European Technology Platform for Nanomedicine. Inês Mendes Pinto, PharmD, PhD Portugal
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1 European Technology Platform for Nanomedicine Inês Mendes Pinto, PharmD, PhD Portugal
2 INL International Iberian Nanotechnology Laboratory General presentation of the Institute 1) Location: Braga- Portugal 2) Status: Intergovernmental Organisation 3) Projected Researchers:~200 postdoctoral scientists 4) Projected Total Staff: ~ 400 people 5) Research Space: 40 Principal Investigators labs Braga 2
3 Nanomedicine Nanofabrication Nanomaterials Synthesis Nanocharacterization Biomarkers Nanoengineering and systems integration Modelling Cell therapies Diagnostics Imaging solutions
4 Development of a new diagnostic tool for epithelial tumors Epithelial cell contractility - Depends on the assembly and dynamics of the cellular skeleton (=cytoskeleton). - Hypercontractility of the cytoskeleton correlates with epithelial tumor proliferation and metastasis. Cytoskeleton Braga, April 24 th, 2014 ines.m.pinto@inl.int
5 Development of a new diagnostic tool for epithelial tumors Epithelial cell cytoskeleton Structure Physical-Chemistry Prediction of cell contraction rate Identification of molecular traits that affect both contraction and metastatic potential Braga, April 24 th, 2014 ines.m.pinto@inl.int
6 ENVIRONMENT MONITORING, SECURITY AND FOOD QUALITY CONTROL Project: DETECTION OF WATERBORNE TOXINS IN FOOD AND WATER FOR HUMAN CONSUMPTION Diagnostic markers for vulnerable atherosclerotic plaques Need: Coronary Angiography detects flow blockage and other atherosclerotic plaques: vulnerable or stable? Project Concept Cardiac Catheterization: Optical coherence tomography Vulnerable? Biomarkers for vulnerability validation in human coronary arteries x Vulnerable plaque Stable plaque In-house testing mockup (mimicking in vivo scenario) marina.brito@inl.int
7 Magnetoresistive Platform for Medical Diagnosis Development of surface biochemistry Production of magnetic nanoparticles Microfabrication of MR sensor Design of microelectronic board: chip control and read-out Microfluidic system design and fabrication Verónica Romão
8 Nanoparticles for cancer treatment with hyperthermia 1 - MNP + protein 2 - MNP + protein + mab Add recognition molecule (ex. mab) 4 - Magnetic field application 5 Magnetic heating 3 Specific binding Cell death membrane Cancer Treatment vania.vilasboas@visitor.inl.int
9 Cell viability (% of control) Magnetic hyperthermia promotes tumor cell death Particles only Particles only + HT PrestoBlue reduction assay Functionalized particles Functionalized particles + HT Time pos-ht (h) Fig. 1 Human glioblastoma cell viability at different time-points after hyperthermia treatment (HT). Cells were fully killed only when functionalized particles were used in association with HT application. No significant effects were observed when particles only were used, even when HT was applied to these samples. a) b) Fig. 2 - Scanning Electron Microscopy images of human glioblastoma cells. a) Untreated; b) cells with functionalized particles after HT treatment. vania.vilasboas@visitor.inl.int
10 Magnetic Resonance Imaging Cell tracking of stem cells in brain isquemia therapies (in collaboration with CHUS, Santiago de Compostela) Healthy animal (without cells-mnps) Healthy animal (1x10 6 cells-mnps) Ischemic animals (1x10 6 cells-mnps) cells) Images from partner Clinical Neurosciences Group (LINC), CHUS
11 Magnetic Imaging and Spectroscopy Magnetrodes Development a new generation of tools for local magnetic imaging and spectroscopy at the neuron scale in order to model the electromagnetic response of a neuron. 50μm thick electrodes - Detecting the magnetic field generated by the currents in the low-frequency signals <1kHz - Detecting the Nuclear Magnetic Resonance (NMR) signal for the chemical analysis of the area concerned (few 100 µm3); MHz range signals CEA-Saclay (probes), INESC-MN (probes), Aalto Univ.(modelling), ESI-Frankfurt (in vivo), CNRS-Gift sur Yvette (in vitro)
12 Scientific Building INL Campus Main Scientific Building Laboratories 7,500 m 2, Cleanroom 1000 m 2, Auditorium and other public areas 4,800 m 2, technical areas 3,500 m 2, Administrative areas 700 m2.
13 Scientific Building / Clean Room e-beam Optical litho Wet bay CNT CMP PVD II RIE PVD I PECVD Ion Mill+SIMS Anal. BIO Gowning character, Thin Film SC
14 Scientific Building / High Accuracy Labs STM/MBE spare -XRD films SAXS -AFM Lab -Env. SEM -XPS -200kV Cs TEM/STEM -DUAL FIB/HRSEM -SHIELDED ROOM (instrumentation) - TEM Sample prep Jose Rivas, Director General INL
15 Scientific Building / Biocentral Labs Bio imaging laboratory (LSM, TIRFM and widefield) Biocentral Lab Microbiology laboratory Animal cell laboratory Central biochemistry laboratory
16 International and National Collaborations INL
17 What we can offer
18 What we can offer
19 Thank you!
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