Supplemental Data. Wang et al. (2016). Plant Cell /tpc
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1 Supplemental Figure 1. Time-Course Analysis of Storage Proteins during Endosperm Development of the Wild-type 9311 and the gpa4-1 Mutant. (A) SDS-PAGE analyses of seed storage proteins during wild-type and gpa4-1 endosperm development. Blue triangles indicate the 57-kDa proglutelins. Black and red hollow arrows denote the acidic and basic subunit, respectively. DAF, days after flowering. (B) Immunoblot analysis showing the delayed accumulation of glutelins, 26-kDa α-globulins, 13-kDa and 10-kDa prolamins in the gpa4-1 endosperm. 1
2 Supplemental Figure 2. RT-qPCR Assay of the Expression of Representative Genes Coding for Storage Proteins in 12 DAF Endosperm. All of the genes analyzed were down-regulated in the gpa4-1 mutant compared with wild type. DAF, days after flowering. Values are mean ± SD. n = 3. 2
3 Supplemental Figure 3. Immunoblot Analyses Showing Defective Export of Storage Proteins from the ER in gpa4-1. (A) Immunoblot analysis of GluA1, GluB1, α-globulin, 16-kD prolamin (Pro16), 13-kD prolamin-a (Pro13a), 13-kD prolamin-b (Pro13b), 10-kD prolamin (Pro10), and VPE1 showing their distribution in various differential centrifugation fractions using their specific antibodies. P2, pellet obtained following centrifugation at 2000g; P13, pellet obtained following centrifugation at 13,000g; P100, pellet obtained following centrifugation at 100,000g; S100, supernatant obtained following centrifugation at 100,000g. Blue triangles indicate the 57-kD proglutelins. Arrows indicate different forms of VPE1. pv, VPE1 precursor; iv, intermediate VPE1; mv, mature VPE1. (B) Immunoblot analysis using specific antibodies for chaperone proteins. The accumulation of Bip1, PDIL1-1, and Hsp90 proteins is increased in the gpa4 mutants compared with the corresponding wild types. (C) Expression analysis of ER stress response-related genes in 12 DAF endosperm. The transcript levels of these genes are highly elevated in the gpa4-1 mutant. DAF: days after flowering. Values are mean ± SD. n = 3. 3
4 Supplemental Figure 4. Rescue of the gpa4-2 Mutant Phenotype by the GFP-GOT1B Fusion Construct. (A) to (D) The fusion construct GFP-GOT1B under the control of GOT1B endogenous promoter rescues the grain appearance (A), the storage protein composition pattern (B), and the storage protein trafficking defects (C and D) of the gpa4-2 mutant. GL1 to GL3 denote the grains from three independent T1 transgenic lines. Stars represent the first type of novel structure with a glutelin core and a prolamin periphery. Bars = 2 μm in (C), and 5 μm in (D). 4
5 Supplemental Figure 5. Localization Pattern of GFP-GOT1B Fusion Protein. (A) GFP-GOT1B is localized in the punctate structures in the root cells of GFP-GOT1B transgenic lines. (B) and (C) Localization of GFP-GOT1B in N. benthamiana leaf epidermal cells. (B) GFP-GOT1B shows a typical punctate pattern in cells with lower expression. (C) Faint signals in ER networks are observed in cells with higher expression. Triangles indicate the punctate structures of GFP-GOT1B. Arrows indicate the faint signals of GFP-GOT1B in ER networks. Bars = 10 µm. 5
6 Supplemental Figure 6. Both the N- and C-termini Are Essential for the Proper Localization of GOT1B. (A) Scheme of GOT1B N- and C- terminus mutation constructs for expression in the leaf epidermal cells of N. benthamiana. TMD, transmembrane domain. (B) to (G) Typical subcellular localization patterns of the various GFP-fused GOT1B mutant forms co-expressed with a known ER marker (mrfp-hdel) in N. benthamiana leaf cells. Bars = 10 μm in (B to G). GFP-GOT1B * indicates different mutant isoforms of GFP-GOT1B as shown in (A). 6
7 Supplemental Figure 7. Interaction between GOT1B and Sec23. (A) Y2H assay showing that GOT1B specifically interacts with the COPII component Sec23c. (B) GOT1B interacts with Sec23b and Sec23c in the split-ubiquitin based Y2H assay. (C) BiFC assay showing that GOT1B can interact with both Sec23b and Sec23c in leaf epidermal cells of N. benthamiana. The Golgi-localized membrane proteins COG3 and COG8 (a pair of interacting proteins, Tan et al., 2016) were used as the negative control. Bars = 10 μm. (D) No detectable Sec23c protein was immunoprecipitated by anti-sar1b antibodies in the gpa4-1 mutant. (E) RT-qPCR assay showing that Sec23c has the highest expression in 12 DAF endosperm of the wild type. (F) Y2H assay showing that Got1p and human Got1 (hgot1a and hgot1b) not only interact with their corresponding Sec23, but also cross interact with rice Sec23c. 7
8 Supplemental Figure 8. Immunoblot Analyses Showing the Specificity of Antibodies. (A) to (E) Immunoblot analyses showing the specificity of antibodies for GOT1B (A), Sar1b (B), Sar1c (C), Sec23c (D), and Sec12b (E). (A) GOT1B protein can be detected only in IP sample precipitated by anti-got1b antibodies. Total extracts were loaded for immunoblot assays in (B) to (E). T, total extract; W, wash-off solution; IP, immunoprecipitation. Arrows indicate the corresponding protein bands. 8
9 Supplemental Figure 9. Rescue of the gpa4-2 Mutant Phenotypes by Yeast GOT1. (A) and (B) Yeast Got1 rescues the grain appearance (A), and the storage protein composition pattern (B) of the gpa4-2 mutant. YL1 to YL3 denote the grains from three independent T1 transgenic lines. 9
10 Supplemental Figure 10. The Seeds of Transgenic Rice Lines Expressing AtSar1b-GFP Show No Difference from Their Corresponding Recipient Plants. (A) and (B) The grain appearance (A) and the storage protein composition pattern (B) of Kinmaze and gpa4-2 transformed with AtSar1b-GFP. 1 to 4 are transgenic lines using Kinmaze (1 and 2) or gpa4-2 (3 and 4) as the recipients, respectively. 10
11 Supplemental Table 1. Comparison of Important Agronomic Traits between Wild Type and gpa4 Mutants Characteristic 9311 gpa4-1 Kinmaze gpa4-2 Plant height (cm) ± ± 4.7 ** 95.7 ± ±1.6 Panicle length (cm) ± ± ± ± 1.20 Tiller number 6.58 ± ± ± ± ,000-grain weight (g) ± ± 0.03 ** ± ± 0.13 ** Protein content (mg/grain) 2.88 ± ± 0.02 ** 2.05 ± ± 0.13 ** Amylose content (mg/grain) 4.56 ± ± 0.05 ** 3.02 ± ± 0.10 ** The asterisks indicate statistical significance between the gpa4 mutants and the corresponding wild type, as determined by a Student s t-test (**P < 0.01). 11
12 Supplemental Table 2. Segregation of Mutant Phenotype in Reciprocal Crosses between the Wild Type and the gpa4-1 Mutant. Cross Normal 57H/Floury X 2 3:1 gpa4-1/ /gpa
13 Supplemental Table 3. The Distribution of COPII-Related Proteins in the Endomembrane System P2 P13 P100 S100 Sar1b ± ± ± ± 1.72 gpa ± 0.61** ± 1.56** ± 1.21** ± 1.84 Sar1c ± ± ± ± 0.94 gpa ± 1.52** ± 0.68** ± ± 1.32 Sec23c ± ± ± ± 2.73 gpa ± ± ± ± 3.15 Sec12b ± ± ± ± 0.23 gpa ± ± ± ± 0.25 The asterisks indicate statistical significance between the wild type and the gpa4-1 mutant, as determined by a Student s t-test (**P < 0.01). 13
14 Supplemental Table 4. Comparison of Important Agronomic Traits between AtSar1b-GFP Transgenic Rice Lines and Their Corresponding Recipient Plants. Kinmaze AtSar1b-GFP /Kinmaze gpa4-2 AtSar1b-GFP /gpa4-2 Plant height (cm) 93.8 ± ± ± ±2.3 Panicle length (cm) ± ± ± ± 1.23 Tiller number 6.87 ± ± ± ± grain weight (g) ± ± ± ±
15 Supplemental Table 5. Primers Used in This Study. Usage Primer name Sequence G1-F 5' TTCCCACCACCTCCTCCG 3' G1-R 5' GCCACTCCTCCACGCACA 3' G3-F 5' CTGCCCCCTTGCCCCTTC 3' G3-R 5' CTCACCACGCCACCCTCG 3' G4-F 5' TGCTGCTCCGCTACACAAA 3' Primers for fine mapping G4-R 5' CCTCCTTCCCCTCCTCACT 3' G9-F 5' TAGCAGCTACCTCACCGC 3' G9-R 5' ACCAGGACCTCCCGACCC 3' G11-F 5' ACCTGCGAACCACCACGGAC 3' G11-R 5' GCGAGAGGCGAAGGAAATAG 3' G15-F 5' GTGCTGCTTTAGCCGTTTAC 3' G15-R 5' TCAGGGTGTGGGAATGGTAG 3' GluA1-F 5' TGATGGTGAAGTGCCAGTTGTTGC 3' GluA1-R 5' ACGCCTGTATGCTTGAGGGTTTCT 3' GluB2-F 5' TGTCCTTCGCCCTGGACAACTATT 3' GluB2-R 5' TGGTAAGGCGCGGAATACTGAGTT 3' GluC1-F 5' ATGTAAAGTTCAACCGCGGCGATG 3' GluC1-R 5' TCTCGTTGATCTGCCACTCAGCAT 3' GluD1-F 5' AAATGAGCAGTTTCGATGCGCTGG 3' GluD1-R 5' TGTTCGAGTATCGAGGCACCACAA 3' RT-qPCR primers for storage protein genes Pro10.1-F 5' GTTGCATGCAGCTACAAGGCATGA 3' Pro10.1-R 5' TCTGCATCGCCATCTTCACCATCT 3' Pro13a.2-F 5' TTCAGGCGATTGTGCAGCAACTAC 3' Pro13a.2-R 5' GGATGGCAAGTTTAAGGCCAGCAA 3' RT-qPCR primers for internal control Pro13b.2-F 5' GTATAGCATTGCGGCAAGCACCTT 3' Pro13b.2-R 5' ATGGCCTGGACAACGTTAATGTCC 3' Pro13b.18-F 5' ACATTGTTCAGGCCATAGTGCAGC 3' Pro13b.18-R 5' ACAGAGCTTGAGCTTGAGCCAGAT 3' Pro16.2-F 5' GCTCTCAATTTGCCCTCCATGTGT 3' Pro16.2-R 5' TGGTACACACTACCAAGAACCGCA 3' α-globulin-f 5' GATCAGTTCATCACCAACAAAACA 3' α-globulin-r 5' TCCAGAACGCACAAAATCAT 3' Ubiquitin-F 5' TCTTCCCCTTCAGGACGTGT 3' Ubiquitin-R 5' GGACCAAAGGTCACCACCAT 3' 15
16 RT-qPCR primers for GPA4 and its homologous genes GPA4 cdna cloning GPA4 gdna cloning Membrane protein verification Subcellular localization GPA4-F 5' TGTTCTGCTCTTCACTGGTGTGGT 3' GPA4-R 5' AGCCTAGCCCAATCTTCTTGAGGT 3' Os10g F 5' ATTGGGATAGGGCTGACAGGCTTT 3' Os10g R 5' GCCTGACAGGAAGAGAATATTGCCCA 3' Os02g F 5' TGCTTGGTTGGCAATCTATGTGGC 3' Os02g R 5' ACAGGCCATCGGACAAAGAGAAGA 3' GPA4-cDNA-F 5' GAAAACGCCGCTACGCTACCAC 3' GPA4-cDNA-R 5' GCTCAAGCATAAGCCGACAAGG 3' GPA4-gDNA-F 5' GCACCTAACTGTTTCACTTTCTG 3' GPA4-gDNA-R 5' CCTCTACTGCTACCTTGTCCC 3' 1305GOT1B-F 5' TCTAGAATGGTTTCCTTCGAGATGAA 3' 1305GOT1B-R 5' AGATCTGCTCAAGCATAAGCCGACAA 3' 1305OsTip3-1-F 5' TCTAGAATGAGCACGGCGGCGGCGA 3' 1305OsTip3-1-R 5' AGATCTCTAGTAGTCCTCCGGCGC 3' 1305GFP-GOT1B 5' TGGACGAGCTGTACAGATCTATGGTTTCCTTCGAGATG -F 3' 1305GFP-GOT1B 5' GGGCGGCCGCTTTAAGATCTCTACACTGGAACCCGTTT -R 3' 1305-GOT1B-GF P-F 5' TCTAGAATGGTTTCCTTCGAGATG 3' 1305-GOT1B-GF P-R 5' GGATCCCACTGGAACCCGTTTTCC 3' pgbt9-got1b-f 5' GAATTCATGGTTTCCTTCGAGATGA 3' pgbt9-got1b-r 5' GGATCCGCTCAAGCATAAGCCGACAA 3' pgadsec12b-f 5' GGAGGCCAGTGAATTCATGGCGCGGCGCGGCGGC 3' pgadsec12b-r 5' CACCCGGGTGGAATTCTCAATGTAGAAACCTTGC 3' pgadsar1b-f 5 GAATTCATGTTCCTGTGGGACTGG 3 PGADSar1b-R 5 GGATCCCTACTTGATGTACTGGGA 3 pgadsar1c-f 5' GAATTCATGTCGTTTCTGCTGGAT 3' Y2H assay pgadsar1c-f 5' GGATCCCTACTTGATGTACTGTGA 3' pgadsec13a-f 5' GGAGGCCAGTGAATTCATGCCTCCCCATAAGATA 3' pgadsec13a-r 5' CACCCGGGTGGAATTCCTAAGCCTCAACAGTGGT 3' pgadsec13b-f 5' GGAGGCCAGTGAATTCATGTCGTCAAACAAGATA 3' pgadsec13b-r 5' CACCCGGGTGGAATTCCTATGGCTCCACCTTGGT 3' pgadsec13c-f 5' GGAGGCCAGTGAATTCATGTCATCGAAGAAAATA 3' pgadsec13c-r 5' CACCCGGGTGGAATTCCTACTGCTCAGCCTTCTT 3' pgadsec23a-f 5' GGAGGCCAGTGAATTCATGGAGGAGACGTCGACG 3' pgadsec23a-r 5' CACCCGGGTGGAATTCTTAGCTTGGTTCAGGTGG 3' 16
17 pgadsec23b-f 5' GGAGGCCAGTGAATTCATGTCTGAGTTCCTGGAT 3' pgadsec23c-r 5' CACCCGGGTGGAATTCCTACTGAACTGCCAGCCG 3' pgadsec23c-f 5' GGAGGCCAGTGAATTCATGGCGGCCGATCCCTCC 3' pgadsec23c-r 5' CACCCGGGTGGAATTCTTAGGACTGAACGGCCAG 3' pgadsec24-f 5' GGAGGCCAGTGAATTCATGCAACCACCGATGAGA 3' pgadsec24-r 5' CACCCGGGTGGAATTCTCACGTCATCTTGCTCTG 3' pgadsec31-f 5' GGAGGCCAGTGAATTCATGATCAACTCCGAGGGG 3' pgadsec31-r 5' CACCCGGGTGGAATTCTTACATTCTGAAATTCTG 3' pgbt9-got1p-f 5' ACTGTATCGCCGGAATTCATGTGGCTCACAGAGGCT 3' pgbt9-got1p-r 5' ACGGATCCCCGGGAATTCTTATACTGGCAGAACCCT 3' pgbt9-hgot1a- F 5' ACTGTATCGCCGGAATTCATGATCTCCATCACCGAA 3' pgbt9-hgot1a- R 5' ACGGATCCCCGGGAATTCTCAGACCATCGAGCTAGT3' pgbt9-hgot1b- F 5' ACTGTATCGCCGGAATTCATGATCTCCTTAACGGAC 3' pgbt9-hgot1b- R 5' ACGGATCCCCGGGAATTCTTATACCATATTGTTGCT 3' 5' pgadsec23p-f ATGGAGGCCAGTGAATTCATGGACTTCGAGACTAATGA3' 5' CCCACCCGGGTGGAATTCCTATGCCTGACCAGAGACGG pgadsec23p-r 3' pgadhsec23b-f 5' ATGGAGGCCAGTGAATTCATGGCGACATACCTGGAG 3' pgadhsec23b-r 5' CCCACCCGGGTGGAATTCTTAACAGGCACTGGAGAC 3' pbt3n-got1b-f 5 GCCGCCTCGGCCCCATGGATGGTTTCCTTCGAGATG 3 pbt3n-got1b-r 5 GTTAGCTACTTACCATGGCTACACTGGAACCCGTTT 3 5 TTCCAGATTACGCTGGATCCATGGAGGAGACGTCGACG ppr3n-sec23a-f 3 5 TGATACCACTGCTTGGATCCTTAGCTTGGTTCAGGTGG ppr3n-sec23a-r 3 ppr3n-sec23b-f 5 TTCCAGATTACGCTGGATCCATGTCTGAGTTCCTGGAT 3 5 TGATACCACTGCTTGGATCCCTACTGAACTGCCAGCCG ppr3n-sec23b-r 3 5 TTCCAGATTACGCTGGATCCATGGCGGCCGATCCCTCC ppr3n-sec23c-f 3 5 TGATACCACTGCTTGGATCCTTAGGACTGAACGGCCAG ppr3n-sec23c-r 3 qpcr primers for OsSec23 genes OsSec23a-F 5' TGGCAAGTGTGCAGGATGATGTTG 3' OsSec23a-R 5' ATAGCATCTGAGGCAGTTCTGGCA 3' OsSec23b-F 5' GATCAGCAAGGGCATGAGGCATTT 3' 17
18 OsSec23b-R 5' TGATCACACACGACCAAACGAGGA 3' OsSec23c-F 5' AGGACGATTTCCTGCTCCAAGACT 3' OsSec23c-R 5' ACCTGGAGGAACATCATGTGCTGA 3' Y N -GOT1B-F 5' GAAGGGAGGTACTAGTATGGTTTCCTTCGAGATGAA 3' Y N -GOT1B-R 5' TAGAAGCCATACTAGTCTACACTGGAACCCGTTTT 3' Y C -OsSec23c-F 5' CAAGGGCGGTACTAGTATGGCGGCCGATCCCTCC 3' BiFC assay Y C -OsSec23c-R 5' TAGAAGCCATACTAGTTTAGGACTGAACGGCCAG 3' Y C -OsSec23a-F 5' CAAGGGCGGTACTAGTTTAGCTTGGTTCAGGTGG 3 Y C -OsSec23a-R 5' TAGAAGCCATACTAGTTTAGCTTGGTTCAGGTGG 3 Y C -OsSec23b-F 5' CAAGGGCGGTACTAGTATGTCTGAGTTCCTGGAT 3 Y C -OsSec23b-R 5' TAGAAGCCATACTAGTCTACTGAACTGCCAGCCG 3 18
PHT1;2-CFP YFP-PHF + PHT1;2-CFP YFP-PHF
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Supplemental Materials
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Supplemental Data. Wu et al. (2). Plant Cell..5/tpc RGLG Hormonal treatment H2O B RGLG µm ABA µm ACC µm GA Time (hours) µm µm MJ µm IA
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Supplemental Data. Aung et al. (2011). Plant Cell /tpc
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Supplementary Data Supplementary Figures
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Figure S1. DELLA Proteins Act as Positive Regulators to Mediate GA-Regulated Anthocyanin
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Supplementary Information
Supplementary Information MED18 interaction with distinct transcription factors regulates plant immunity, flowering time and responses to hormones Supplementary Figure 1. Diagram showing T-DNA insertion
Supplemental Figure 1. Alignment of the NbGAPC amino acid sequences with their Arabidopsis homologues.
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Supplementary Table 1. The Q-PCR primer sequence is summarized in the following table.
Supplementary Table 1. The Q-PCR primer sequence is summarized in the following table. Name Sequence (5-3 ) Application Flag-u ggactacaaggacgacgatgac Shared upstream primer for all the amplifications of
ATL1-GFP Marker-mCherry/RFP Merge B C D
Input IP:α-H EDR1-H stedr1-h EV EDR1-H stedr1-h EV α-h α-mcherry TL1-GFP Marker-mCherry/RFP Merge C D GmMan49-mCherry mcherry-syp21 VH-a1-RFP E F G H I J EDR1-nYFP+TL1-cYFP ra6-mcherry Merge K L M Supplemental
Supplementary information
Supplementary information Supplementary figures Figure S1 Level of mycdet1 protein in DET1 OE-1, OE-2 and OE-3 transgenic lines. Total protein extract from wild type Col0, det1-1 mutant and DET1 OE lines
To investigate the heredity of the WFP gene, we selected plants that were homozygous
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Supplementary Figure 1 Collision-induced dissociation (CID) mass spectra of peptides from PPK1, PPK2, PPK3 and PPK4 respectively.
Supplementary Figure 1 lision-induced dissociation (CID) mass spectra of peptides from PPK1, PPK, PPK3 and PPK respectively. % of nuclei with signal / field a 5 c ppif3:gus pppk1:gus 0 35 30 5 0 15 10
Supporting Information
Supporting Information Üstün and Börnke, 2015 Figure S1: XopJ does not display acetyltransferase activity. Autoacetylation activity in vitro. Acetylation reactions using MBP, MBP-XopJ, GST and GST-HopZ1a
Supplementary data. sienigma. F-Enigma F-EnigmaSM. a-p53
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Nature Structural & Molecular Biology: doi: /nsmb.3308
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Supplemental materials
Supplemental materials Materials and methods for supplemental figures Yeast two-hybrid assays TAP46-PP2Ac interactions I. The TAP46 was used as the bait and the full-length cdnas of the five C subunits
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Supplemental Figure 1. Protein alignment of with. Identical aligned residues highlighted in black and similar and non-similar residues highlighted in grey and white, respectively. Position of Trp residues
Supplemental Data. Hu et al. Plant Cell (2017) /tpc
1 2 3 4 Supplemental Figure 1. DNA gel blot analysis of homozygous transgenic plants. (Supports Figure 1.) 5 6 7 8 Rice genomic DNA was digested with the restriction enzymes EcoRⅠ and BamHⅠ. Lanes in the
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α-mannosidase II (HGL1/MANII) β1,2-n-acetyl-glucosaminyltransferase II (GNTII)
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Supplementary Information
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Gene specific primers. Left border primer (638) ala3-4 (Salk_082157) Gene specific primers. Left border primer (1343)
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Supplemental Data. Steiner et al. Plant Cell. (2012) /tpc
Supplemental Figure 1. SPY does not interact with free GST. Invitro pull-down assay using E. coli-expressed MBP-SPY and GST, GST-TCP14 and GST-TCP15. MBP-SPY was used as bait and incubated with equal amount
Supplemental Data. Liu et al. (2013). Plant Cell /tpc
Supplemental Figure 1. The GFP Tag Does Not Disturb the Physiological Functions of WDL3. (A) RT-PCR analysis of WDL3 expression in wild-type, WDL3-GFP, and WDL3 (without the GFP tag) transgenic seedlings.
PIE1 ARP6 SWC6 KU70 ARP6 PIE1. HSA SNF2_N HELICc SANT. pie1-3 A1,A2 K1,K2 K1,K3 K3,LB2 A3, A4 A3,LB1 A1,A2 K1,K2 K1,K3. swc6-1 A3,A4.
A B N-terminal SWC2 H2A.Z SWC6 ARP6 PIE1 HSA SNF2_N HELICc SANT C pie1-3 D PIE1 ARP6 5 Kb A1 200 bp A3 A2 LB1 arp6-3 A4 E A1,A2 A3, A4 A3,LB1 K1,K2 K1,K3 K3,LB2 SWC6 swc6-1 A1,A2 A3,A4 K1,K2 K1,K3 100
(phosphatase tensin) domain is shown in dark gray, the FH1 domain in black, and the
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Myc- HA-Grb2 Mr(K) 105 IP HA 75 25 105 1-1163 1-595 - + - + - + 1164-1989 Blot Myc HA total lysate 75 25 Myc HA Supplementary Figure S1. N-terminal fragments of bind to Grb2. COS7 cells were cotransfected
Supplemental Figure 1
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sides of the aleurone (Al) but it is excluded from the basal endosperm transfer layer
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S156AT168AY175A (AAA) were purified as GST-fusion proteins and incubated with GSTfused
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Supplemental Materials Supplemental Figure S1 (a) Phenotype of the wild type and grik1-2 grik2-1 plants after 8 days in darkness.
Supplemental Data. Zhang et al. (2013). Plant Cell /tpc
SDLTXgal SDLTH SDLTHA ADSV4 BD BDP53 BD BDP53 BD BDP53 ADAt1g1567(aa36358) ADAt1g844(aa9354) ADAt1g844(aa51354) ADAt1g844(full) Prey ADAt1g844(aa11354) ADAt3g5994(aa48418) BD BDPAL2 BD BDPAL2 BD BDPAL2
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DOI:.38/ncb327 a b Sequence coverage (%) 4 3 2 IP: -GFP isoform IP: GFP IP: -GFP IP: GFP Sequence coverage (%) 4 3 2 IP: -GFP IP: GFP 33 52 58 isoform 2 33 49 47 IP: Control IP: Peptide Sequence Start
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doi:10.1038/nature11070 Supplementary Figure 1 Purification of FLAG-tagged proteins. a, Purification of FLAG-RNF12 by FLAG-affinity from nuclear extracts of wild-type (WT) and two FLAG- RNF12 transgenic
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transcription and the promoter occupancy of Smad proteins. (A) HepG2 cells were co-transfected with the wwp-luc reporter, and FLAG-tagged FHL1,
Supplementary Data Supplementary Figure Legends Supplementary Figure 1 FHL-mediated TGFβ-responsive reporter transcription and the promoter occupancy of Smad proteins. (A) HepG2 cells were co-transfected
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DOI:.38/ncb54 sensitivity (+/-) 3 det- WS bri-5 BL (nm) bzr-dbri- bzr-d/ bri- g h i a b c d e f Hypcocotyl length(cm) 5 5 PAC Hypocotyl length (cm)..8..4. 5 5 PPZ - - + + + + - + - + - + PPZ - - + + +
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Figure S1 is related to Figure 1B, showing more details of outer segment of
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Institute of Biological Chemistry, Washington State University, Pullman, Washington, USA
Plant and Cell Physiology Advance Access published July 15, 2009 Running title: The glutelin RNA ER zip code signals Name and address for editorial corresponding: Thomas W Okita Institute of Biological
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki , Japan b
This article is a Plant Cell Advance Online Publication. The date of its first appearance online is the official date of publication. The article has been edited and the authors have corrected proofs,
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Supplementary Methods Protein expression and in vitro binding studies. Recombinant baculovirus carrying GST, GST-mCC, GST-mSec, GST-mSecA and GST-mSecD were generated according to the manufacturer s instructions
monoclonal antibody. (a) The specificity of the anti-rhbdd1 monoclonal antibody was examined in
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