COLOSS Work Shop. Genetic discrimination Lab of Agricultural Zoology &Entomology. Agricultural University of Athens
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1 Action FA0803 COLOSS Work Shop Genetic discrimination Lab of Agricultural Zoology &Entomology Agricultural University of Athens 75, Iera Odos Str, 11855, Athens, Greece. Tel.: Fax: AGRICULTURAL UNIVERSITY OF ATHENS
2 Table of contents Agenda... 3 Completion of genetic analysis data... 5 Meixner Marina, Kryger Per, Evgeniya Ivanova, Bouga Maria... 5 Genetic variability of honey bee origins used in the GEI experiment... 6 Evgeniya Neshova Ivanova... 6 List of participants
3 Action FA0803 COLOSS Work Shop Genetic discrimination Lab of Agricultural Zoology &Entomology Agricultural University of Athens 75, Iera Odos Str, 11855, Athens, Greece. Tel.: / Fax: Agenda TIME (Thursday) 10:00-12:00 Welcome and organizational matter 12:00 13:30 Presentation on the results from microsatellites analysis on the samples used in the GEI experiment. 13:30 15:00 Lunch 15:00 17:00 Discussion on the results from microsatellites analysis on the samples used in the GEI experiment. 17:00 19:30 Visit the centre of Athens 20:00 Welcome dinner (Friday) 09:00 10:30 Presentation on the results from isoenzymic analysis on the samples used in the GEI experiment. 10:30 11:00 Coffee break 11:00 13:30 Discussion on the results from isoenzymic analysis on the samples used in the GEI experiment. 13:30 15:00 Lunch 15:00 19:00 Presentation on the results from geometric morphometry analysis on the samples used in the GEI experiment. 20:00 open Social dinner 3
4 (Saturday) 09:30 11:00 Discussion on the results from geometric morphometry analysis on the samples used in the GEI experiment 11:00 11:30 Coffee break 11:30 13:30 Discussion on the progress of mitochondrial DNA analysis and classical morphometry analysis on the samples used in the GEI experiment. 13:30 15:00 Lunch 15:00 16:00 Conclusions 16:00 End of the workshop Local Organizer: Dr. Maria Bouga 4
5 Completion of genetic analysis data Meixner Marina 1*, Kryger Per 2, Evgeniya Ivanova 3, Bouga Maria 4 1* LLH Bieneninstitut Kirchhain, Erlenstr. 9, Kirchhain, Germany Tel: +49 (0) / Fax: +49 (0) marina.meixner@llh.hessen.de 2 University of Aarhus, PBS-DJF, Research Centre Flakkebjerg, Denmark 3 Department of Developmental Biology, University of Plovdiv, Bulgaria 4 Lab of Agricultural Zoology & Entomology, Agricultural University of Athens, Greece One of the main goals of COLOSS WG 4 is to establish a common protocol for the discrimination of honey bee populations. In Europe, different methods are used to determine the subspecies origin of honey bees. In WG4, different methods have been applied to analyze samples of the colonies that are part of the common GEI experiment. Data are obtained from different approaches: geometric and classical morphometry analyses, microsatellite, mtdna and isoenzymic analyses. The data obtained from each different method have been statistically proceeded in order to discriminate the samples of the common GEI experiment. Each method will be accordingly estimated and a protocol with the potential of common use will be provided. 5
6 Genetic variability of honey bee origins used in the GEI experiment Evgeniya Neshova Ivanova University of Plovdiv Paisii Hilendarski, Faculty of Biology, Department of Developmental Biology, Section of Genetics 24, Tzar Assen Str. Plovdiv 4000 Bulgaria Different methods are used to determine the subspecies origin of honey bees. COLOSS WG4 Diversity and vitality has the purpose to find common approaches for discrimination of honey bee populations in Europe. In this aspect, in common GEI (genotype environment interactions) experiment of COLOSS WG4, data of enzyme analysis will be combined with microsatellite and mitochondrial DNA data, and classical and geometrical morphometrics in order various methods to be compared. In this investigation, genetic variation in 15 honey bee populations of Apis mellifera mellifera, A. m. carnica, A. m. ligustica, A. m. macedonica and A. m. siciliana located in different regions in Europe was studied on different enzymic systems. Allozyme analysis revealed that studied loci were polymorphic in all populations studied. Three to six alleles were detected at the loci investigated. The allele frequencies, the mean number of alleles per locus, the observed and expected heterozygosities, mean F ST value and genetic distance were calculated. Data from this study will contribute to the documentation of the subspecies genetic origin involved in the common experiment and to the establishment of a published and accessible reference data-base that will be valuable for scientists and apiculturists working in the field of European honey bee biodiversity and conservation. 6
7 List of participants Name Country Bouga Maria Greece Ivanova Evgeniya Bulgaria Kryger Per Denmark Meixner Marina Germany 7
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