Curriculum Vitae of PATRIZIA PESSINA
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1 Curriculum Vitae of PATRIZIA PESSINA PERSONAL DETAILS Birth date: 9/12/1980 Place: Milan, Italy Phone number: EDUCATION November 2009: PhD in Translational and Molecular Medicine (DIMET), University of Milano Bicocca, Milan, Italy. PhD dissertation: Necdin enhances muscle reconstitution of dystrophic muscle muscle by mesoangioblasts stem cells April 2005 (A.A. 2003/2004): degree in Biological Sciences, University of Milano Bicocca, Milan, Italy. Final grade: 101/110. Bachelor dissertation: Citoskeleton and neural stem cells: functional analysis of neurogenesis and cell migration in murine models June 1999: Secondary school certificate at Scientific Institute P.Frisi in Monza, Italy. RESEARCH AND ACADEMIC EXPERIENCES From July 2010: Post-doctoral fellow in Prof. Pura Muñoz-Cánoves laboratory of University of Pompeu Fabra at PRBB (Park of Biomedical Research of Barcelona). Description: study of molecular mechanisms involved in muscular phenotypes associated to human diseases, in the
2 context of EndoStem (Endogenous Stem Cell Therapy for Degenerative Muscle Disease) project. A.A. 07/ 08, 08/ 09 and 09/ 10 Tutor in Biology and Genetics for Medicine and Surgery Degree Course, at the Department of Medicine and Surgery of University of Milano-Bicocca, Italy. November 2009-June 2010 Research fellow in Dr Silvia Brunelli s laboratory at the Department of Regenerative Medicine-HSR, Milan, Italy. Description: Study of the role of protein Necdin in mesoangioblasts-dependent muscle regeneration process. January October 2009: PhD student in Dr. Silvia Brunelli s laboratory at the Department of Medicine, University of Milano Bicocca in collaboration with Prof. Giulio Cossu s laboratory, at the Department of Regenerative Medicine-HSR, Milan, Italy. Description: during my PhD program my project focused on the mechanisms regulating muscle regeneration by mesoangioblasts stem cells, with the final goal of improving stem cell therapy for muscular dystrophy. September December 2006: research fellow at CONSTEM (Consorzio Nazionale per la Ricerca sulle Cellule Staminali), Milan, Italy. Description: during the period at CONSTEM, my research aimed to the identification and characterization of markers of neural stem cells. October 2003 April 2005: undergraduate thesis in Prof. Angelo Vescovi s laboratory at the Department of Biological Sciences and Biotechnology of University of Milano Bicocca, Italy. Description: during my bachelor training I was involved in a project concerning adult neural stem cells and the involvement of cytoskeleton structure in neurogenesis and neuroblasts migration.
3 TECHNICAL EXPERTISES Cell Biology -Culture of neural stem cells, mouse ES cells, various cell lines -Establishment of primary culture from CNS -Isolation of muscle precursor cells -Transfections -Cell adhesion test -Cell differentiation tests Histology -Tissue preparation: criopreservation in OCT -Criostate sectioning -Immunohistochemestry and Immunofluorescence on tissue sections Microscopy -Epifluorecence microscopy -Light microscopy -Confocal microscopy -Videomicroscopy -Image processing Molecular Biology: -Cloning -PCR, RT PCR, qpcr -Two-Hybrid system Biochemistry -Protein purification and Western Blotting. Animal experimentation -Mice handling -Dissection -Subcutaneous, intraperitoneal and intramuscular injections
4 AWARDS Multi-Colour Flow Cytometry Course 7-11 February 2001, University of British Columbia, Vancouver, Canada PUBLICATIONS Necdin enhances muscle reconstitution of dystrophic muscle by vessel associated progenitors, by promoting cell survival and myogenic differentiation Pessina P, Conti V, Tonlorenzi T, Touvier T, Meneveri R, Cossu G and Brunelli S Cell Death and Differentiation, In Press Aberrant repair and fibrosis development in skeletal muscle Muñoz-Cánoves P, Mann CJ, Perdiguero E, Kharraz Y, Aguilar S, Pessina P and Serrano AL Skeletal Muscle 2011, 1:21 Skeletal muscle of gastric cancer patients expresses genes involved in muscle regeneration Pessina P, Conti V, Pacelli F, Rosa F, Foglietto GB, Brunelli S, Bossola M. Oncol Rep Sep;24(3): Necdin is expressed in cachectic skeletal muscle to physiologically protect fibers from tumorinduced wasting Sciorati C, Touvier T, Buono R, Pessina P, François S, Perrotta C, Meneveri R, Clementi E, Brunelli S J Cell Sci Apr 15;122(Pt 8): SCIENTIFIC COMMUNICATIONS Epithelial Plasticity and Epithelial to Mesenchymal Transition January 2011, Vancouver, Canada Cellular origin of myofibroblasts in dystrophic muscle Pessina P, Zamora M, Jardi M, Muñoz-Cánoves P
5 ISSCR 7 th Annual Meeting 8-11 July 2009, Barcelona, Spain Role of Necdin in mesoangioblasts dependent skeletal muscle differentiation and regeneration Pessina P, Conti V., Diaz-Manera J., Touvier T., Cossu G. and Brunelli S. Telethon Convention March 2009, Riva del Garda (TN), Italy ASCB 48 th Annual Meeting December 2008, S.Francisco, CA Eurostemcell Summer School Stem Cells and Regenerative Medicine September 2007, Hydra, Greece LANGUAGES reading writing verbal skills English excellent excellent excellent Spanish excellent excellent excellent COMPUTER KNOWLEDGE Perfect command of Operating Systems (Windows XP, Vista, Apple Mac OS X); Excellent knowledge of Microsoft Office tools (Word, Excel, PowerPoint); Perfect command of 2D graphic design applications such as Adobe Photoshop and Illustrator; Excellent knowledge of software for research ApE. Knowledge of databases as NCBI and NEXTBIO. REFERENCES Available upon request Ai sensi della legge 675/ 96 autorizzo il trattamento dei miei dati personali per le finalità di ricerca e selezione del personale
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