cis and trans D. D- E. L- 37. The essential fatty acids are A. palmitic acid B. linoleic acid C. linolenic
Chapter 16: Gene Expression from Biology by OpenStax College is licensed under a Creative Commons Attribution 3.0 Unported license.
Chapter 16: Gene Expression from Biology by OpenStax College is licensed under a Creative Commons Attribution 3.0 Unported license. 2013, Rice University. CHAPTER 16 GENE EXPRESSION 429 16 GENE EXPRESSION
Unit 6: Molecular Genetics & DNA Technology Guided Reading Questions (100 pts total)
Name: AP Biology Biology, Campbell and Reece, 7th Edition Adapted from chapter reading guides originally created by Lynn Miriello Chapter 16 The Molecular Basis of Inheritance Unit 6: Molecular Genetics
Y1 Biology 131 Syllabus - Academic Year
Y1 Biology 131 Syllabus - Academic Year 2016-2017 Monday 28/11/2016 DNA Packaging Week 11 Tuesday 29/11/2016 Regulation of gene expression Wednesday 22/9/2014 Cell cycle Sunday 4/12/2016 Tutorial Monday
Bundle 5 Test Review
Bundle 5 Test Review DNA vs. RNA DNA Replication Gene Mutations- Protein Synthesis 1. Label the different components and complete the complimentary base pairing. What is this molecule called? _Nucleic
PARP-1 (cleaved) Human In-Cell ELISA Kit (IR)
ab110215 PARP-1 (cleaved) Human In-Cell ELISA Kit (IR) Instructions for Use For the quantitative measurement of Human PARP-1 (cleaved) concentrations in cultured adherent and suspension cells. This product
GENE REGULATION slide shows by Kim Foglia modified Slides with blue edges are Kim s
GENE REGULATION slide shows by Kim Foglia modified Slides with blue edges are Kim s 2007-2008 Bacterial metabolism Bacteria need to respond quickly to changes in their environment STOP GO if they have
CUSTOM SERVICES. Histone Analysis ATAC-Seq ChIP-Seq DNA Methylation Gene Regulation. for epigenetics and gene regulation research
Enabling Epigenetics Research CUSTOM SERVICES for epigenetics and gene regulation research Histone Analysis ATAC-Seq ChIP-Seq DNA Methylation Gene Regulation CUSTOM SERVICES for epigenetics and gene regulation
Metabolic collateral vulnerabilities of MTAP-deleted cancers as therapeutic opportunities Keystone on Tumor Metabolism 2017
Metabolic collateral vulnerabilities of MTAP-deleted cancers as therapeutic opportunities Keystone on Tumor Metabolism 2017 5 9 March 2017 Whistler, Canada The Challenge: Identifying Precision Medicine
Methyltransferase Fluorometric Assay Kit
Methyltransferase Fluorometric Assay Kit Item o. 700150 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE F CTETS GEERAL IFRMATI
List of Papers. This thesis is based on the following papers, which are referred to in the text by their Roman numerals.
To my family List of Papers This thesis is based on the following papers, which are referred to in the text by their Roman numerals. I Lönn, P., Dahl, M., Van der Heide, LP., Hellman, U., Heldin, C-H
LI-COR, Odyssey, Aerius, IRDye and In-Cell Western are registered trademarks or trademarks of LI-COR Biosciences Inc.
PROTOCOL PARP-1 (cleaved) In-Cell ELISA Kit (IR) 185 Millrace Drive, Suite 3A Eugene, Oregon 9743 MSA43 Rev. DESCRIPTION An In-Cell ELISA kit for measuring cleaved PARP-1 (89kDa fragment) in human adherent
DNA - DEOXYRIBONUCLEIC ACID
DNA - DEOXYRIBONUCLEIC ACID blueprint of life (has the instructions for making an organism) established by James Watson and Francis Crick codes for your genes shape of a double helix made of repeating
Systematic Analysis of single cells by PCR
Systematic Analysis of single cells by PCR Wolfgang Mann 27th March 2007, Weihenstephan Overview AmpliGrid Technology Examples DNA Forensics Single Cell Sequencing Polar Body Diagnostics Single Cell RT
DISCOVERY OF SMALL MOLECULE AND PEPTIDE-BASED LIGANDS FOR THE METHYL-LYSINE BINDING PROTEINS 53BP1 AND PHF1/PHF19. Michael Thomas Perfetti
DISCOVERY OF SMALL MOLECULE AND PEPTIDE-BASED LIGANDS FOR THE METHYL-LYSINE BINDING PROTEINS 53BP1 AND PHF1/PHF19 Michael Thomas Perfetti A dissertation submitted to the faculty of the University of North
Chapter 8: DNA and RNA
Chapter 8: DNA and RNA Lecture Outline Enger, E. D., Ross, F. C., & Bailey, D. B. (2012). Concepts in biology (14th ed.). New York: McGraw- Hill. 1 8-1 DNA and the Importance of Proteins Proteins play
Control of Eukaryotic Genes. AP Biology
Control of Eukaryotic Genes The BIG Questions How are genes turned on & off in eukaryotes? How do cells with the same genes differentiate to perform completely different, specialized functions? Evolution
UBIQUITIN & UBL SIGNALING
UBIQUITIN & UBL SIGNALING Ubiquitin, Ubiquitin-like Proteins & Their Derivatives Ubiquitin SUMO NEDD8 ISG15 FAT10 Ubiquitin & Ubl Mutants Ubiquitin & Ubl Terminal and Side Chain Derivatives Ubiquitin &
Regulation of Gene Expression
CAMPBELL BIOLOGY IN FOCUS URRY CAIN WASSERMAN MINORSKY REECE 15 Regulation of Gene Expression Lecture Presentations by Kathleen Fitzpatrick and Nicole Tunbridge, Simon Fraser University SECOND EDITION
Einführung in die Genetik
Einführung in die Genetik Prof. Dr. Kay Schneitz (EBio Pflanzen) http://plantdev.bio.wzw.tum.de schneitz@wzw.tum.de Prof. Dr. Claus Schwechheimer (PlaSysBiol) http://wzw.tum.de/sysbiol claus.schwechheimer@wzw.tum.de
Gene Expression and Heritable Phenotype. CBS520 Eric Nabity
Gene Expression and Heritable Phenotype CBS520 Eric Nabity DNA is Just the Beginning DNA was determined to be the genetic material, and the structure was identified as a (double stranded) double helix.
DNA makes RNA makes Proteins. The Central Dogma
DNA makes RNA makes Proteins The Central Dogma TRANSCRIPTION DNA RNA transcript RNA polymerase RNA PROCESSING Exon RNA transcript (pre-mrna) Intron Aminoacyl-tRNA synthetase NUCLEUS CYTOPLASM FORMATION
Figure 1: TDP-43 is subject to lysine acetylation within the RNA-binding domain a) QBI-293 cells were transfected with TDP-43 in the presence or
Figure 1: TDP-43 is subject to lysine acetylation within the RNA-binding domain a) QBI-293 cells were transfected with TDP-43 in the presence or absence of the acetyltransferase CBP and acetylated TDP-43
Supporting Information
Supporting Information Su et al. 10.1073/pnas.1211604110 SI Materials and Methods Cell Culture and Plasmids. Tera-1 and Tera-2 cells (ATCC: HTB- 105/106) were maintained in McCoy s 5A medium with 15% FBS
ab Histone Extraction Kit
ab113476 Histone Extraction Kit Instructions for Use For the extraction of histone proteins from mammalian cells and tissue This product is for research use only and is not intended for diagnostic use.
Functional characterisation
Me Me Ac Ac Functional characterisation How can we know if measured changes in DNA methylation and function (phenotype) and linked, and in what way? DNMT Dianne Ford Professor of Molecular Nutritional
Chapter 13. From DNA to Protein
Chapter 13 From DNA to Protein Proteins All proteins consist of polypeptide chains A linear sequence of amino acids Each chain corresponds to the nucleotide base sequenceof a gene The Path From Genes to
MATERIAL DATA SHEET. NOTE: Kit contains reagents sufficient for 10 x 30 μl reactions and 5 Western Blots (minigel. Reagents Provided in Kit
Lot # XXXXX ITCH/AIP4 Ubiquitin Ligase Kit Cat. # K-270 MATERIAL DATA SHEET The mammalian Itchy homolog, or ITCH, (also known as Atrophin-1-interacting protein 4 or AIP4) is a HECT domain class ubiquitin
Dissertation zur Erlangung des mathematisch-naturwissenschaftlichen Doktorgrades Doctor rerum naturalium der Georg-August-Universität Göttingen
Functional Characterization of the Histone Methyltransferase and Methyl DNA Binding Protein MDU and its Role in Epigenetic Regulation of Rbf Gene in Drosophila melanogaster Dissertation zur Erlangung des
Bio11 Announcements. Ch 21: DNA Biology and Technology. DNA Functions. DNA and RNA Structure. How do DNA and RNA differ? What are genes?
Bio11 Announcements TODAY Genetics (review) and quiz (CP #4) Structure and function of DNA Extra credit due today Next week in lab: Case study presentations Following week: Lab Quiz 2 Ch 21: DNA Biology
DNA Function: Information Transmission
DNA Function: Information Transmission DNA is called the code of life. What does it code for? *the information ( code ) to make proteins! Why are proteins so important? Nearly every function of a living