Certificate of qualification as a Professional Biologist University of L Aquila.
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1 CURRICULUM VITAE CRISTIANA BORRELLI EDUCATION AND TRAINING January March 2017 AMC Academic Medical Centre, Amsterdam (Unipharma graduates Project) Project: Study of senescent phenotype in murine model of renal ischemia reperfusion injury in WT and mice lacking TREM1. Skills: Mouse handling, sacrifice, organ harvesting and processing for analysis. Competences: Murine models of renal injury such as renal ischemia/reperfusion, unilateral uretheral obstruction and pyelonephritis. February 2015 Certificate of qualification as a Professional Biologist University of L Aquila. November 2014 October 2017 PhD student in Immunology and Immunopathology PhD School in Immunological, Hematological and Rheumatological Sciences Laboratory of Molecular Immunology and Immunopathology Prof. Angela Santoni, Department of Molecular Medicine Faculty of Pharmacy and Medicine Sapienza University of Rome Viale Regina Elena, Rome, Italy Topic of the thesis: Molecular mechanisms involved in the regulation of NK cell activating ligand expression in human cancer cells in response to the chemotherapeutic agent Vincristine. October 2014 Master s degree in Medical Biotechnology 110/110 cum laude Sapienza, University of Rome. Piazzale Aldo Moro 5, Rome, Italy. 110 cum laude/110 Title of the experimental thesis: Vincristine regulates the expression of NKG2D and DNAM1 activating ligands in tumor cells by modulating their susceptibility to Natural Killer cell mediated lysis : Laboratory of Prof. Angela Santoni (Laboratory of Molecular Immunology and Immunopathology), Department of Molecular Medicine. Sapienza University of Rome Viale Regina Elena, Rome, Italy Training coordinator: Dr Alessandra Soriani My Master s degree thesis focuses on the interplay between NK cells and Multiple Myeloma. In particulary I m currently analyzing the mechanisms through which Multiple Myeloma cells escape from NK cells mediated recognition and killing. Techniques used: Cell cultures. RNA estraction and Reverse transcription. High-Fidelity PCR. Molecular cloning. Protein extraction. Western Blotting. Immunoprecipitation. Flow Cytofluorimetry. Fluorescence Mycroscopy. Citotoxicity assay. European Union,
2 September 2011 Techniques and diagnostic of autopsies and Histopathological procedures 1st level Master Techniques and diagnostic of autopsies and Histopathological procedures Institute of Pathological Anatomy, Università Cattolica del Sacro Cuore Largo Agostino Gemelli, Rome, Italy. April 2011 Bachelor degree 108/ /110 Title of the experimental thesis: Monitoring of intracellular HIV-1 mutations associated at drug resistance in HIV-positive patients responder to antiretroviral therapy. Laboratory of Prof. Ombretta Turriziani (Laboratory of Medical Virology), Department of Molecular Medicine - Faculty of Pharmacy and Medicine Sapienza University of Rome. PERSONAL SKILLS Mother tongue(s) Italian Other language(s) UNDERSTANDING SPEAKING WRITING Listening Reading Spoken interaction Spoken production English: B2 C1 B2 C1 C1 FCE Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user Common European Framework of Reference for Languages Spanish: Beginner European Union, Page 2 / 5
3 Job-related skills Cell Biology and Cellular Immunology: Standard protocols of cellular culture for human and murine cell lines. Multicolor immunofluorescence assay (FACS Calibur, FACS Canto II) and FACS data analysis with FlowJo and GraphPad. Leucocytes purification from peripheral blood and human bone marrow aspirates. Isolation of myeloid and lymphoid cell population with magnetic beads. Cell proliferation assays and apoptosis assays (MTT, CFSE, Cell cycle, PI-BrdU analysis, Annexin V). NK cell degranulation (CD107a) assay. Biochemistry and Molecula Biology: Protein extraction and purification. Protein electrophoresis. Western blot. ELISA assay. RNA extraction and cdna production from tissues and cell lines. PCR, RT-PCR and Real-Time PCR (Taqman and SYBRgreen). Electrophoresis of nucleic acids. Luciferase assay. Transfection in human cell lines. Retroviral/Lentiviral gene infection in human cell lines. β-galactosidase staining. Exosomes isolation and purification. Microscopy assay. Computer skills Excellent command: Microsoft Office TM suite (Word, Excel, PowerPoint) Data Analysis (ImageJ, FlowJo, GraphPad) ADDITIONAL INFORMATIONS PUBLICATIONS p38 MAPK differentially controls NK activating ligands at transcriptional and posttranscriptional level on multiple myeloma cells. Alessandra Soriani*, Cristiana Borrelli*, Biancamaria Ricci, Rosa Molfetta, Alessandra Zingoni, Cinzia Fionda, Silvia Carnevale, Maria Pia Abruzzese, Maria Teresa Petrucci, Maria Rosaria Ricciardi, Giuseppe La Regina, Erica Di Cesare, Patria Lavia, Romano Silvestri, Rossella Paolini, Marco Cippitelli, Angela Santoni. OncoImmunology, IMPACT FACTOR: *these authors contributed equally to this work Inhibition of Bromodomain and Extra-Terminal (BET) proteins increases NKG2D ligand MICA expression and sensitivity to NK cell-mediated cytotoxicity in Multiple Myeloma cells: role of cmyc/irf4. Maria Pia Abruzzese, Maria Teresa Bilotta, Cinzia Fionda, Alessandra Zingoni, Alessandra Soriani, Elisabetta Vulpis, Cristiana Borrelli, Beatrice Zitti, Maria Teresa Petrucci, Maria Rosaria Ricciardi, Rosa Molfetta, Rossella Paolini, Angela Santoni and Marco Cippitelli. Journal of Hematology & Oncology, IMPACT FACTOR: Natural Killer cell response to Chemotherapy-Stressed Cancer Cells: Role in Tumor Immunosurveillance Alessandra Zingoni, Cinzia Fionda, Cristiana Borrelli, Marco Cippitelli, Angela Santoni, Alessandra Soriani. Frontiers in Immunology, IMPACT FACTOR: 6,4 Drug-induced senescent Multiple Myeloma cells elicit NK cell activation and proliferation by direct or exosomes-mediated IL-15 trans-presentation.. Borrelli C., Ricci B.M., Vulpis E., Zingoni A., Cippitelli M., Fionda C., Ricciardi M.R., Petrucci M.T., Santoni A, Soriani A. Under revision. TREM-1 limits the maladaptive repair following acute kidney injury. Tammaro A*., Borrelli C.*, Soriani A., Santoni A., Kors L., Florquin S. Manuscript in preparation. *these authors contributed equally to this work European Union,
4 CONFERENCES January 23, 2015 BeMM Symposium 2015 (PhD Cycle XXVII) Sapienza University of Rome, Italy Drug-treated senescent Multiple Myeloma cells promote NK cells immune surveillance (poster) Ricci B, Borrelli C, Iannitto ML, Fionda C, Cippitelli M, Ponzetta A, Bernardini G, Zingoni A, Soriani A and Santoni A. June 19-20, 2015 International Retreat of PhD Students in Immunology University Magna Graecia,Catanzaro (Italy) Analysis of molecular mechanisms regulating Natural Killer cell activating ligand induction on Vincristine-treated Multiple Myeloma cells (poster) Paolini, Marco Cippitelli, Alessandra Soriani and Angela Santoni February 18, 2016 Basic and advanced Flow Cytometry Course Humanitas Clinical and Research Centre. May 7-14, th ENII Immunology Summer School Porto Cervo, Sardegna (Italy) Oral presentation: Analysis of molecular mechanisms regulating Natural Killer cell activating ligand induction on Vincristine-treated Multiple Myeloma cells. Paolini, Marco Cippitelli, Alessandra Soriani and Angela Santoni. May 25-28, 2016 SIICA 2016, X National Congress of the Italian Society of Immunology, Clinical Immunology and Allergology Abano Terme (Italy) Analysis of molecular mechanisms regulating Natural Killer cell activating ligand induction on Vincristine-treated Multiple Myeloma cells. (poster) Paolini, Marco Cippitelli, Alessandra Soriani and Angela Santoni. May, 28-31, 2017 SIICA 2017, XI National Congress of the Italian Society of Immunology, Clinical Immunology and Allergology Abano Terme (Italy) Drug-induced senescent Multiple Myeloma cells elicit NK cell activation and proliferation by direct or exosome-mediated IL-15 trans-presentation. Cristiana Borrelli, Biancamaria Ricci, Elisabetta Vulpis, Alessandra Zingoni, Marco Cippitelli, Cinzia Fionda,Maria Rosaria Ricciardi,Maria Teresa Petrucci, Alessandra Soriani, and Angela Santoni September, 27-29, 2017 VIBes in Biosciences, International PhD symposium (Ghent, Belgium). European Union, Page 4 / 5
5 AWARDS AND HONOURS UNIPHARMA GRADUATES (ERASMUS+) PROJECT Scholarships for PhD students for internships at European research centers in the chemical, biological, biotechnological, pharmaceutical and pharmacological fields. SIICA ADVANCED SCHOOL OF IMMUNOLOGY Bari,26-28 May 2017 Award 3 Best PhD students of the advanced school of immunology. (Citation from The SIICA School of Immunology 2017 : a gathering for NGS (Next Generation Scientists), Eur. J. Immunol., 2017). Winner of a travel grant for the international PhD symposium, VIBes in Biosciences - Ghent, Blegium, September Organizer of the Biology and Molecular Medicine (BeMM) symposium for PhD students. Sapienza, University of Rome, Italy, 20 Novembre, FIRMATO CRISTIANA BORRELLI European Union,
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