A NATIONAL RESEARCH INFRASTRUCTURE FOR BIOLOGICAL IMAGING
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1 A NATIONAL RESEARCH INFRASTRUCTURE FOR BIOLOGICAL IMAGING 1
2 FOUNDATION WP1-5 Project handling WP7 - Access to Innovative Technologies ALM WP6 - Advanced Light Microscopy (ALM) WP11 - Data Storage and Analysis WP12-13 User Access and Training IBiSA Infrastructures Cellular imaging From molecules to whole organisms Super resolution, Dynamics, Interactions, Multiscale imaging Industrial partners Training & Dissemination TEACHING & DISSEMINATION RESEARCH & DEVELOPMENT R&D teams State of the art technologies Optique, Microscopy, Spectroscopy, Chemical probes, Image processing EquiPex National & International networks OPENING Academic & Industrial users Research applications In physiology, cellular biology, developmental biology, pathologies (neurology, immunology, cancer) Labex IBiSA CRITERIA 2
3 FRANCE-BIOIMAGING INFRASTRUCTURE 5 LOCAL NODES & 1 TRANSVERSAL NODE Paris Centre, Ile de France Sud, Bordeaux, Marseille, Montpellier! Inria (Rennes) / Institut Pasteur!! 9 IMAGING CORE FACILITIES IMACHEM INSTITUTIONAL PARTNERS CNRS - leading partner! Inria, INSERM,! Institut Curie, Institut Pasteur,! Ecole Normale Supérieure,! Ecole Polytechnique,! Universités Aix-Marseille, Bordeaux Segalen, Montpellier 1 et 2, Paris Descartes, Paris Diderot, Paris Sud! 3
4 Strategic level GOVERNANCE Execu've Board: Na+onal coordinator + Nodes coordinators Scien'fic Advisory Board Na'onal Advisory Commi6ee Execu+ve Board + 5 consulta+ve members: Representa)ves of Users commi6ee (2), industries (CNOP) & ins)tu)on (CNRS) + Project Manager Project management Industrial partnership Process Plan Landscape perimeter Working plan FBI website: Applica'on/Support to events Networking & Communica+on Support to events MiFoBio School ELMI mee+ng Oct 2012 May 2013 Assises du RTmfm EMBO Courses Nov 2013 BIAT 4 May 2013 Dec 2013
5 GOVERNANCE Opera.onal level Users Commi6ee Representa'ves of users commi6ees from nodes facili'es Bordeaux, Marseille, Montpellier, IdF Sud & Paris Centre (2) Representa've of Industry L Oréal Representa'ves of French microscopy networks GdR2588 RCCM RTmfm Work Packages R & D teams Access to innova.ve technologies Training & Technological transfert Users access FBI website: Applica'on/Scien'fic projects 5
6 5 WORK PACKAGES WP1 Instrumentation & Methods WG 1a: 2D & 3D Axial Super Resolution & Single Molecule Tracking WP2 High Throughput & High Content Screening WP3 Probe Development, Optomanipulation & Optogenetics WG 1b: Functional Imaging WG 1c: CLEM & Super CLEM WP4 Bioimage Informatics - Image Processing & Data Manipulation WP5 Training, Dissemination & Technological Transfer WG 1d: New Contrast & In- Depth Imaging 6
7 STRATEGY Do not reinvent the Wheel! STRENGTHEN CONNECTIONS WITH THE COMMUNITY OF BIOLOGICAL IMAGING Academic & industrial users! 7 7
8 EXPAND THE FRONTIERS OF KNOWLEDGE IN LIFE SCIENCES STRENGTHEN ATTRACTIVENESS AND COMPETITIVENESS OF FRANCE BRING TOGETHER RESEARCH AND INNOVATION SUSTAIN TRAINING AND DISSEMINATION - CONTACT@FRANCE-BIOIMAGING.ORG 50 nm FRANCE-BIOIMAGING.ORG ~ 8
9 TECHNOLOGIES & METHODS SUPER RESOLUTION IN LIGHT MICROSCOPY PhotoActivated Localization Microscopy (PALM), STochastic Optical Reconstruction Microscopy (STORM), Structured-Illumination Microscopy (SIM), STimulated- Emission-Depletion (STED)! MULTISCALE AND MULTIMODAL IMAGING Correlative Light-Electron Microscopy (CLEM) & SuperCLEM! SINGLE MOLECULE DYNAMICS & INTERACTIONS FLUORESCENCE SPECTROSCOPY Single Particule Tracking-PALM (SPT-PALM), Alternating- Laser Excitation-Fluorescence Resonance Energy Transfer (ALEX-FRET), Fluorescence-Lifetime Imaging Microscopy (FLIM), Fluorescence Anisotropy Imaging Microscopy (FAIM), Fluorescence Correlation Spectroscopy (FCS), Fluorescence Cross-Correlation Spectroscopy (FCCS)! LABEL & LABEL-FREE NEW CONTRASTS Second-Harmonic Generation (SHG) microscopy, Third- Harmonic Generation (THG) microscopy, Coherent Antistokes Raman Scattering (CARS) microscopy IN DEPTH OPTICAL IMAGING Two/Three-Photon Excitation (2/3-PE) microscopy, Optical Parametric Oscillator (OPO), Single Plane Illumination Microscopy (SPIM)! OPTOMANIPULATION & OPTOGENETICS Probes development!! BIOIMAGE INFORMATICS Image Processing and Data Manipulation HIGH THROUGHPUT & HIGH CONTENT SCREENING 9
10 Nodes specificity Marseille PF. Lenne New cell imaging modalities for developmental biology and immunology Bordeaux D. Choquet SuperCLEM for neuronal pathologies and for plant physiology Montpellier M. Nollmann New cell imaging modalities for genome function analysis Ile de France Sud N. Peyrièras New cell imaging modalities for morphogenesis analysis Paris Centre (J Salaméro, M Coppey-Moisan) Institut Curie J. Salaméro Institut Jacques Monod M. Coppey-Moisan ENS A. Triller ImagoPole-Pasteur S. Shorte Wave front engineering microscopy group (P5) Multi scale Multi organism imaging for cell biology and cancerology V. Emiliani Image Processing and Data Manipulation JC. Olivo-Marin (Pasteur), C. Kervrann (INRIA, Rennes) Image data manipulation Image and signal processing, analysis, quantification and modeling Institut Pasteur JC. Olivo-Marin INRIA (Rennes) C. Kervrann 10
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