Evaluation of the Impact of E. coli Isolation Method on BST Results
|
|
- Benedict Clark
- 6 years ago
- Views:
Transcription
1 Evaluatin f the Impact f E. cli Islatin Methd n BST Results Emily Martin Sil & Aquatic Micrbilgy Labratry Department f Sil & Crp Sciences Texas A&M University 2012 Land Grant and Sea Grant Natinal Water Cnference Bacterial Detectin and Tracking Sympsium May 22, 2012 Imprving Life thrugh Science and Technlgy.
2 Bacteria #1 Cause f Water Quality Impairment in Texas
3 What is Bacterial Surce Tracking (BST)? Sme members f gut cmmunities can be hst specific. Explit specificity t track fecal cntaminatin back t surce. Target indicatrs because f their link t regulatry standards Varius appraches, but nt ne universally accepted
4 Gentypic BST Methds - Library-Dependent Fingerprinting Enterbacterial repetitive intergenic cnsensus sequence-plymerase chain reactin (ERIC-PCR) RibPrinting (RP)
5 Texas State BST E. cli Library Database f Knwn-Surce Fecal Islates Dual-Fingerprints frm Texas BST watershed prjects (Starting in 2006) Dr. DiGivanni, UT Schl f Public Health Current Versin Islates frm 1285 Different Fecal Samples Knwn-Surce Samples frm each watershed Best-Match Apprach (80% Similarity) t Majr Surce Class Human, Livestck and Pets, and Wildlife
6 Texas State BST E. cli Library Surce Class (6-Way Split) Number f Islates Number f Samples Library Cmpsitin and Expected Randm Rate f Calculated Rate f Crrect Classificatin (RCC) Left Unidentified (unique patterns) Crrect Classificatin Human % 89% 19% Pets % 62% 32% Avian Livestck % 70% 25% Nn-Avian Livestck % 80% 11% Avian Wildlife % 80% 20% Nn-Avian Wildlife % 75% 15% Overall RARCC * = 17% ARCC = 79% 18%
7 EPA Apprved Enumeratin Methds: Cunts vs Eclgy Membrane Filtratin using Chrmgenic Media EPA Methd Mdified Membrane Thermtlerant E. cli (mtec) E. cli nly Texas BST E. cli Library EPA Methd 1604 Ttal Clifrms and E. cli (MI) Defined Substrate Technlgy - MPN Clilert System
8 Enumeratin Methd Cmparisns Hamiltn et al. (2005)
9 Culture-Independent Analysis f Clilert E. cli Psitive Wells - Nn-target taxa made up a substantial percentage f cmmunity, especially in creek water (L). - Klebsiella sp. enriched in sewage sample (R) % false psitive rates fr E. cli in creek water. Sercu et al. (2011)
10 Cmparisn f the Diversity f E. cli Islates using Different Enumeratin Methds BST Implicatins Objective: Evaluate diversity f E. cli cmmunities islated using varius EPA apprved methds and assess implicatins fr BST Hypthesis: Cmmunities frm varius methds will select fr different ppulatins and thus bias BST results Prblem: Very few labs acrss TX accredited fr EPA Methd 1603 Sampling Lcatins Burtn Creek Cllege Statin Attyac Bayu Big Irn Ore Creek Nacgdches Little Brazs River Campbells Creek Hearne Welder Wildlife Refuge Mdy Creek Sintn Plum Creek Lckhart White Oak Bayu Hustn
11 Materials and Methds 3 Media Types EPA Methd 1603 n mtec EPA Methd 1604 n MI Clilert Enumerate and Islate E. cli 45 islates per media type/lcatin Cnfirmed using EPA Methd 1603 verificatin prcedures Fingerprint via ERIC-PCR and RibPrinting Fingerprint patterns analyzed with BiNumerics sftware Bidiversity Indices ID using the Texas BST E.cli Library 3-way Split at 80% Similarity
12 E. cli Enumeratin Fur Lcatins
13 ERIC-RP Cmmunity Diversity A Shannn Weiner Diversity Index mtec MI Clilert Burtn Creek Big Irn Ore Creek Campbells Creek Mdy Creek White Oak Bayu Plum Creek B Shannn-Weiner Diversity Index Highest diversity f islates were btained with mtec (A) and MI Clilert selected fr the lease diverse ppulatins (B)
14 ERIC-RP Similarity Analysis Overall Cmmunity Overlap
15 ERIC-RP Similarity Analysis Overall Cmmunity Overlap Big Irn Ore Creek Mdy Creek White Oak Bayu
16 ERIC-RP Similarity Analysis Overall Cmmunity Overlap Burtn Creek Big Irn Ore Creek Campbells Creek Mdy Creek White Oak Bayu Plum Creek % Ttal Islates (n=45/site) mtec 18% 17% 7% 2% 13% 11% MI 15% 16% 16% 2% 7% 20% Clilert 2% 11% 9% 2% 13% 13% mtec + MI 9% 16% 4% 7% 5% 0% mtec + Clilert 7% 13% 24% 0% 0% 0% MI + Clilert 20% 11% 13% 0% 9% 0% mtec + MI + Clilert 29% 16% 27% 87% 53% 56% Mdy Creek Least Diverse Overall average n all three media at 5 ther sites 36% Lack f verlap = Selectin fr different cmmunities
17 3-Way Split ID acrss all Watersheds 3-way ID split: Humans, Dmesticated Animals, and Wildlife Cmparisn back t mtec Library Standard MI: WILD Clilert: DOM, WILD MI & Clilert: UNID
18 Cmparisn f Surce Classificatins Extreme variatin acrss watersheds N cnsistent trend acrss knwn-surce class r watershed Appears nt pssible t use ther enumeratin techniques (MI and Clilert) as basis fr existing BST librarydependent wrk
19 Cnclusins Enumeratin results were variable acrss sites Islatin media had a substantial impact n E. cli cmmunity cmpsitin Analysis suggests need fr standardizatin amng fecal indicatr enumeratin and islatin techniques t be used in bacterial surce tracking applicatins
20 Questins Emily Martin Texas A&M University Cllege Statin, TX Phne: (979) Funding Prvided by Texas State Sil and Water Cnservatin Bard
2014 Water Quality Report for Harbor Beach Water Treatment Plant
This reprt cvers the drinking water quality fr the Harbr Beach Water Treatment Plant fr the calendar year 2014. This infrmatin is a snapsht f the quality f the water prvided t yu in 2014. Included are
More informationFirst Things First. Awkward. Backflow Prevention & Residential Landscape. 17 Failure Rates of Assemblies on Lawn Irrigation Systems Joe Smolinski
17 Failure Rates f Assemblies n Lawn Je Smlinski & Residential Landscape Ptential fr Failure & Assciated Risk First Things First First Things First Je Smlinski Currently: Deputy City Manager in Mansfield,
More informationRenewable Portfolio Standards: Costs and Benefits
Renewable Prtfli Standards: Csts and Benefits Lri Bird, NREL Renewable Energy Markets December 4, 2014 C-authrs: J. Heeter 1, G. Barbse 2, S. Weaver 2, F. Flres 1, K. Kuskva-Burns 1, and R. Wiser 2 1 Natinal
More informationPerformance Standards Framework for Science
Perfrmance Standards Framewrk fr Science Unit Organizer: (Apprximate Time: fur day perid) OVERVIEW: Tabby structures were very ppular during the late 1800 s. Peple used specific methds t build what are
More informationTAG Training Provider Program
Thank yu fr yur interest in becming a TAG Training Prvider. The prcess t becme part f this prgram is the fllwing: 1. Cmplete and submit this applicatin t Amanda Shk ashk@tagnline.rg 2. Upn invice r receipt
More informationIsetan Personal Data Protection Policy (PDPP)
Isetan Persnal Data Prtectin Plicy (PDPP) ISETAN OF JAPAN SDN BHD (IOJ) and ICJ DEPARTMENT STORE (M) SDN BHD (ICJ) are cmmitted t ensure that Custmer s privacy is prtected in accrdance t the Persnal Data
More informationIBM Global Services. Server Optimization ... Trends and Value Proposition That Can Drive Efficiencies and Help Businesses Gain A Competitive Edge
IBM Glbal Services Server Optimizatin.......... Trends and Value Prpsitin That Can Drive Efficiencies and Help Businesses Gain A Cmpetitive Edge Intrductin A typical rganizatin s success and ability t
More informationWelding. Course Design
Welding Curse Design 2007-2008 Curse Infrmatin Organizatin Eastern Arizna Cllege Divisin Industrial Technlgy Educatin Curse Number MSP 110 Title Welding Credits 2 Develped by Newell Dryden Lecture/Lab
More informationIMI2 PROPOSAL TEMPLATE FIRST STAGE PROPOSAL
IMI2 PROPOSAL TEMPLATE FIRST STAGE PROPOSAL IN TWO-STAGE PROCEDURE (TECHNICAL ANNEX) RESEARCH AND INNOVATION ACTIONS & INNOVATION ACTIONS Nte: This is fr infrmatin nly. The definitive template fr yur call
More informationVerification Summary Report (VSR) Instructions
Verificatin Summary Reprt (VSR) Instructins The verificatin prcess must be dne at the spnsr (district) level. Accessing the VSR Lg int IWAS; Click n System Listing n the left side f the screen; Click n
More informationB&B Food Distributors Data Synchronization Initiative
B&B Fd Distributrs Data Synchrnizatin Initiative Frequently Asked Questins fr B&B Fd Distributrs Suppliers Versin 1 Cpyright 2012. All Rights Reserved. FAQs fr B&B Fds Distributrs Suppliers REVISION HISTORY
More informationMarketing Summary Chapter 4
Marketing Summary Chapter 4 Marketing chapter 4 - Marketing Research: Gather, Analyze & Use Infrmatin Marketing Ethics: taking an ethical & abve-bard apprach t cnducting marketing research that des n harm
More informationHeat Pump KEYMARK. Date Description of modification Revision number Initial version 1
Page: 1 f 26 Date Descriptin f mdificatin Revisin number 14-12-2015 Initial versin 1 10/03/2017 Additin f air/air Editrial changes Annex E included in reference t pren12102-2 suppressed Reference t EN
More informationReport on Inspection of Grant Thornton LLP (Headquartered in Chicago, Illinois) Public Company Accounting Oversight Board
1666 K Street, N.W. Washingtn, DC 20006 Telephne: (202) 207-9100 Facsimile: (202) 862-8433 www.pcabus.rg Reprt n 2013 (Headquartered in Chicag, Illinis) Issued by the Public Cmpany Accunting Oversight
More informationTOWN OF PAYSON WATER DEPARTMENT BACKFLOW PREVENTION PROGRAM CUSTOMER INFORMATION PACKAGE
TOWN OF PAYSON WATER DEPARTMENT BACKFLOW PREVENTION PROGRAM CUSTOMER INFORMATION PACKAGE TOWN OF PAYSON Backflw Preventin Prgram Develpers, Cntractrs and Paysn Residents: An apprved backflw preventin assembly
More informationModule 4. Chapter 6 Microbial Growth
Mdule 4 Chapter 6 Micrbial Grwth Micrbial grwth refers t increase in, nt Grwing micrbes means an increase in Imprtant t understand cnditins necessary fr micrbial grwth The requirements fr grwth Physical
More informationProject Charter. Current 8/23/2006. Version 2
Prject Charter Current 8/23/2006 Versin 2 Dcument Cntrl Dcument Title: Authr(s): Prject Charter CMS Prject Team Date By Actin Versin Pages 8/23/06 SKC Template applied 1.0 All 9/13/06 KKee Updated TOC
More informationStanding Orders/Continuations in Voyager
Standing Orders/Cntinuatins in Vyager The primary issue f using the Vyager acquisitins client t rder and receive standing rders is the difficulty with the subscriptin pattern. Many standing rder vlumes
More informationLegend. Louth. Area 6. Meath DNW DNC. Area 5 DSW DL DSC DSW. Kildare/W Wicklow. Wicklow. Area 4. Wexford Tipperary SR
6.4.1 Optin 1 - Based n Hspital Grups This ptin sets ut a prpsal which suggests six successr Cmmunity Healthcare Organisatins t the existing ISAs (frm heren this shall be referred t as Optin 1 ). These
More informationUpdated: Mitigating Fraud Risk Through Card Data Verification
Risk Management Best Practices 30 June 2016 Updated: Mitigating Fraud Risk Thrugh Card Data Verificatin AP, Canada, CEMEA, LAC, U.S. Acquirers, Issuers, Prcessrs Overview: Visa is prviding issuers with
More informationProject document. HWTS Promotion in schools
Prject dcument HWTS Prmtin in schls Octber 2011 1 Prject title HWTS and Hygiene Prmtin in schls 2 Prject Backgrund The prject bjectives are t develp a target-grup riented training manual and t reach 9
More informationTippecanoe County Public Library Collection Management Policy
Tippecane Cunty Public Library Cllectin Management Plicy Purpse f Cllectin Management Plicy This plicy is established by the Library Bard t guide the library staff in the management f the cllectin and
More informationA Comparison of Different Age-of-Dam and Sex Correction Factors for Birth, Weaning and Yearling Weights in Beef Cattle
A Cmparisn f Different Age-f-Dam and Sex Crrectin Factrs fr Birth, Weaning and Yearling Weights in Beef Cattle Carla G. Chenette and R. R. Frahm Stry in Brief Preweaning perfrmance recrds f 2747 Angus
More informationNotes and guidance: Paper 1 Section B Poetic voices
Ntes and guidance: Paper 1 Sectin B Petic vices This resurce explains hw the questin in the specimen assessment materials fr AS Paper 1, Sectin B, Petic vices, addresses the assessment bjectives, with
More informationSeattle Public Schools The Office of Internal Audit
Seattle Public Schls The Office f Internal Audit Internal Audit Reprt September 1, 2013 thrugh August 31, 2016 Issue Date: June 13, 2017 Backgrund Infrmatin Executive Summary The District emplys apprximately
More information*************************************************************************************************************
* THIS PRINTED VERSION OF THE FORM IS FOR INFORMATION PURPOSES ONLY. * * YOU MUST COMPLETE AND SUBMIT THE FORM ONLINE AT * * GO.NCSU.EDU/SURVEY.REGISTRATION.FORM * NC State Survey Registratin Frm Intrductin
More informationDATE FOR COMING INTO EFFECT 1 April 2014*
Lndn, 24 March 2014 EMA/14522/2007-Rev.1 The Revised Checking Prcess f Mck-Ups and Specimens f uter/immediate labelling and package leaflets in the Centralised Prcedure fr Veterinary Medicinal Prducts
More informationThe Well Lifecycle & Data Management
The Well Lifecycle & Data Management PPDM April Lunchen Rbert Lvelace Rbert.Lvelace@sbti.cm 214-870-1235 4/22/13 Agenda Intrductin Well Lifecycle Overview Rle f Data Management Data management impacts
More informationNOAA/NSTA Symposium: Coral Ecosystems Thursday, March 27, 2008
NOAA/NSTA Sympsium: Cral Ecsystems NSTA Natinal Cnference n Science Educatin, Bstn, MA NOAA/NSTA Sympsium: Cral Ecsystems Thursday, 1:30 PM 1:55 PM Welcme, Intrductins, Gals fr the Sympsium Al Byers, Assistant
More informationCertificate in Construction Project Management
Certificate in Cnstructin Prject Management Duratin: 6 Mnths Distance Learning Prgramme Language: English RICS Member: 895.00 + VAT Nn RICS Member 1075.00 + VAT Curse Summary This prgramme explres the
More informationSolar Power: A Sustainable Energy System for Myanmar
Slar Pwer: A Sustainable Energy System fr Myanmar U Aung Myint General Secretary Renewable Energy Assciatin Myanmar (REAM) am.ream@gmail.cm First Myanmar-German Renewable Energies Sympsium 23 rd Octber
More informationOffshore Insights Help Guide Series. HELP GUIDE SERIES: Visiting Offshore Suppliers By neoit. June 2003 Issue 1.
Offshre Insights Help Guide Series www.neit.cm June 2003 Issue 1 HELP GUIDE SERIES: Visiting Offshre Suppliers By neit Page: 2 Intrductin Risks surrunding ffshre utsurcing must be understd and addressed
More informationU.S. Coast Guard Central Accounting & Reporting System (CARS)
U.S. Cast Guard Central Accunting & Reprting System (CARS) Agenda Cast Guard FBWT Backgrund Share Cast Guard s radmap fr success with transitining t CARS Audit challenges frm migratin Questins and Answers
More informationA method of securing cellular services information is being implemented under the
Cellular Telephne Service Descriptin The Cellular Telephne service plan prvides cellular telephne service and equipment frm a number f cellular telephne service prviders. The plan is intended t cver the
More informationDATA ON THE 4PS. WCAI Research Opportunity 27 September
DATA ON THE 4PS FOR A MULTI-CHANNEL/MULTI-BRAND RETAILER WCAI Research Opprtunity 27 September 2012 1 http://wcai.whartn.upenn.edu @WhartnCAI WHY SHOULD YOU SUBMIT A PROPOSAL? Access t unique data Simple,
More informationAn example of a Decision Support Framework for ecosystem-based fisheries management (MareFrame project)
An example f a Decisin Supprt Framewrk fr ecsystem-based fisheries management (MareFrame prject) Senir Scientist Petter Olsen, Nfima PrimeFish Stakehlder Wrkshp Vilanva i la Geltrú, Spain, April 6 th 2017
More informationPECO Innovation Challenge
2017-2018 PECO Innvatin Challenge Prgram Guidelines and Applicatin Apply NOW! Applicatins will be accepted and apprved n a rlling basis. All applicatins must be submitted by February 1. Fr mre details
More informationBusiness 59 Promoting Your Small Business
BUS 59 Syllabus Business 59 Prmting Yur Small Business Curse Overview and Syllabus Fall 2014 / 5 Units Curse Descriptin: This new curse fcuses n methds f prmtin fr small businesses. Students will examine
More informationEMPLOYEE INFORMATIONAL SESSION FAIR LABOR STANDARDS ACT (FLSA) CHANGES TO THE LAW
EMPLOYEE INFORMATIONAL SESSION FAIR LABOR STANDARDS ACT (FLSA) CHANGES TO THE LAW Presented by: Kathy Agnew Dir, Emplyment & Cmp Services 1 What Changed and Wh Changed It In May 2016, the U.S. Department
More informationJob Announcement Utah Climate Action Network Program Manager
Jb Annuncement Utah Climate Actin Netwrk Prgram Manager Overview The Utah Climate Actin Netwrk (the Netwrk) is seeking a dynamic individual t lead its grwth and success as a hub fr cllabratin and psitive
More informationFAST GUIDE TO PRICES AND ORDERING HOW TO CALCULATE PRICES AND PLACE AN ORDER
Page 1 f 6 HOW TO CALCULATE PRICES AND PLACE AN ORDER SERVICES Eurcircuits ffer 4 pling services, PCB prt, STANDARD pl, TECH pl and IMS pl, and 1 nn-pling service On demand. PCB prt: dedicated service
More informationTable of Contents. High Resolution Melt Software [HRM] v3.0.1 Release Notes
v3.0.1 Release Ntes Table f Cntents 1. OVERVIEW 2. FEATURES in HRM v3.0.1 3. SUSTAINED FEATURES 4. SYSTEM REQUIREMENTS 5. INSTALLATION OF THE THE SOFTWARE HRM V3.0.1 6. SUSTAINED KNOWN ISSUES 7. LEGAL
More informationGuidelines on how to prepare a winning CV
Guidelines n hw t prepare a winning CV A curriculum vitae (CV) is ften the first glimpse a wuld-be emplyer r fund-dnr will have f yu. It is als likely t be ne f many that wuld-be emplyers review. Therefre,
More informationIn this Document: EMV 3-D Secure General and Testing FAQs. EMV 3-D Secure General FAQs
In this Dcument: EMV 3-D Secure General and Testing FAQs 1. What is EMV 3-D Secure? EMV 3-D Secure General FAQs EMV Three-Dmain Secure (3DS) is a messaging prtcl develped by EMVC t enable cnsumers t authenticate
More informationUpon successful completion of this course, students will be able to meet the following objectives:
COURSE INFORMATION COURSE PREFIX/NO.: EVT 110 COURSE TITLE: INTRODUCTION TO TREATMENT FACILITIES LEC HRS/WK: 3.0 LAB HRS/WK: 0.0 CREDIT HRS/SEMESTER: 3.0 Distance Learning Attendance/VA Statement Textbk
More informationAnthropometric Analysis of Classroom Furniture Used in Colleges
Internatinal Jurnal f Engineering Research and Develpment e-issn: 2278-067X, p-issn: 2278-800X, www.ijerd.cm Vlume 3, Issue 10 (September 2012), PP. 01-07 Anthrpmetric Analysis f Classrm Furniture Used
More informationNGSS Reading Guide. Purpose. Reading. MS- LS2 Ecosystems: Interactions, Energy, and Dynamics
HO1 NGSS Reading Guide MS- LS2 Ecsystems: Interactins, Energy, and Reading Surces A Framewrk fr K- 12 Science Educatin: Practices, Crsscutting Cncepts, and Cre Ideas (2012) by NRC Next Generatin Science
More informationSABAL TRAIL PROJECT. RESOURCE REPORT 12 PCB Contamination. FERC Docket No. CP
SABAL TRAIL PROJECT RESOURCE REPORT 12 PCB Cntaminatin FERC Dcket N. CP15- -000 Nvember 2014 TABLE OF CONTENTS 12.0 RESOURCE REPORT 12 PCB CONTAMINATION... 12-1 12.1 INTRODUCTION... 12-1 Resurce Reprt
More informationDomain Architect Information Services (IS)
Dmain Architect Infrmatin Services (IS) Reprting t: Chief Architect Salary: 49,230-58,754 per annum (pr rata) depending n skills and experience. Salary prgressin beynd this scale is subject t perfrmance
More informationWITH EXAMPLES FROM THE WINDWARD ISLANDS. Andrew Bartlett. Keynote Presentation. 1st National Workshop on Development Communications.
PRINCIPLES OF EFFECTIVE DEVELOPMENT COMMUNICATION: WITH EXAMPLES FROM THE WINDWARD ISLANDS Andrew Bartlett Keynte Presentatin 1st Natinal Wrkshp n Develpment Cmmunicatins March 1986 Federal Agricultural
More informationReregistration of voluntarily deregistered CDM project activities
Paragraph 4 f the anntated agenda, Annex 1 Reregistratin f vluntarily deregistered CDM prject activities CDM EB 87 Paris, France, 23 27 Nvember 2015 UNFCCC Secretariat SDM prgramme Prcedural backgrund
More informationHow Safe Is Our Industry? The State of Tourism Security Society for Incentive Travel Excellence (SITE: Southern California)
Hw Safe Is Our Industry? The State f Turism Security 2017 TM Sciety fr Incentive Travel Excellence (SITE: Suthern Califrnia) July 2017 Dr. Peter Tarlw 1218 Merry Oaks, Cllege Statin, TX, 77840-2609 (+1-979)
More informationSAMPLE PROPOSAL. You are invited to submit a presentation proposal that addresses the Symposium theme:
SAMPLE PROPOSAL Yu are invited t submit a presentatin prpsal that addresses the Sympsium theme: "Sharing applicatins, success stries and lessns learned in reliability, durability and maintainability engineering."
More informationResults Day: Clearing Information and Apprenticeships. Thursday 17th August 2017
Results Day: Clearing Infrmatin and Apprenticeships Thursday 17th August 2017 RESULTS DAY: THURSDAY AUGUST 17 th 2017 Please read this infrmatin carefully If yu d nt get the results yu need t get int yur
More informationBest Practice Checklist for EPC
Best Practice Checklist fr EPC / A Definitins, interpretatin Technical aspect & what t lk fr in the EPC cntract 1. Is there a set f definitins f imprtant terms prvided and are thse clear and understd by
More informationApplication Portfolio Analysis: Tool for Cloud Migration Dr. Gopala Krishna Behera December 5, 2017
Applicatin Prtfli Analysis: Tl fr Clud Migratin Dr. Gpala Krishna Behera December 5, 2017 Tday, a majrity f custmers are getting ut f the data center business and mving twards the use f Clud Services.
More informationUDI Compliance Service
UDI Cmpliance Service What is UDI? The US Fd and Drug Administratin (FDA) has issued a ruling that medical devices must cnfrm t a Unique Device Identificatin (UDI) standard. The UK Department f Health
More informationMonitoring ICT developments Australia ICT Indicators Case Study
Internatinal Telecmmunicatin Unin Building Digital Bridges Sympsium Busan, Republic f Krea, September 10-11, 2004 Mnitring ICT develpments Australia ICT Indicatrs Case Study Vanessa Gray (vanessa.gray@itu.int)
More informationSovereign Industrial Capability Assessment Framework Update
Svereign Industrial Capability Assessment Framewrk Update Matt Ramage, Assistant Secretary Defence Industry 10 Octber 2017 Svereign Industrial Capability Assessment Framewrk Guidance Defence will evlve
More informationLNG as Fuel Operations. Lessons Learned Questions & Answers Chad Verret Executive Vice President Harvey Gulf International Marine
LNG as Fuel Operatins Lessns Learned Questins & Answers Chad Verret Executive Vice President Harvey Gulf Internatinal Marine Agenda Intrductin Cmpany Overview What t lk fr & lk ut fr frm an wner s perspective?
More informationA Case for Component Based Software Engineering. Maurizio Morisio, Michel Ezran and Colin Tully IEEE Transactions on Software Engineering, May 2003
A Case fr Cmpnent Based Sftware Engineering Success and Failure Factrs in Sftware Reuse Maurizi Mrisi, Michel Ezran and Clin Tully IEEE Transactins n Sftware Engineering, May 2003 Raihan Al-Ekram Schl
More informationName Phone Logo. W&RSETA W&RSETA
Occupatinal Qualificatin Dcument Occupatinal Cde Qualificatin Title NQF Level Lg 523101 Occupatinal Certificate: Checkut Operatr 2 Name Email Phne Lg Develpment Quality Partner W&RSETA imarrian@wrseta.rg.za
More informationHEALTHY START RAPID EVALUATION OF EARLY IMPACT ON BENEFICIARIES, HEALTH PROFESSIONALS, RETAILERS AND CONTRACTORS
Tavistck Institute HEALTHY START RAPID EVALUATION OF EARLY IMPACT ON BENEFICIARIES, HEALTH PROFESSIONALS, RETAILERS AND CONTRACTORS EXECUTIVE SUMMARY This reprt presents the findings frm the rapid evaluatin
More informationSpecial Report. Debunking. the Top. Eight Myths. in Your DSD Mobile. Security Strategy. Surrounding Small-Business. Warehouse Management Systems
Special Reprt Debunking the Tp in Yur DSD Mbile Security Strategy Surrunding Small-Business Eight Myths Warehuse Management Systems Intrductin The era f manual warehuse peratins is drawing t a clse with
More informationPackaging regulations
Packaging regulatins This guidance applies t yu if yu wn, manage r wrk fr a business that prduces, uses r handles packaging r packaging materials. Prducer Respnsibility Obligatins - What yu must d! If
More informationMEDICINES CONTROL COUNCIL
Oversight and Mnitring in Clinical Trials MEDICINES CONTROL COUNCIL OVERSIGHT AND MONITORING IN CLINICAL TRIALS This guideline assists spnsrs f clinical investigatins in develping mnitring strategies and
More information2018 CLEAN CAMPUS COMPETITION School Report Form
2018 CLEAN CAMPUS COMPETITION Schl Reprt Frm Please prvide the infrmatin belw alng with Student Reprt Frm t the judge(s): Name f schl: Phne: Cntact persn: First name: Last name: Student cntact: First name:
More informationExaminer Tip Sheet Independent Review
Examiner Tip Sheet Independent Review Welcme t Independent Review! The purpse f Independent Review is fr each team member t independently evaluate the applicant s prcesses and results by identifying areas
More informationFort McMurray Burned and Unburned Soil Samples
Frt McMurray Burned and Unburned Sil Samples The analysis in this dcument was cnducted using publically available test results frm sil samples cllected in the weeks fllwing the Frt McMurray wildfire. These
More informationCDM Plan Submission and Review Criteria Rules
Cnservatin First Framewrk LDC Tl Kit Final v4.0 January 17, 2018 CDM Plan Submissin and Review Rules 1) Purpse and definitins 1.1 These Rules set ut: a) the rles, respnsibilities and timing fr CDM Plan
More informationFrequently asked questions:
Frequently asked questins: Standardized pre/pst surveys fr grw grant results Versin 2.0 May 10, 2017 Cntents HelpDesk Cntact Infrmatin... 2 General Questins... 2 Why is OTF using these surveys?... 2 Wh
More informationEXHIBIT B, MODIFICATION PS-0006, CONTRACT GS-10F-0114T LABOR CATEGORY DESCRIPTIONS
Prgram Manager 871-1 Strategic Planning fr Technlgy Prgrams / Activity Electrical Engineering 871-2 Cncept Develpment and Requirements Analysis Electrical Engineering 871-3 System Design, Engineering and
More informationA Web-Based E-Testing System Supporting Test Quality Improvement
A Web-Based E-Testing System Supprting Test Quality Imprvement Gennar Cstaglila, Filmena Ferrucci, Vittri Fuccella Dipartiment di Matematica e Infrmatica Università degli Studi di Salern, Fiscian(SA),Italy
More informationBarnardo s Volunteering and Community Engagement Strategy
1 2 Barnard s Vlunteering and Cmmunity Engagement Strategy 2015-2020 Freward frm Chief Executive We have a strng traditin f engaging cmmunities t supprt what this great des. This began nearly 150 years
More informationCountry profiles: Iceland
Cuntry prfiles: Iceland Last updated: 26/06/2015 1 Implementatin f Tracking Systems 1.1 Electricity Disclsure Electricity disclsure in Iceland is implemented by Regulatin number 757/2012, Regulatin n disclsure
More informationo Mitigation measures are required to be identified. Soil
Mitigatin measures are required t be identified. Sil Pllutin f sil due t handling, use and strage f hazardus substances during cnstructin and peratin. The lss f available tp sil. Mitigatin measures are
More informationStandard Operating Procedure Secchi Depth
Standard Operating Prcedure revised January 8, 2017 Secchi Depth Page 1 f 5 Standard Operating Prcedure Secchi Depth Table f Cntents 1 POINT OF CONTACT... 1 2 OBJECTIVE... 1 3 DEFINITIONS AND ABBREVIATIONS...
More informationNZATD Education Trust Awards elearning Award Guidelines for Entrants
NZATD Educatin Trust Awards elearning Award Guidelines fr Entrants Fcus f Award NZATD intrduced this award t recgnise excellence in the design and implementatin f elearning initiatives within rganisatins.
More informationOpen House Fact Sheet
Open Huse Fact Sheet What is an Open Huse? An pen huse is an infrmal meeting prcess where participants begin by explring varius displays, r statins, related t the meeting purpse. Each statin has a knwledgeable
More informationAppendix C: Country Case Study Methodology
Appendix C: Cuntry Case Study Methdlgy Guiding Principles Cuntry cases fllw a multiple cuntry case study design, that is, fcus n three reginal clusters each with three sets f PPP prjects with distinct
More informationRestaurant Pro Express Mobile v1.0
One Blue Hill Plaza, 16th Flr, PO Bx 1546 Pearl River, NY 10965 1-800-PC-AMERICA, 1-800-722-6374 (Vice) 845-920-0800 (Fax) 845-920-0880 Restaurant Pr Express Mbile v1.0 Usage Guidelines & Best Practices
More informationInterstate 91 Viaduct Study MassDOT Contract No Public Involvement Plan
Interstate 91 Viaduct Study MassDOT Cntract N. 82617 Public Invlvement Plan Prject Overview and Gals The prtin f Interstate 91 (I-91) between State Street and the interchange with I-291 parallels the Cnnecticut
More informationMichigan Local Public Health Accreditation Program Tool 2018 MPR Indicator Guide Section VI: Onsite Wastewater Treatment Management
Michigan Lcal Public Health Accreditatin Prgram MPR 1 The lcal health department shall have a wastewater treatment regulatin capable f prtecting the public health legally adpted under enabling state legislatin.
More informationEnergy Savings A District Perspective
Energy Savings A District Perspective Ohi Schl Bards Assciatin Capital Cnference 2012 9:00 AM 10:15 AM 1 Energy Optimizers, USA Greg Smith, President, CEM, LEED AP, BD+C, CLEP, CEA Ohi K-12 Schl Fcus (Energy
More informationDigital Campaign Manager March 2018
Digital Campaign Manager March 2018 0 Smart Metering Cmmunicatins Bdy Limited (trading as Smart Energy GB) is a cmpany registered in England and Wales. Cmpany number 8455995. Registered ffice: 1 Alfred
More informationHCPS III Science Standards Addressed: SC.BS.3.1, SC.BS.3.2, SC.BS.3.3, G.4.2, & G.4.3
Kal Matters Owen S. Yamaguchi Knawaena High Schl HCPS III Science Standards Addressed: SC.BS.3.1, SC.BS.3.2, SC.BS.3.3, G.4.2, & G.4.3 Grade Level: 9-12th Prject Time Span: 1 Quarter T The Teacher: This
More informationAvailable online at Energy Procedia 1 (2009) (2008) GHGT-9
Available nline at www.sciencedirect.cm Energy Prcedia 1 (2009) (2008) 2857 2861 000 000 Energy Prcedia www.elsevier.cm/lcate/prcedia www.elsevier.cm/lcate/xxx GHGT-9 Implementing CCS in Eurpe: ZEP s Visin
More informationIn this fact sheet we answer the four most common questions new clients ask:
Wrking With Federal Prpsals Intrductin In this fact sheet we answer the fur mst cmmn questins new clients ask: What tasks d yu perfrm and what d yu need frm us? Hw d we use the Virtual War Rms? Hw much
More informationOP 1: Greenhouse Gas Emissions
OP 1: Greenhuse Gas Emissins 10 pints available A. Credit Ratinale This credit recgnizes institutins that have inventried their greenhuse gas (GHG) emissins and that have reduced their adjusted net Scpe
More informationInfectious Substance Packing and Shipping Quick Reference Guide
Infectius Substance Packing and Shipping Quick Reference Guide www.phlet.rg Public Health Labratry f East Texas On the campus f UT Health Nrtheast (UT Heath Science Center-Tyler) 11937 U.S. Hwy 271, Bldg
More informationIESBA Meeting (March 2013) Agenda Item
Agenda Item 7-A Backgrund Lng Assciatin f Senir Persnnel (Including Partner Rtatin) with an Audit Client Matters fr Cnsideratin 1. Paragraph 290.150 f the IESBA Cde f Ethics fr Prfessinal Accuntants (the
More informationINTRODUCTION. If additional information is needed, please contact The IIA Research Foundation:
INTRODUCTION The Institute f Internal Auditrs Research Fundatin TM (IIARF TM ) is the glbal leader in prviding research and knwledge resurces t enhance the internal audit prfessin. The IIARF cntinually
More informationGuidelines on Use of Electronic Data Collection in Censuses: Use of Handheld Devices and Internet
1 Guidelines n Use f Electrnic Data Cllectin in Censuses: Use f Handheld Devices and Internet Meryem Demirci United Natins Statistics Divisin Experiences, gd practices and challenges fr the Natinal Statistical
More informationFavorPrep. Plant Genomic DNA Extraction Mini Kit. User Manual. For Research Use Only. Cat. No.: FAPGK 001 (50 Preps) FAPGK (100 Preps) v.
FavrPrep TM Plant Genmic DNA Extractin Mini Kit User Manual Cat. N.: FAPGK 001 (50 Preps) FAPGK 001-1 (100 Preps) Fr Research Use Only v.0905 Intrductin Genmic DNA Mini Kit prvides a fast and simple methd
More information1. Why can I modify a purchase requisition although I set the changeability of the release indicator to '1' ('Cannot be changed')?
Nte Language: English Versin: 10 Validity: Valid Since 25.08.2009 Summary Symptm FAQ Release Strategy List f questins : 1. Why can I mdify a purchase requisitin althugh I set the changeability f the release
More informationSouthern California Dredged Material Management Team (SC-DMMT) April 29, 2009 Meeting Notes. Agency representatives participating via teleconference.
Suthern Califrnia Dredged Material Management Team (SC-DMMT) April 29, 2009 Meeting Ntes I. Participating Agencies/Attendees: a. Jrine Camppian (EPA) b. Jack Gregg (CCC) c. Mike Lyns (RWQCB - Regin 4)
More informationEmpowered Workgroups. A process area at Level 4: Predictable
Empwered Wrkgrups A prcess area at Level 4: Predictable Purpse Descriptin The purpse f Empwered Wrkgrups is t invest wrkgrups with the respnsibility and authrity fr determining hw t cnduct their business
More informationMonitoring, Evaluation and Adaptive Management Following INFFER Assessment (INFFER step 7)
Mnitring, Evaluatin and Adaptive Management Fllwing INFFER Assessment (INFFER step 7) www.inffer.rg David Pannell, Geff Park, April Curatl, Anna Rberts, Stephanie Spry, Sally Marsh Intrductin The Investment
More informationNJDEP PROPOSES TO CHANGE FLOOD HAZARD AREA CONTROL ACT RULES
NJDEP PROPOSES TO CHANGE FLOOD HAZARD AREA CONTROL ACT RULES NJDEP has prpsed far-reaching regulatins which culd effect stream encrachment wetlands and buffers alng streams and tidal areas. Key highlights
More information