Higher Technical School of Agricultural Engineering UPCT PLANT BIOTECHNOLOGY

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1 Higher Technical School of Agricultural Engineering UPCT PLANT BIOTECHNOLOGY Degree/s: Degree in Agro-Food Engineering and Biological Systems Course: 2015/2016

2 1. Subject information Name Plant Biotechnology Subject area Specific optional subjects Module Optional subjects Code Degree programme Degree in Agro-Food and Biological Systems Engineering Study plan 2013 Centre Higher Technical School of Agricultural Engineering (ETSIA) Type Optional Teaching period Four-month period Language English and Spanish Four-month period 2 nd Course 4º ECTS 3 Hours / ECTS 30 Total workload (hours) 90

3 2. Teacher information Lecturer in charge Antonio A. Calderón García Department Agricultural Science and Technology Knowledge area Plant Physiology Office location nd Floor, ETSIA Telephone Fax URL / WEB Office hours Updated in Aula virtual at the start of the academic year Location Office 2.17, 2 nd floor ETSIA Teaching and research profile Teaching experience Research lines Degree in Chemical Sciences (Universidad Murcia) PhD in Biological Science (Universidad Murcia) Titular Professor at university (Plant Physiology) At the UPCT from 1999 Teaching quinquennia: 4 Heavy-metal hyper-accumulation and tolerance in plants, molecular and physiological biomarkers of stress in plants, conservation of threatened species by biotechnological tools, production of bioactive compounds by cell cultures Work experience Six-year research periods: 3 Other Teaching innovation Lecturer in charge Mª Ángeles Ferrer Ayala Department Agricultural Science and Technology Knowledge area Plant Physiology Office location Office 2.19, 2nd Floor, ETSIA Telephone Fax Mangeles.ferrer@upct.es URL / WEB Office hours Updated in Aula virtual at the start of the academic year

4 Location Office 2.19, 2 nd floor ETSIA Teaching and research profile Teaching experience Research lines Degree in Biological Sciences (Universidad Murcia) PhD in Biological Science (Universidad Murcia) Marie Curie Post-doctoral Fellow ENSAT (Toulouse) Titular Professor at university (Plant Physiology) At the UPCT since 2001 Teaching quinquennia: 4 Teaching Excellent Award in 2008/2009 from UPCT Heavy-metal hyper-accumulation and tolerance in plants, molecular and physiological biomarkers of stress in plants, conservation of threatened species by biotechnological tools, production of bioactive compounds by cell cultures Work experience < 1 year Six-year research periods: 3 Other Teaching innovation Lecturer in charge Catalina Egea Gilabert Department Agricultural science and technology Knowledge area Plant Physiology Office location Office 2.18, 2 nd floor ETSIA Telephone Fax catalina.egea@upct.es URL / WEB Office hours Previous appointment by Location Office 2.18, 2 nd floor ETSIA and/or by Teaching and research profile Teaching experience Degree in Chemistry from the University of Murcia PhD Biochemistry from the University of Murcia Post-doctoral Fellow University of Nottingham (United Kingdom) Associate Professor Assistant Professor: January 2000-Feb Associate Professor: February 2002-Present 14 years teaching the subject 4 teaching quinquenniums (last 2009)

5 Research lines Work experience Other Plant-pathogen interaction. Mechanisms of biological control of diseases. Induction of stresses for resistant plants with high nutritional quality. Working on issues related to plant pathogen interaction since Three granted Six-year periods:

6 3. Contents 3.1 Contents according to the study plan of the subject Unit I: Introduction to Plant Biotechnology Unit II: Plant tissue culture Unit III: Transgenic plants 3.2 Theory program (teaching modules and units) Unit I: Introduction to Plant Biotechnology (C1) Chapter 1. Introduction (C2) Chapter 2. The Plant Biotechnology laboratory Unit II: Plant tissue culture (C3) Chapter 3. Basic methodology. (C4) Chapter 4. Cell cultures. (C5) Chapter 5. Meristem culture. (C6) Chapter 6. Micropropagation (C7) Chapter 7. Somatic embryogenesis (C8) Chapter 8. In vitro production of haploid plants. (C9) Chapter 9. Techniques for plant germplasm storage and cryopreservation (C10) Chapter 10. In vitro production of secondary metabolites Unit III: Transgenic plants (C11) Chapter 11. Transgenic plants (I): Techniques for plant transformation. (C12) Chapter 12. Transgenic plants (II): Transgenics in crop improvement. (C13) Chapter 13. Transgenic plants (III): Biosafety concerns and regulatory framework. 3.3 Practice program (name and description of every practical) P1. Plant cell culture initiation and characterization. P2. Isolation of the shoot apical meristem. P3. In vitro propagation and acclimation of plants. P4. Analysis of antioxidant compounds produced by plant cell cultures. P5. Protoplast isolation and fusion. P6. Agrobacterium-mediated transformation of leaf disks.

7 4. Bibliography and resources 4.1 Basic bibliography For all units: Agricultural Biotechnology (1998). Altman, A. (ed). Marcel Dekker, Inc. Nueva York. Introduction to Plant Biotechnology, 3ª edición (2009). Chawla, H.S. CRC Press Introducción a la biotecnología vegetal métodos y aplicaciones (2001). Caballero, J.L. CajaSur For Unit 2: Functions and Biotechnology of Plant Secondary Metabolites 2 nd ed (2010). Wink, M. Wiley-Blackwell Micropropagation.-Technology and Application (1993). Debergh, P.C. y Zimmerman, R.H. (eds). Kluwer Academic Publishers. Dordrecht. Plant Cell Culture Protocols, 2ª edición (2006). Loyola-Vargas, V.M. y Vázquez-Flota, F. (eds). Humana Press. Totowa For Unit 3: Plant Tissue Culture Engineering (2006). Gupta, S.D. y Ibaraki, Y (eds). Springer. Dordrecht Complementary bibliography Functions of Plant Secondary Metabolites and their Exploitation in Biotechnology (1999). Wink, M. (ed). Sheffield Academic Press. Sheffield. 4.3 On-line resources and others Aula virtual

BIOTECHNOLOGY FOR AGRICULTURAL CROP PRODUCTION

Syllabus BIOTECHNOLOGY FOR AGRICULTURAL CROP PRODUCTION - 73902 Last update 07-11-2016 HU Credits: 2 Degree/Cycle: 2nd degree (Master) Responsible Department: field and vegetable crops-international prog.