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1 Proteome Binder Workshop / DGMS: Affinity-Mass Spectrometry December 13 15, 2009 University of Konstanz Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Speakers M. Taussig M. Glocker T. Joos M. Przybylski M. Ueffing M. Bantscheff F. Herberg S. Muyldermans K. Compson Cambridge Rostock Tübingen Konstanz München Heidelberg Kassel Brussels Manchester
2 International Workshop: Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Organisers: M. Przybylski & M. Glocker Organising committee Michael Przybylski Stefan Slamnoiu Gabriela Paraschiv Laboratory of Analytical Chemistry & Biopolymer Structure Analysis University of Konstanz Universitätsstrasse Konstanz, Germany University of Konstanz Central Building A, lecture hall K503 (IBZ II) Begin: Sunday, Dec. 13 th, 5:00 pm End of Workshop: Tuesday, Dec. 15 th, 1:00 pm
3 General information Venue The international workshop Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis takes place at the University of Konstanz. All lectures are given in the Central Building A, lecture hall K503 (IBZ II). Accomodation Major hotels located downtown in walking distance from the train station are listed below. Please contact Aleksandra Hajnic by or phone phone ). Hotel Halm Bahnhofplatz Konstanz Tel.: Stadthotel Bruderturmgasse Konstanz Tel.: Hotel Barbarossa Obermarkt Konstanz Tel.: Parking There are four parking lots at the University Campus area which can be recognized by the given signs. Transportation You can reach the University with bus lines 9A and 9B from the main train station Konstanz. From Wollmatingen you can use the bus line 11 to get to the University, and from Litzelstetten and Dingeldorf the bus line 4 is available, with the bus stop Egg convenient to reach the University. Wardrobe On level B4 you can find a supervised wardrobe. However, we recommend you not to leave any valued articles unattended. Conference office The meeting desk will be located adjacent to K503, and will be open starting from Sunday, 4:00 pm. PC and internet In the lecture hall K503 a laptop with internet connection will be available. We ask you to bring your PowerPoint presentation on an USB flash drive, for easy loading on the presentation computer. Workshop dinner The speakers dinner will be on Monday, Dec. 14 th, at 8:00 pm, at the Restaurant Elefanten, Salmannsweilergasse 34, Konstanz.
4 Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Organisers Michael Przybylski (University of Konstanz) and Michael Glocker (Proteome Center Rostock) DGMS Fachgruppe Affinity-Mass Spectrometry Description Mass spectrometry has been established as a key tool in proteomics, and in the identification of epitopes recognized by antibodies of polypeptides and protein antigens, that are crucial as lead structures for vaccine development. However, the combination of mass spectrometry and bioaffinity methods has been hitherto explored and developed only to an small extent. In this workshop recent developments of Bioaffinity- mass spectrometry will be presented and discussed for structural identification and bioaffinity quantification in ligand binder interactions. Major topics: Antigenic determinant identification; affinity- proteomics; interaction mapping using chemical crosslinking; biopolymer interaction studies by online bioaffinity-mass spectrometry; new approaches for conformational interaction mapping by ion-mobility-ms; applications to biomedical and clinical studies. Brief theme presentations by scientists from different laboratories and manufacturers will be given, together with up-to-date reports of most recent applications. Participation of graduate and postgraduate students is especially encouraged. Programme themes Online bioaffinity-ms for biopolymer & ligand- binder interaction studies Antigenic determinant / epitope identification Immuno- MS in proteomics applications Protein-interaction mapping using photoreactive tags Antibody analysis in autoimmune diseases Affinity-MS for individualized medicine Ion-mobility-MS in biopolymer interaction analysis
5 ProteomeBinders Workshop / DGMS-Fachgruppe Affinity-MS December 13 15, University of Konstanz Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Programme Sunday, 13 th December, 2009 K 503 (IBZ II) 16:00 Registration 17:30 Welcome & Introduction M. Przybylski 17:45 Opening lecture Marius Üffing, Helmholtz- Zentrum München Affinity-based approaches to target protein networks on a quantitative scale 18:45 Welcome buffet & poster session
6 Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Programme - continued Monday, 14 th December, 2009 K 503 (IBZ II) Session A: New developments and methods of affinity-ms in proteomics Chair: M.O. Glocker 09:00 Friedrich Herberg, University of Kassel Development and application of low molecular weight binders for chemical proteomics 09:45 Markus Bantscheff, Cellzome AG, Heidelberg Chemical proteomics for characterisation of small molecule kinase inhibitors 10:30 Iuliana Susnea, University of Konstanz Immuno- proteomics in the identification of Chlamydia pneumoniae antigens 11:00 Coffee Break 11:30 Oliver Pötz, Thomas Joos, NMI Reutlingen TXP - a generic immunoaffinity based strategy for protein analysis 12:15 Corina Flangea, Alina Zamfir, INCDEMC Timisoara/RO New glycosylaminoglycan domanins revealed by mass spectrometry methods based on chip-nano-esi 13:00 Discussion 13:15 Lunch Session B: Biochemical and biomedical applications Chair: F. Herberg 14:30 Keith Compson, Waters SA, Manchester/UK New developments of high-performance ion mobility mass spectrometry The Synapt G2 15:15 Michael Przybylski, University of Konstanz Affinity-MS and ion-mobility- MS in studies of misfolding / aggregating proteins in neurodegeneration 16:00 Coffee Break
7 Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Programme - continued Session B: Biochemical and biomedical applications - continued 16:30 Ulrich Pecks, RWTH Aachen Preeclampsia: The current clinical situation and novel affinitybased developments for improved diagnosis Michael O. Glocker, Proteome Center Rostock Next generation autoimmune disease diagnostics - Mass spectrometric and peptide chip epitope analysis of autoantigens 18:00 Poster session & Refreshments 20:00 Speakers Dinner (Restaurant Elephanten, Konstanz) Tuesday, 15 th December, 2009 K 503 (IBZ II) Session C: Perspectives for affinity- MS and proteomics Chair: M. Przybylski 09:00 Serge Muyldermans, Vrije University Brussels/BE Fast generation of high-affinity single domain antibody fragments 09:45 Marcus Macht, Bruker Daltonik, Bremen Characterisation of monoclonal immunoglobulins by affinity-ms: Strategies & tools 10:30 Adrian Moise, University of Konstanz Affinity-MS approaches for identifying protein-carbohydrate interaction epitopes 11:00 Coffee Break 11:30 Ilse Dohnal, Karl Mechtler, Institute of Mol. Pathology, Wien/AU Rapid isolation of Yeast proteins for phosphorylation site analysis 12:15 Mike Taussig, Babraham Institute, Cambridge/UK Generation and application of protein microarrays 13:00 Final discussion / End of workshop
8 University of Konstanz ProteomeBinders Workshop / DGMS Fachgruppe Affinity-MS December 13 15, 2009 Bioaffinity- Mass Spectrometry in Life Science and Biomedical Analysis Please send the form by fax or to: Aleksandra Hajnic Tel Fax Sekretariat.Przybylski@uni-konstanz.de Name: Address: Registration (please check the corresponding box): regular 40,- student 20,- Registration fee includes welcome buffet on Sunday, Dec. 13 th Please transfer the registration fee to the following account: Bank: Sparkasse Bodensee Account number: Bank code number: Re: Affinity-MS (Foreign participants may pay the registration fee at the workshop office.) Accommodation I would like a hotel reservation Dec. 13 th 15 th : Hotel reservation (50 90 / night) no reservation (When making a choice, please delete the symbol and insert an X instead.)
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