Development of a value chain from microalgae to aquafeed

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1 Development of a value chain from microalgae to aquafeed NO DK Workshop, Kalundborg Nov 2014 Hans Kleivdal Research Head, Uni Research Adj Prof, Univ of Bergen

2 Uni Research Centre for Applied Biotechnology Research institute owned by the University of Bergen About 500 FTEs over 8 departments UR is the research tool to connect basic research at UoB with industrial applications Centre for Applied Biotechnology focus on translating marine research into projects for value creation: - Industrial Enzyme Discovery - Vaccine development - Bergen Marine Biobank - Microalgae applications

3 Focus Bioprospecting Algal metabolism - productivity PBR up-scale train cost reduction Microalgae test center for production Integrated with CO2 and heat sidestreams Integrated with processing and feed development partners Application areas Aquafeed High-value chemicals

4 Microalgae for aquafeed CO2Bio ProAlgae IBNN SIG AQUAFEED Tech Centre RCN Infrastructure Roadmap Optim.prosj. Struct Lip

5 Optimizing the value chain for aquafeed

6 Bergen Marine Biobank Biocollection of extremeophiles and microalgae Secure biodiversity Starting point for marine biodiscovery and biotechnology pipeline in Bergen

7 Multi-product Integrated biorefinery of Algae: from Carbon dioxide and Light Energy to high-value Specialties: MIRACLES (EU FP7, , 12 mill )

8 Technology Biology

9 The "ecosystem" of WP2 in MIRACLES Extreme climate conditions push towards evolutionary adaptation High biodiversity with special properties/components WP4 Industry input -what to look for -increase the productivity T2.1 Explore the natural biodiversity by proper screening regime 1. Where/when to sample 2. How to sample 3. How to screen T2.3 Map metabolism to optimize productivity -metabolic flux analysis -transcriptome analysis T2.2 Outdoor production system operated under different climates -comparative studies with identical systems to evaluate production potential -evaluate adapted best-case strains Characterize novel strains -biomass productivity -target productivity Optimize cultivation protocols - Direct the metabolism to increase productivity WP3 Downstream -characterize -valorize WP4 Application -test functionality WP5 Demonstrate -upscale & increase productivity

10 Processing for use in aquafeed? Drop-in solution: pure oil is simple to use The whole algae is valuable; protects PUFAs from oxidation, and minimize processing costs Practical aspects wrt different formats Can the extruding process be modified to accommodate new biomass properties? Nutritional value Quality

11 Techno-economic analysis Sensitivity of various factors on USD/kg EPA&DHA eq Increase EPA/DHA to 12% of DW Free CO2 & medium Reduced mixing

12 Cost development of EPA&DHA Cost of Fish oil Source:TASA og RUBIN report 210 (Wahren & Mehlin, nov 2011) Refined oil 30% Concentrated oil, 40-70% Concentrated oil, 70-90% Concentrated oil, > 90% Cost Cost (USD per kg (USD per kg) EPA&DHA equivalents) $ Algae Photosyntetic Heterotrophic Algae biomass DW Est prod. Cost (USD per kg) EPA+DHA equivalents DHA equivalents Current production cost Estimated prod cost after optimization 3-4 years , Current production cost Estimated prod cost after optimization 3-4 years - 11,5 11,5 $ With a coordinated approach and focused effort, microalgae EPA and DHA fatty acids can be price competitive with fish oil.

13 Developing a dedicated aquafeed value chain Building a coherent R&D pipeline for microalgae based aquafeed Uni Research SINTEF F&A Nofima University of Tromsø SINTEF M&C National Pilot facility NTNU - Strengthen competence and build Univ of Life Sciences capacity UiS CORE -Integrate basic research upstream NIVA -Prod designed biomass at relevant University of Bergen volumes for downstream evaluation MicroA Polarfeed Skretting EWOS Biomar NTNU

14 Innovation network on industrial algaeproduction for aquafeed Aim: - Establish national pilot production plant - Drive focussed and integrated R&D - Strengthen competence and build capacity for up-scaled production Owners:

15 CO 2 Technology Centre Mongstad (TCM) Worlds largest pilot facility for CO 2 capture Test upscaled capture technologies Capacity at tons/y CO2Bio projected pilot facility at the TCM site

16 National Microalgae Production Pilot Being constructed at Mongstad outside Bergen Finalized 2015 Q3 - scale up to 5000 L Main focus to optimize microalgae biomass and upscale for aquafeed development

17 Summary and Invitation slide, Kalundborg 26th Nov Our core focus: Value chain microalgae for aquafeed Screening high PUFA strains Piloting/upscale aquafeed development in extruded feed 2. What we offer: Bergen Marine Biobank microalgae collection under development Screening facilities PUFAs and bioactive lipids Pilot cultivation facility ( ) Broad network of collaboration partners 3. What we hope to gain from this workshop: Collaboration partners along the whole value chain Screening pipeline Strain development Cultivation Bioprocess development Exchange of ideas for proposals (Research councils, H2020, ERA net MBT)

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The National Algaepilot Mongstad:

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