NATIONAL RENEWABLE ENERGY CENTRE. CENER Bioprocess Development Activities

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1 NATIONAL RENEWABLE ENERGY CENTRE CENER Bioprocess Development Activities

2 í n d i c e 1. Introduction to CENER 2. Overview of CENER Activities in Bioeconomy 3. CENER s Industrial research infrastructure for bioprocesses 4. Examples of R&D projects

3 1. Introduction to CENER

4 Mission and Vision 01 Introduction to CENER Vision To be a research centre of excellence in the renewable energies field with international outreach. Mission To generate knowledge in the renewable energy field and to transfer it to the industry in order to boost sustainable energy development. Areas Wind Biomass Solar Photovoltaic Solar Thermal Energy Grid Integration Energy in Building 4

5 Infrastructures 01 Introduction to CENER Headquarters Sarriguren Wind Test Laboratory Sangüesa Second Generation Biofuels Centre Aoiz Offices: Sevilla Experimental Wind Farm Alaiz Microgrid, Sangüesa

CENER in numbers 1. Introduction to CENER 21,2 M 200 100 M The annual 2013 budget is 21,2 million.

6 CENER in numbers 1. Introduction to CENER 21,2 M M The annual 2013 budget is 21,2 million. 60% self-financing 200 employees researchers, technical staff and support personnel Total investments ( ): > 100 million More than 200 customers in all five continents

7 CENER in numbers 1. Introduction to CENER International activities S. América N. América Europa África Asia Australia

8 2. Overview of CENER Activities in Bioeconomy

9 DEMO PILOT LAB 02 Activities and strategic research lines Activities: Biorefinery-Bioproducts-Circular economy RAW MATERIALS LIGNOCELLULOSIC BIOMASS Cereal straw Energy crops.. Forest residues Food industry residues Compost Black liquor Spent mushroom substrate Organic fraction MSW BIORESIDUES PRODUCTS BIOFUELS Bioethanol Biodiesel Power Pellets.. Biobuthanol Biocomposites. Biopesticides Bioplastics Biosurfactants BIOPRODUCTS 9

10 02 Biomass Department Strategic Research Lines 1. Biomass Torrefaction 2. Development of Pellets from alternative raw materials 3. Sustainability Studies 4. Development of Bioprocesses 5. Biomass gasification 10

11 02 Biomass Department Strategic Research Lines Development of Bioprocesses Optimization of pre-treatment/fractionation process for lignocellulosic materials Development and optimization of enzymatic and fermentative processes Scaling-up and validation of enzymatic and fermentative processes Development of processes for gas fermentation Lignin depolymerisation processes Development of bioprocesses from organic fraction of MSW Application of separation and purification technics to bioprocesses 11

12 3. CENER s Industrial research infrastructure for bioprocesses

3. CB2G-overview The Second Generation Biofuel Centre consist of a Process Development Units (PDUs) for bioproducts and advanced biofuels on a pilot scale level as an intermediate step towards the

13 3. CB2G-overview The Second Generation Biofuel Centre consist of a Process Development Units (PDUs) for bioproducts and advanced biofuels on a pilot scale level as an intermediate step towards the industrial scale-up of these technologies The CB2G constitutes an integrated trial and demonstration platform designed to develop: processes, equipment and specific components, new bioproducts and biofuels bio-refinery concepts. 13

14 3. CENER s Industrial research infrastructures for bioprocesses Process Units Pretreatment Unit: Capacity 2MWt Kg Biomass/h Chipping for woody biomass. Chopping for herbaceous biomass Drying Torrefaction Milling. Pelletizing In operation from 2008 Gasification Unit: Capacity 2 MWt-500 Kg Biomass/h Gasification Island Thermal Oxidizer and Flue gases treatment In operation from 2012 Biochemical Process Unit: Capacity: Up to kg biomass / week Pretreatment. Solid / Liquid Fractionation Biological Processes (Enzymatic Hydrolysis & fermentation). In operation from

Pretreatment Enzymatic Hydrolysis Fermentation

5bar & 200ºC Stirred tank reactor (200 l) able to

15 3. CENER s Industrial research infrastructures for bioprocesses Biochemical Process Unit Pilot Plant Pretreatment Enzymatic Hydrolysis Fermentation Continuous horizontal reactor: 5Kg/h up to 14.5bar & 200ºC Stirred tank reactor (200 l) able to develop high consistency EH Bioreactors fully monitored. (40 l and 100 l) 15

16 3. CENER s Industrial research infrastructures for bioprocesses Biochemical Process Unit Industrial Plant Main equipment includes: Enzymatic hydrolysis (2 x 3 m 3 ) Biorreactors (1, 3 and 6 m 3 ) 16

17 4. Examples of R&D projects

18 4. UPSCALLING OF A FERMENTATION PROCESS UPSCALLING OF A FERMENTATION PROCESS (CONFIDENTIAL) Private customer: 2013 Project objetive: Scaling up of a fermentation process of conversion of wheat straw into industrial oils, including: pretreatment, enzymatic hydrolysis, filtration, fermentation, centrifugation and drying Detail of one of the fermenters in the Biochemical Process Unit CENER s main tasks. Project coordination: including equipment from vendors (plate filter, centrifuge, drier) Plant operation 3 test campaigns (2 x 3000 litter enzymatic hydrolysis, 3000 litter fermentation)

4. BIOREFINERY IN NAVARRE BIOREFINERY IN NAVARRE Navarre Government : 2014-2015 Coordinator: CENER... Partners: INTIASA, CNTA, UPNa-QA, AIN, UPNa-GILT Total budget: 0,98 M. Budget Cener: 0,15 M.

19 4. BIOREFINERY IN NAVARRE BIOREFINERY IN NAVARRE Navarre Government : Coordinator: CENER... Partners: INTIASA, CNTA, UPNa-QA, AIN, UPNa-GILT Total budget: 0,98 M. Budget Cener: 0,15 M. Project objetive: Developing a biorefinery concept design based on the sugar platform using domestic feedstocks from Navarre Lignocellulosic biomass pretreatment pilot plant CENER s main tasks. Project coordination Optimization of fractionation process at laboratoryscale Upscaling fractionation process to pilot plant scale

4. SYN2OL SYN2OL Funded by Navarre Government, 2015-2017 Coordinator: CENER... Partners: HERA, CNTA Total budget: 0,4 M. Budget Cener: 0,32 M.

20 4. SYN2OL SYN2OL Funded by Navarre Government, Coordinator: CENER... Partners: HERA, CNTA Total budget: 0,4 M. Budget Cener: 0,32 M. Project objetives: Development of a fermentation process from syngas to produce ethanol and acetic acid Laboratory scale fermenters CENER s main tasks. Laboratory scale syngas fermentation tests (flasks / bottle ml) Bioreactor modification to improve mass transfer (3-5 L): syngas injection system and agitation impellers modification Laboratory scale syngas fermentation tests (bioreactor 3-5 L) Pilot scale syngas fermentation tests (40 L)

4. OPTIMIZATION AND UPSCALLING OF A FERMENTATION PROCESS OPTIMIZATION AND UPSCALLING OF A FERMENTATION PROCESS (CONFIDENTIAL) Private customer: 2016-2018 Project objetive: Optimization and scaling up

21 4. OPTIMIZATION AND UPSCALLING OF A FERMENTATION PROCESS OPTIMIZATION AND UPSCALLING OF A FERMENTATION PROCESS (CONFIDENTIAL) Private customer: Project objetive: Optimization and scaling up of a fermentation process for agrobiological product production Screen control of the 1000 L bioreactor CENER s main tasks. Product chromatographic characterization protocol development Fermentation test for production process optimization and upscalling up from flask to 1000 litters Economic and sustainability assessment

4. BUTANexT BUTANexT: Next Generation Bio-butanol 100 l fermenters in the Biochemical Process Unit H2020 LCE-11-2014 : 2015-2018 http://butanext.eu/ Coordinator: GreenBiologics.

22 4. BUTANexT BUTANexT: Next Generation Bio-butanol 100 l fermenters in the Biochemical Process Unit H2020 LCE : Coordinator: GreenBiologics... Partners: Técnicas Reunidas, CENER, Zabala Innovation Consulting, Universidad de Castilla-La Mancha (España), Metgen (Finlandia), C-TECH Innovation Limited y E4tech (Reino Unido), y VITO - Flemish Institute for Technological Research y Greenovate! Europe (Bélgica). Total budget: 4,7 M. Budget Cener: 0,68 M. Project objetives: Development and validation of a process with lower economic cost, energy efficient and sustainable for the conversion of lignocellulosic feedstocks and waste into biobutanol. CENER s main tasks. CENER is the scientific coordinator and is mainly involved in the process integration and scale-up at pilot scale of pretreatment, enzymatic hydrolysis, fermentation and in situ product recovery

4. BioPEST BioPEST Funded by Navarre Government, 2015-2016 Coordinator: CENER... Partners: IdAB, SIPCAM Total budget: 0,200 M. Budget Cener: 0,200 M.

23 4. BioPEST BioPEST Funded by Navarre Government, Coordinator: CENER... Partners: IdAB, SIPCAM Total budget: 0,200 M. Budget Cener: 0,200 M. Project objetives: Design and production a "on-demand" bioinsecticide effective for the control of the lepidopteran complex that cause pests in vine growing General view for the Biochemical process unit CENER s main tasks. Optimization of the production process of proteins / toxins and enhancers in fermenters Development of the industrial fermentation process with B. thuringiensis at scale of 1000 L

24 4. UPSCALLING OF A FERMENTATION PROCESS UPSCALLING OF A FERMENTATION PROCESS for PHB production (CONFIDENTIAL) Private customer: 2017 Project objetive: Scaling up of a fermentation process of conversion of cereal residue into Polyhydroxybutyrate (PHB), including: enzymatic hydrolysis, decanter, fermentation and centrifugation Detail of one of the fermenters in the Biochemical Process Unit CENER s main tasks. Project coordination: including equipment from vendors (decanter, centrifuge) Plant operation 1 st stage upscalling to 100 L 2 nd stage upscalling to 3000 L

$conversion of organic fraction of MSW into 2,3 BD, including: pretreatment, enzymatic hydrolysis, decanter, fermentation and centrifugation Detail of one of the fermenters in the Biochemical Process$

25 4. UPSCALLING OF A FERMENTATION PROCESS UPSCALLING OF A FERMENTATION PROCESS for 2,3 BD production (CONFIDENTIAL) Private customer: 2017 Project objetive: Scaling up of a fermentation process of conversion of organic fraction of MSW into 2,3 BD, including: pretreatment, enzymatic hydrolysis, decanter, fermentation and centrifugation Detail of one of the fermenters in the Biochemical Process Unit CENER s main tasks. Project coordination: including equipment from vendors (decanter, centrifuge) Plant operation 1 st stage upscalling to 100 L 2 nd stage upscalling to 3000 L

26 4. Examples of R&D projects: new projects PERCAL ( ) Project Title Chemical building blocks from versatile MSW biorefinery Program: H2020-BBI-PPP-2016 BIO BASED INDUSTRIES JOINT UNDERTAKING BRISK II ( ) Project Title Biofuels Research Infrastructure for Sharing Knowledge II Program: H2020-INFRAIA Integrating and opening research infrastructures of European interest AMBITION ( ) Project Title Advanced biofuel production with energy system integration Program:H2020: LCE European Common Research and Innovation Agendas (ECRIAs) in support of the implementation of the SET Action Plan BIORESCUE ( ) Project Title: Enhanced bioconversion of agricultural residues through cascading use Program: H2020-BBI-PPP BIO BASED INDUSTRIES JOINT UNDERTAKING CYCLALG ( ) Project Title: A Network of Technological Centres for the Development of a Microalgae Based Biorefinery Programa: Interreg POCTEFA.

27 4. Examples of R&D projects: new upscalling projects KL VAINILLINA ( ) Project Title: Valorización de lignina kraft para la producción de vainillina Program: Gobierno de Navarra BIOVALORIZACION ( ) Project Title: Valorización de la fracciones liquida y sólida de procesos de producción de biocombustibles de 2ª generación Program:. Gobierno de Navarra

28 ACKNOWLEDGEMENTS Contact: Dr.Javier Gil The construction of the CB2G has been funded by the Government of Navarre (Navarre Plan 2012), the Spanish Ministry of Science and Innovation (National Program for Scientific- Technological Infrastructures) and the European Fund for Regional Development (FEDER)

CASTILLA-LA MANCHA BIO-ECONOMY REGION PROJECT

CASTILLA-LA MANCHA BIO-ECONOMY REGION PROJECT Europe Challenge: Demonstration scale Lab Scale Demonstration Scale Biorefinery Mature Biorefinery Source: Bio-Based Industries Consortium CLaMber Plant Target