Protein Electrophoresis

Transcription

1 Protei Electrophoresis

2 Select the Best for Your Applicatio Orderig Iformatio PAGEr Gold Precast Gels 10 gels per bo Cocetratio Size 2D-well+ 8+1-well* 10-well 12-well 16-well 17-well* 4-12% 9 10 cm cm % 9 10 cm cm % 9 10 cm cm % 9 10 cm cm % 9 10 cm cm % 9 10 cm cm % 9 10 cm cm % 9 10 cm cm PAGEr Gold Scoutig Kit cm Select 6 gels of ay type cm Select 6 gels of ay type Specificatios Cassette Dimesios Cassette Thickess Gel Dimesios 9 cm 10 cm (L W) 0.49 cm (L W) 0.1 cm 10 cm 10 cm (L W) 0.55 cm (L W) 0.1 cm Gel Matri/Buffer: Polyacrylamide/Tris-Glycie Note: Gels do ot cotai SDS. Add SDS to sample buffer to create deaturig ruig coditios. Stackig Gel: 4% stackig gel Well Format: 2D well, 8+1 well*, 10 well, 12 well, 16 well, 17 well* * multichael pipette compatible well formats Cassette: Plastic Storage/Shelf Life: 2 C 8 C for 3 moths from date of maufacture Guarateed 10 weeks upo receipt Chamber Compatibility Maufacturer & Model Gel Size 9 10 cm cm Loza PAGEr Miigel Chamber FisherBiotech Fisher FB-VE 10-1 Fisher FB-VE 12-1 Fisher EC Ivitroge/Nove XCell SureLock MiiCell XCell I & II Bio-Rad Mii-PROTEAN I, II & 3 Ready Gel Cell Hoefer Mighty Small SE 250 SE 260 SE 280 Biometra Mii V Sigma Aldrich Mii Techware CBS Scietific MGV System Owl Separatio Systems Pegui P8DS-1 Daiichi/ISS 2 & 6 System Zais System 200 Some chambers may require adjustmet for optimal fit with PAGEr Gels. Cotact Techical Service for details. Separatio Rage i PAGEr Tris-Glycie Gels Polyacrylamide Size Separatio Cocetratio Rage 7.5% 50 kda-200 kda 10% 25 kda-200 kda 12% 20 kda-200 kda 15% 10 kda-50 kda 4 20% 5 kda-200 kda 10 20% 5 kda-150 kda 4 12% 25 kda-250 kda 8 16% 15 kda-200 kda

3 Precast Gels PAGEr Precast Gels Sharp, reliable, easy-to-use Protei Separatio with a PAGEr Precast Gel PAGEr Gels are easy-to-use protei miigels that offer sharper resolutio, more cosistet protei trasfer, ad loger shelf life tha ay other Tris-Glycie gel. Choose from multiple optios for deatured ad ative protei separatio over a wide molecular weight rage, i a array of cofiguratios i both 9 cm 10 cm ad 10 cm 10 cm sizes to fit popular chambers. Razor sharp resolutio Crisp separatio of proteis 5 kda 250 kda Easy-to-use Marked sample laes for easy loadig ad simple twist ope desig Versatile Multiple well formats ad gel cocetratios i 2 sizes to fit most chambers Tris-Glycie buffer Traditioal Laemmli separatio Fresh We ship fresh gels every time for guarateed performace Separatios o a 4 20% PAGEr Precast Gel. ProSieve Protei Markers laes 1, 3, 5, 7, 9, ad 11. E. coli etract i laes 2, 4, 6, 8, 10 ad 12. Proteis detected by Coomassie Brilliat blue stai. Bio-Rad Ready Gel PAGEr Precast Gel Resolutio compariso of 4 20 % Coomassie staied gels. Samples iclude a repeatig patter of ProSieve Protei Marker, ProSieve Color Protei Marker ad E. coli lysates. Comb Optio ad Well Volumes 2D well 550 µl sample, or 7 cm IPG strip, 12 µl marker 8+1 well* 30 µl sample 12 µl marker 10 well 32 µl 12 well 20 µl 16 well 14 µl 17 well 14 µl * 8+1-well ad 17-well gels are multichael pipette compatible

4 Chambers ad Protei Markers PAGEr Miigel Chamber Sharp performace ad simplicity The PAGEr Miigel Chamber is the easiest chamber you will ever use. The simple, lock-i-place core desig assures a tight, flat fit. No eed to remove the core, simply isert gels, close the clamps, fill with buffer ad ru. Rus oe or two PAGEr Gels ad accommodates a tak blottig module. Easy-to-use core elimiates leakig ad miimizes hadlig Perfect fit with 9 cm 10 cm ad 10 cm 10 cm PAGEr Gels Eve electrical force esures straight laes kda ProSieve Color Protei Marker kda PAGEr Gel Miigel Chamber Performace ProSieve Color Protei Markers ad E. coli lysate ru o a PAGEr Gel at 200V for 60 miutes i the PAGEr Miigel Chamber. ProSieve Protei Marker kda Markers ru o a 4 20% PAGEr Gel. The PAGEr Blot Module works directly i the PAGEr Miigel Chamber ad provides eceptioal blottig with a fast, simple protocol. Color-coded cassettes esure proper orietatio of the gel durig trasfer Trasfer time 90 miutes or less Higed cassette desig for easy assembly The system ca be purchased as a kit, icludig the PAGEr Miigel Chamber, PAGEr Blot Module ad spoge pads, or compoets may be purchased separately. ProSieve Color Protei Markers Sharp performace ad simplicity ProSieve Color Protei Markers are a easy method for moitorig protei separatio prior to staiig, ad provide accurate cofirmatio of protei trasfer i Wester blottig. Coveiet just add water ad load Sharp multi-colored, readily idetifiable bad patter ProSieve Protei Markers Sharp, accurate sizig of proteis ProSieve Protei Markers are desiged for accurate sizig of proteis 5 kda to 225 kda. The 50 kda bad is double-itesity for easy idetificatio. Orderig Iformatio PAGEr Miigel Chamber PAGEr Tak Blot Module PAGEr Miigel Chamber ad Blot Module Kit* Simple wide distributio of eact masses simplifies size determiatio Accurate recombiat proteis do ot cotai oligosaccharides that ca cause aomalous migratio, heterogeeous fuzzy bads, ad iaccurate size estimatio Orderig Iformatio ProSieve Color Protei Markers µl µl ProSieve Protei Markers µl * Icludes chamber, blottig cassette, ad spoge pads. (8/pack). Cotact Techical Service for iformatio about replacemets parts.

5 Stais ad Buffers SYPRO Protei Gel Stais Fast, sesitive, easy-to-use fluorescet gel stais SYPRO Ruby Stai SYPRO Red Stai Diluted i PBS (Polaroid photo UV light) SYPRO Tagerie Stai Diluted i PBS (Polaroid photo UV light) Coomassie Brilliat Blue Stai Serial dilutios of Loza s ProSieve Protei Marker 50 kda bad o 12% PAGEr Gold Precast Gels. Protei level idicated i aograms. SYPRO Stais are equisitely sesitive ad simple fluorescet protei gel stais that rival the sesitivity of the best silver stais for a rage of applicatios from 2D gel staiig to staiig gels prior to Wester blottig. SYPRO Ruby Stai is the ideal stai for 2D aalysis, offerig a liear quatitatio rage of over three orders of magitude SYPRO Red Stai is the fastest, easiest stai for routie protei detectio, requirig o fiatio or destai SYPRO Tagerie Stai does ot iterfere with protei activity ad is ideal for staiig gels prior to Wester trasfer SYPRO Ruby Blot Stai offers sesitivity levels that rival colloidal stais for detectig proteis o blots Orderig Iformatio SYPRO Ruby Protei Gel Stai L L ml SYPRO Ruby Protei Blot Stai ml SYPRO Tagerie Protei Gel Stai µl SYPRO Red Protei Gel Stai µl µl SYPRO Protei Gel Stai Photographic Filter square Select the Best Stai for your Applicatio Applicatio SYPRO SYPRO SYPRO Ruby Tagerie Red High performace staiig Staiig prior to Wester blottig 2D Electrophoresis Edma microsequecig Mass spectrometry Quatitatio Zymography Electroelutio Membrae staiig Protei epressio Detectio prior to Immuostaiig Difficult to stai proteis IEF Gels AccuGENE Electrophoresis Buffers Optimized performace from a closed reaget system AccuGENE Buffers are prepared with high quality reagets ad formulated to match PAGEr Precast Gels. These buffers use 18 megohm water. Products are filtered usig a 0.2-micro filter. Reliable maufactured accordig to strict quality cotrol stadards to esure lot-to-lot cosistecy Fleible customized solutios are available to meet idividual eeds Orderig Iformatio AccuGENE 10X Tris-Glycie Buffer 0.25 M Tris base, 1.92 M Glycie L L AccuGENE 10X Tris-Glycie SDS Buffer 0.25 M Tris base, 1.92 M Glycie 1% SDS L L

6 Cotact Iformatio: North America Customer Service: Techical Service: Outside USA: Olie Orderig Europe Customer Service: 32 (0) Techical Service: 32 (0) (Germa Speakig) 32 (0) (Frech Speakig) or Olie Orderig Iteratioal Cotact your local Loza Distributor Customer Service: , et Fa: Iteratioal Offices Australia Austria Belgium 32 (0) Demark Frace Germay Idia Italy The Netherlads Spai Switzerlad Uited Kigdom For Research Use Oly. Not for use i diagostic procedures. SYPRO is a trademark of Molecular Probes, Ic. Bio-Rad, MiiProtea ad ReadyGel are trademarks of Bio-Rad Laboratories, Ic. Pegui is a trademark of Owl Separatio Systems. NOVEX ad XCell SureLock are trademarks of Ivitroge, Ic. Coomassie is a trademark of Imperial Chemicals Idustries PLC. Biometra is a trademark of Biometra Biomediziische Aalytik GmbH. FisherBiotech is a trademark of Fisher Scietific Compay. Hoefer is a trademark of Hoefer Scietific Istrumets. All other trademarks herei are marks of the Loza Group or its affiliates Loza Rocklad, Ic. All rights reserved. P