NUE-CROPS - SP2 IMPROVING NITROGEN-USE EFFICIENCY IN MAIZE. Project meeting May
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1 NUE-CROPS - SP2 IMPROVING NITROGEN-USE EFFICIENCY IN MAIZE Project meeting May
2 Partners Participant No. Participant Organisation Name Country European Research Institutes/Universities 1 (Co-ord.) Newcastle University (UNEW) UK 2 Wageningen University and Research Centre (WUR) NL 3 University of Göttingen (UGöt) DE 4 AgroBio Institute (ABI) BG 5 University of Aarhus (UAa) DK 6 Research Institute for Organic Farming (FiBL) CH 7 Scottish Crop Research Institute (SCRI) UK 8 University of Düsseldorf (HHUD) DE Research Institute from an ICPC region 9 Chinese Academy of Agricultural Science (CAAS) CN University from an industrialised 3 rd country 10 Washington State University (WSU) US European plant breeding/seed production companies 11 Kleinwanzlebener Saatgut (KWS) DE 12 CPB-Twyford (CPBT) UK 13 Agrico (Agrico) NL Major Subcontractor Sabanci University (SUFEN) TR 2 Datum Bereich für Dokumentenkennung
3 Workplan SP2 IMPROVING NITROGEN-USE EFFICIENCY IN MAIZE (SP-manager: P. Westhoff) Identification of novel QTL for Nit-UE and WUE in SE-European, S-American and Chinese maize mapping populations Identification of genes and physiological/morphological root characteristics associated with QTL for Nit-UE Effect of mycorrhizal inoculation on N, P and water use efficiency in maize genotypes and association with candidate gene polymorphism Effect of different fertiliser input types and levels on performance, gene and protein expression patterns in maize genotypes with contrasting Nit-UE Chairpersons: P Westhoff C. Leifert T Presterl KWS P Westhof HHUD T Presterl KWS Bingqiang Zhao CAAS 3 Datum Bereich für Dokumentenkennung
4 Background: EUREKA Project CEREQUAL Genetic Variability for NUE Grain yield at high N (t/ha) 9,0 8,5 8,0 7,5 7,0 6,5 rp=0.44** rg= LSD 5% 6,0 3,5 4,0 4,5 5,0 5,5 6,0 Grain yield at low N (t/ha) Relationship between testcross grain yields under low versus high N supply of 49 S 4 maize lines, evaluated at 4 locations 4 Datum Bereich für Dokumentenkennung
5 Selection for NUE 12.5 Grain yield at high N levels [t/ha] HxT LSD5% LxT Testcrosses of a conventional elite line Test crosses of a low-input line SL x T TH x T Grain yield at low N level [t/ha] 5 Datum Bereich für Dokumentenkennung
6 QTL for NUE N level SL x T TH x T 720 DH-Lines 2 N-levels 4 locations Percenatge of explained variance [%] low high from SL from TH from TH from SL 2 years Chromosome 6 Datum Bereich für Dokumentenkennung
7 Genetic variability for drought tolerance Drought stress A x B C x B Irrigation 7 Datum Bereich für Dokumentenkennung
8 SP 2.1:Identification of novel QTL for Nit-UE and WUE in SE- European, S-American maize mapping populations Materials: Population 1: Population 2: Population 3: 200 DH lines derived from S-American population (KWS) 200 KWS elite lines from South-East Europe (KWS) 200 DH lines derived from drought tolerant mutant line crossed with initial parent line (ABI) Field evaluation 2009/2010: High and low N input: 1 loc. Southern France (Pop. 1, Pop. 2) 1 loc. in Hungary (Pop. 2) 1 loc. Italy (Pop. 1) High and low water: 1 loc in Rumania (Pop. 1, Pop.2) High and low N input / High and low water: 1 loc in North-Central Bulgaria (Pop. 3, loc.) Genotyping: 110 SSR marker 8 Datum Bereich für Dokumentenkennung
9 SP 2.3: Effect of mycorrhizal inoculation on N, P and water use efficiency in maize genotypes and association with candidate gene polymorphism Materials: Set 1 (N=100): Testcrosses of doubled-haploid lines derived from old flint landraces, elite checks Set 2 (N=100): Testcrosses of a set of representative elite lines Seed Treatment: Seed inoculation before planting with mycorrhizal spores Genotyping: High throughput sequencing of approx. 20 candidate genes 40 SSR marker for popultion structure 9 Datum Bereich für Dokumentenkennung
10 SP 2.3: Field Testing Field Testing 2009: Germany All testcrosses 2 locations with high and low N-levels 2 locations with potential drought stress (only set 2) At each location with and without mycorrhizal seed inoculation Löningen Ostbevern Bernburg Field Testing 2010/2011: Germany Selected testcrosses only (approx. 20) Factorial field experiment with high and low levels of N and water Grucking 10 Datum Bereich für Dokumentenkennung
11 Low Input field in Grucking 11 Datum Bereich für Dokumentenkennung
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