Personal information. Giuseppina Barutello. Education and Training. Work experience. Name. Address. Via Torino 123, 10070, Cafasse (TO) Work adress

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1 Personal information Name Address Via Torino 123, 10070, Cafasse (TO) Work adress University of Turin, Department of Molecular Biotechnology and Health Sciences c/o Molecular Biotechnology Center, Via Nizza 52, 10126, Torino, Italy Telephone(s) Mobile: Fax(es) Nationality Date and place of birth Gender Italian 03/10/1985, Turin, Italy Female Education and Training January 2012 October 7 th 2011 April 15 th 2008 April 10 th 2008 Selected for the PhD program in Molecular Medicine XXVII cycle, University of Turin, Turin Master s Degree in Molecular Biotechnology discussing the experimental thesis A vaccine targeting Angiomotin alters tumor vessel permeability and hampers the growth of established tumors at University of Turin, Molecular Biotechnology Center, Turin. Evaluation 110/110 cum laude Degree in Biomedical Laboratory Tecniques discussing the experimental thesis Preclinical testing of a chimeric vaccine human / rat for the prevention of mammary carcinogenesis, Evaluation 107/110 State examination to obtain the license to practice the Laboratory technician profession July 2004 High school diploma Liceo delle Scienze Sociali F. Albert, Lanzo T.se, Italy. Evaluation 85/100 Work experience October January 2015 Position held Fellowship at the Department of Oncology-Pathology, Karolinska Institutet (Cancer Centrum), Stockholm, Sweden. Project Generation and characterization of new mouse models for the study of tumor angiogenesis. Supervisor: Prof. Lars Holmgren PhD Student in Molecular Medicine (XXVII cycle) at Laboratory of Immunology, Molecular Biotecnology Center, Torino. Supervisor: Prof. F. Cavallo Cooperation and continuous fixed-term contract with the European Consortium for Anticancer Antibody Development (EUCAAD), to carry out the job of laboratory technician in the biomedical research project assigned to the Department of Clinical and Biological Sciences of the University of Turin. Page 1/5 - Curriculum vitae of

2 International Foundation for Experimental Research Scholarship at Department of Clinical and Biology Sciences (Prof. Guido Forni), Laboratory of Immunology, University of Turin, Turin. Scholar Stage: Laboratory of Immunology, Department of Clinical and Biological Sciences, University of Turin c/o Molecular Biotechnology Center, Turin Scholar Stage: Service of Microbiology, Clinical Chemistry, Hematology and Pharmacy at the hospital Ciriè, Turin. Scholar Stage: Service of Pathological Anatomy at the Giovanni Bosco Hospital, Turin Principal occupational skills Cell Biology: cell cultures of tumor cell lines, Mixed lymphocytes cultures (MLC), Mixed lymphocytes and tumor cells cultures (MLTI), purification of lymphocyte populations, co-culture with transwell systems, in vivo cytotoxicity (CFDASE Cell Tracer Kit), proliferation assays, immunofluorescence techniques to reveal membrane and/or cytoplasmatic expression of molecole and cytokines (FACS), ELISA and ELISPOT, staining of histological and cytological samples; immunohistochemistry. Molecular Biology: amplification of DNA by PCR, extraction of DNA, RNA and proteins from cells and tissues, electrophoresis of DNA and RNA, RT-PCR for RNA analysis, transformation of competent bacteria, plasmid preparation and purification in large scale, Western blot, immunoprecipitation. Animal Biology: breeding transgenic and knockout mice, sampling and screening samples for genotype analysis, genomic DNA extraction for PCR, intraperitoneal, subcutaneous and intravenous injection, removal of organs, preparing samples for histology, in vivo transfection by electroporation of DNA plasmid, transplantation of tumor cells, inclusion and freezing of organs and tissues; cutting microtome cryostat. Teaching a.a and : Didactic collaboration at the University of Cuneo, Degree in Nursing, Immunology course Personal skills and competences Mother tongue Other language Technical skills and competences Driving licence Italian English Knowledge of basic computer programs (Word, Excel, Power Point) and graphic biostatistics program (GraphPad Prism 5.0). Laboratory instruments: Optical microscope, Confocal microscope, Microtome, Cryostat, Thermal cycler, Spectrophotometer (plate reader, NanoDrop), Flow Cytometer. Licence B Page 2/5 - Curriculum vitae of

3 Attended meetings and courses November 2008 December 2010 September 2011 June 2012 International Workshop Vaccines in the management of cancer: Where we are heading, Torino, Italy International Workshop International Workshop on metastasis research: unravelling cancer cell invasion and metastasis, Torino, Italy First Ascoli Piceno Conference on Gene Vaccination in Cancer, Ascoli Piceno, Italy (Poster presentation) StratCan Interactive Summer School in Tumor Immunology, Stockholm, Sweden September 2012 August 2013 October 2013 May th International Cancer Vaccine Symposium, Convitto della Calza, Firenze, Italy (Poster presentation) first evidence.-15th International Congress of Immunology, Milano, Italy (Poster presentation) first evidence - Second Gene Vaccination in Cancer, Ascoli Piceno (Poster presentation, awarded and selected for oral presentation) first evidence.- CIMT 2014, Mainz, Germany (Poster presentation) first evidence - IX National Conference of SIICA, Firenze, Italy (Poster presentation) September 2014 November 2014 Awards Multiplexing Elispot Workshop, Verona, Italy Tumor Microenvironment Course, Karolinska Institutet, Stockholm, Sweden. 1) March 27 th 2015: Premio CUS Torino Just the Woman I am-2015 for the best PhD research program. 2) October 11 th 2011: Second Gene Vaccination in Cancer Award (2 prize); Ascoli Piceno, Italy. Title of the poster awarded for the oral presentation: Maternal immunization: transfer of a protective anti-tumor immunity from mothers to the offspring; a first evidence. Publications 1) BARUTELLO G., Curcio C, Spadaro M, Arigoni M, Trovato R, Bolli E, Zheng Y, Ria F, Quaglino E, Iezzi M, Riccardo F, Holmgren L, Forni G, Cavallo F (2015). Anti-tumor immunization of mothers delays tumor development in cancer prone offspring. Oncoimmunology (IN PRESS) 2) Conti L, Ruiu R, BARUTELLO G., Macagno M, Bandini S, Cavallo F, Lanzardo S (2014). Microenvironment, Oncoantigens, and Antitumor Vaccination: Lessons Learned from BALBneuT Mice. BIOMED RESEARCH INTERNATIONAL, vol. 2014; p. 1-16, ISSN: , doi: /2014/ ) Olgasi C, Dentelli P, Rosso A, Iavello A, Togliatto G, Toto V, Liberatore M, BARUTELLO G., Musiani P, Cavallo F, Brizzi MF. (2014). DNA vaccination against membrane-bound Kit ligand: A new approach to inhibiting tumour growth and angiogenesis. EUROPEAN JOURNAL OF CANCER, vol. 50; p , ISSN: Page 3/5 - Curriculum vitae of

4 4) Di Gregorio E, Gianolio E, Stefania R, BARUTELLO G., Digilio G, Aime (2013). On the Fate of MRI Gd-Based Contrast Agents in Cells. Evidence for Extensive Degradation of Linear Complexes upon Endosomal Internalization. ANALYTICAL CHEMISTRY, vol. 85; p , ISSN: , doi: /ac400973q 5) Bandini S, Curcio C, Macagno M, Quaglino E, Arigoni M, Lanzardo S, Hysi A, BARUTELLO G., Consolino L, Longo DL, Musiani P, Forni G, Iezzi M, Cavallo F (2013). Early onset and enhanced growth of autochthonous mammary carcinomas in C3- deficient Her2/neu transgenic mice. ONCOIMMUNOLOGY, vol. 2; p. 1-14, ISSN: ) Arigoni M, BARUTELLO G., Riccardo F, Ercole E, Cantarella D, Orso F, Conti L, Lanzardo S, Taverna D, Merighi I, Calogero RA, Cavallo F, Quaglino E (2013). mir- 135b coordinates progression of ErbB2-driven mammary carcinomas through suppression of MID1 and MTCH2. AMERICAN JOURNAL OF PATHOLOGY; p , ISSN: ) Arigoni M, BARUTELLO G., Lanzardo S, Longo D, Aime S, Curcio C, Iezzi M, Zheng Y, Barkefors I, Holmgren L, Cavallo F. (2012). A vaccine targeting angiomotin induces an antibody response which alters tumor vessel permeability and hampers the growth of established tumors. ANGIOGENESIS, vol. 15; p , ISSN: In accordance with Legislative Decree 196/03 I authorize the treatment of my personal data. Date: 23/04/2015 Signature: Page 4/5 - Curriculum vitae of

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