Bioelectronics. A.G. Mestre, P.C. Inácio, Luís Alcácer, Fabio Biscarini Maria C. R. Medeiros and Henrique L. Gomes

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1 Lisboa, July, 4-6 Bioelectronics A.G. Mestre, P.C. Inácio, Luís Alcácer, Fabio Biscarini Maria C. R. Medeiros and Henrique L. Gomes BASIC SCIENCES AND ENABLING TECHNOLOGIES Organic Electronics- LX 2005, it - instituto de telecomunicações. Todos os direitos reservados.

2 Outline Motivation Bioelectronic systems in vitro Organ-on-chip Electrical activity of tumour Fundamental research on cell cultures Implantable bioelectronics. Implant for a spinal cord-injury Conclusions 2

3 Motivation To develop bidirectional sensing devices to interact with living cells and tissues. cell Bi-directional communication Organic semiconductors, biocompatible and biodegradable substrates Electronic Interface Flexible array of sensors, (Takao Someya group) 3

4 Sensing devices Polymer electrodes produced by inkjet printing Schematic diagram of the recording system. 4 Conventional MEA (gold electrodes on silicon) Sensing device connected with a commercial Petri-dish.

5 Organ-on-chip (a) (b) Zebrafish heart on (a) gold (b) conducting electrodes Small organs are important research tools in fundamental studies of drug discovery and safety pharmacology. 5

6 Organ-on-chip Embryoid body with autonomous cardiac contractile cells 6

7 Electrochemically gated field effect transistors(egfet) Gate Electrolyte Semiconductor Source Drain Cross section view of EGFET 7 7

8 Graphene based transistors Source Gate Drain In collaboration with: The International Iberian Nanotechnology Laboratory 8 8

9 Graphene based transistors Gate Drain Source Cardio cells In collaboration with: The International Iberian Nanotechnology Laboratory 9 9 Sanaz.Asgarifar@gmail.com

10 Cancer cells Glioma cells 10

11 Bioelectrical activity of cancer cells Addition of antibiotic 11

12 Bioelectrical activity of cancer cells Electrode with active cells Electrode with active cells S V (V 2 /Hz) Electrode with silent cells Bare electrode Bare electrode Frequency (Hz) 12

13 Bio-electrical Signals 13 13

14 Signal analysis 14 7/6/

15 Implantable devices Active Multifunctional Implantable Device (AMID) to treat Spinal Cord Injury (SCI). Flexible, conformable to the injury site Organic electronics and microfluidics integrated Biocompatible, largely biodegradable, life time tailored to 4-6 months 15 15

16 Nano-fibrous bacterial cellulose Bacterial Cellulose is produced by bacteria Gluconacetobacter sacchari After compression between plates at room temperature, the water is removed. 1 cm 16

17 PLGA Poly(lactic-co-glycolic acid) (mechanical and stress responses) Bending radius down to 80 µm Poly(lactic-co-glycolic acid) is a Copolymer which is used in a host of Food and Drug Administration (FDA) approved therapeutic owing to its biodegradability and biocompatibility 17

18 Active Multifunctional Implantable Device (AMID) Transistors 18

19 Active Multifunctional Implantable Device 19

20 Implanted AMID device in contusion SCI animal model (acute) 20 20

21 Demonstration of implanted device in contusion SCI animal model Schematic view of the surgical technique for the AMID implantation in vivo Implantation of the AMID in the mouse spinal cord 21 21

22 Conclusions Large area printed polymer electrodes are suited to fabricate organ-on-chip systems. Simple planar sensing devices can be fabricated in glass or in biocompatible substrates such as bacterial cellulose. We have demonstrated the applicability of these printed electrodes to measure in vitro a population of contractile cells(cardiomyocytes) and quasi-periodic oscillations in Glioma cells. A flexible biocompatible active multifunctional device was developed to repair spinal cord injury. 22

23 Acknowledgements Ana Mestre Qian chen Asal Kiazadeh Joana Canudo Pedro Inácio Profª Maria Medeiros Profª Leonor Cancela Prof. Fabio Biscarini (Unimore, Modena, Italy) Thank you for your attention! 23 Bologna (Italy) 8-10 June 2016