Lab on a chip fotònics i electroquímics

Transcription

1 Lab on a chip fotònics i electroquímics Grup de Transductors Químics gtq.imb-cnm.csic.es Xavier Muñoz xavier.munoz@imb-cnm.csic.es 21 Maig, 2015

2 Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC) Main Clean Room 1500 m 2 Class Basement Clean Room 40 m 2 Class Largest CR in South europe 21 Maig, 2015

3 Grup de Transductors Químics Lab on a Chip A lab-on-a-chip (LoC) is a system that merges all the capabilities of a lab in a single millimiter-size system Key Advantages Small sample volume (μl) Enhanced response time Portable equipment Low consumption Maig, 2015

4 Towards a Lab on a chip Nanoelectronics Nanomedicine Nanofluidics Nanophotonics

5 Elements included in a Lab on a chip Passive elements: Channels, reactors, mixers Active elements Pumps, valves Sensors Photonic, electrochemical. at the micron-size level!! Detection Inlets microfluidics 21 Maig, 2015

6 What do we apply our Lab on chips for? FOOD ph, ions Ascorbic acid, lactate Pathogens (i.e. Bacteria) Wine: Ethanol, sugar, acidity, color, grape variety, vintage... ENVIRONMENT ph, conductivity, Ions Organic matter chemical oxygen demand Chlorine, turbidity Heavy metals Pathogens (i.e. Bacteria) Matrices: Wine Milk Yoghurt Beverages Soup. Scenarios: Lakes Purification plants Tap water Wastewater treatment plants 21 Maig, 2015

7 What do we apply our Lab on chips for? BIOMEDICAL ANALYSIS Metabolites in biological liquids: ph, Na, Ca, glucose, urea, Illness indicators: Cancer, Reumatoid arthritis Drugs: Dopamine, paracetamol Cell culture: Celular methabolism, toxicity, cell counting Saccharomyces cerevisiae Vascular smooth muscle cells (VSMC) Escherichia coli 21 Maig, 2015

8 Multiparametric system: Electronic Tongue Analyze food products such as wines, beverages and soups Classification approach Quantification approach 21 Maig, 2015

9 Electrical readout system Impedimetric analysis of bioarrays developed on glass slides Analysis of boldenone hormone Array 36 gold electrodes 2 mm Applied to the measurement of proteins and haptens different immunoassay formats. Analytical performance similar to fluorescent scanner approaches 21 Maig, 2015

10 Wax-based lab on a chip platform Patterning of wax is used to fabricate microfluidic structures (reservoirs, pumps, valves) Wax patterning down to 25 µm LOC with amperometric detection Operation of a wax valve 21 Maig, 2015

11 Hybrid Photonics Polymer-based micro-optics/photonics elements Microlenses Micromirrors Flat air mirror Focusing air mirror Air n=1.00 PDMS n=1.41 Parallel beams Beam concentrator 21 Maig, 2015

12 Transmittance (db) Hybrid Photonics Polymer-based micro-optics/photonics elements Polymeric absorbance microfilters Xerogel solid-state light emitters (SSLE) Non-doped Alexa Rhodamine B -5 Methyl orange -10 Quinoline yellow Phenol red Crystal violet Wavelength (nm) CdSe QDs 21 Maig, 2015

13 Photonic lab on a chip with integrated light emitters H 2 O 2 detection via HRP Input fluidic port air mirror Self-alingment channel microlense Prepolymer solution intlet emitter air mirror Interrogation region Ouput fluidic port Prepolymer solution outlet LOD: 2.73 ± 0.05 μm Sensitivity: 2.9 ± 0.2 A.U./mM. PhLoC including: Xerogel emitters, microlenses, micromirrors, channels for fiber optics alignment and functionalized enzymatic reactor 21 Maig,

14 Biophotonic Lab on a chip Simultaneous supernatant and cell culture screening Cell seeding inlet Self-alignment channel Microlens Fluidic inlet Size-exclusion microfilters Retention reactor Monitoring reactor Fluidic outlet Air mirrors 21 Maig,

15 Biophotonic Lab on a chip Integrated size-exclusion microfilters 21 Maig, /05/

16 Biophotonic Lab on a chip Integrated size-exclusion microfilters 21 Maig, /05/

17 Absorbance (A.U.) Biophotonic Lab-on-chip Size-dependent microorganism separation Retention reactor Monitoring reactor Trapping Rinsing Time (min) Suitable for cell culture purification, microorganism separation while performing real time screening Maig,

18 Acknowledgements The research leading to these results has received funding from the European Research Council under the European Community's Seventh Framework Programme (FP7/ ) / ERC grant agreement n Project funded by the European Commission within the 7th Framework Programme (FP7/ ) Grant Agreement nº Maig,

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