Product Datasheet. Vinculin Antibody (hvin-1) NB Unit Size: 0.1 ml
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1 Product Datasheet Vinculin Antibody (hvin-1) NB Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 10/14/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at
2 NB Vinculin Antibody (hvin-1) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Target Molecular Weight 0.1 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal hvin-1 15mM Sodium Azide IgG1 Unpurified Ascites 116 kda Page 1 of 5 v.20.1 Updated 10/14/2018 Product Description Host Mouse Gene ID 7414 Gene Symbol Species Reactivity Notes Marker Specificity/Sensitivity Immunogen Product Application Details Applications VCL Human, Mouse, Rat, Amphibian, Bovine, Canine, Chicken, Turkey Frog (100%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Focal Adhesion Marker Specifically labels vinculin at cell-cell and cell-substrate contacts. Shows crossreactivity with smooth muscle metavinculin. Purified human vinculin from uterus. Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunocytochemistry Recommended Dilutions Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:400, Immunohistochemistry- Frozen 1:10-1:500, Immunocytochemistry
3 Images [NB ] - Mouse primary keratinocytes stained with the clone hvin-1. Image is courtesy of customer review. Page 2 of 5 v.20.1 Updated 10/14/2018 [NB ] - MCF7 breast carcinoma cells expressing GFP fused CIN85 were labeled at a 1:400 dilution. [NB ] - Primary human osteoblast cells labeled (red), actin was stained with Phalloidin (green) and nuclei stained blue. [NB ] - Double labeling of cultured chicken fibroblasts for vinculin using Monoclonal Anti-Human Vinculin and Rabbit Anti-Tubulin (green).
4 [NB ] - Using confocal microscopy, human skin fibroblasts. The actin filaments were labeled with phalloidin (red) and hyaluronan was stained with fluorescently labeled hyaluronan binding protein (blue). Green color indicates focal contacts. Page 3 of 5 v.20.1 Updated 10/14/2018 [NB ] - A computer-generated micrograph showing the superimposed immunofluorescent staining of chicken lens cells using FITC-phalloidin (green), Monoclonal Anti-Human Vinculin, clone No. hvin-1 (red) and Rabbit Anti-Pan Cadherin. [NB ] - Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hvin-1. Nuclei are stained with propidium iodide. Longitudinal section. All parts of the intercalated discs are stained. [NB ] - Human monocytes (J-111) labeled with Monoclonal Anti- Vinculin. Actin filaments (red) and nuclear DNA (blue) were stained with Phalloidin-TRITC and DAPI, respectively. Green-yellow color indicates focal contacts.
5 [NB ] - Primary human osteoblast cells labeled with Anti- Vinculin (red). From Eng San Thian, Materials Science and Metallurgy, University of Cambridge, Cambridge, United Kingdom. Page 4 of 5 v.20.1 Updated 10/14/2018 [NB ] - Staining of a formaldehyde-fixed section of human myocardium using Monoclonal Anti-Human Vinculin, Clone No. hvin-1. Nuclei are stained with propidium iodide. Transverse section. The sarcolemma of all cardiomyocytes are distinctly labeled. Publications da Rocha JT, Trapani L, Segatto M et al. Molecular Effects of Diphenyl Diselenide on Cholesterol and Glucose Cell Metabolism. Curr Med Chem [PMID: ] Chianale, F et al. Diacylglycerol Kinase-alpha Mediates HGF-induced Epithelial Cell Scatter by Regulating Rac Activation Membrane Ruffling. Mol. Biol. Cell /mbc.E Sep. [PMID: ]
6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NBL HAF007 NB720-B NBP Vinculin Overexpression Lysate (Native) Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG1 Isotype Control (MG1) Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:
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Product Datasheet rrna Antibody (Y10b) NB100-662 Unit Size: 0.1 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 16 Protocols, Publications, Related Products, Reviews, Research Tools and Images
More informationProduct Datasheet. CD31/PECAM-1 Antibody (JC/70A) NB ml. Unit Size: 0.1 ml
Product Datasheet CD31/PECAM-1 Antibody (JC/70A) NB600-562-0.1ml Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. After opening store under sterile
More informationProduct Datasheet. Collagen I alpha 1 Antibody (COL-1) NB ml. Unit Size: 0.02 ml
Product Datasheet Collagen I alpha 1 Antibody (COL-1) NB600-450-0.02ml Unit Size: 0.02 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications:
More informationProduct Datasheet. IgG Isotype Control NB mg. Unit Size: 10 mg. 2-8C / 2 years from date of receipt. Reviews: 6 Publications: 24
Product Datasheet IgG Isotype Control NB810-56910-10mg Unit Size: 10 mg 2-8C / 2 years from date of receipt Reviews: 6 Publications: 24 Protocols, Publications, Related Products, Reviews, Research Tools
More informationProduct Datasheet. Myosin Heavy Chain Antibody (3-48) NB Unit Size: 0.1 mg
Product Datasheet Myosin Heavy Chain Antibody (3-48) NB300-284 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 2 Publications: 8 Protocols,
More informationProduct Datasheet. Cytokeratin 1 Antibody (LHK1) NB Unit Size: 0.1 ml
Product Datasheet Cytokeratin 1 Antibody (LHK1) NB100-2756 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 10 Protocols, Publications,
More informationProduct Datasheet. 53BP1 [p Ser25] Antibody NB Unit Size: 0.1 ml. Store at 4C. Do not freeze. Publications: 11
Product Datasheet 53BP1 [p Ser25] Antibody NB100-1803 Unit Size: 0.1 ml Store at 4C. Do not freeze. Publications: 11 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb100-1803
More informationProduct Datasheet. Lightning-Link (R) APC-Cy5.5 Antibody Labeling Kit Unit Size: 1 Reaction (up to 1.5 mg) Store at -20C.
Product Datasheet Lightning-Link (R) APC-Cy5.5 Antibody Labeling Kit 764-0015 Unit Size: 1 Reaction (up to 1.5 mg) Store at -20C. Protocols, Publications, Related Products, Reviews, Research Tools and
More informationProduct Datasheet. Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] NB7539. Unit Size: 1 ml. Store at 4C. Do not freeze.
Product Datasheet Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] NB7539 Unit Size: 1 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 12 Protocols, Publications, Related Products, Reviews, Research
More informationProduct Datasheet. PINK1 Antibody BC SS. Unit Size: ml
Product Datasheet PINK1 Antibody BC100-494SS Unit Size: 0.025 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 10 Publications: 117 Protocols, Publications,
More informationProduct Datasheet. HIF-1 alpha Antibody NB Unit Size: 0.1 ml
Product Datasheet HIF-1 alpha Antibody NB100-134 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 13 Publications: 139 Protocols, Publications,
More informationProduct Datasheet. GAPDH Antibody (1D4) NB Unit Size: 0.1 ml
Product Datasheet GAPDH Antibody (1D4) NB300-221 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 17 Publications: 257 Protocols, Publications,
More informationProduct Datasheet. DRP1 Antibody NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Product Datasheet DRP1 Antibody NB110-55237 Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 14 Protocols, Publications, Related Products, Reviews, Research
More informationProduct Datasheet. AIF-1/Iba1 Antibody NB SS. Unit Size: 0.02 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 12 Publications: 70
Product Datasheet AIF-1/Iba1 Antibody NB100-1028SS Unit Size: 0.02 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 12 Publications: 70 Protocols, Publications, Related Products, Reviews, Research
More informationProduct Datasheet. F4/80 Antibody (CI-A3-1) NB Unit Size: mg
Product Datasheet F4/80 Antibody (CI-A3-1) NB600-404 Unit Size: 0.125 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 30 Protocols, Publications,
More informationProduct Datasheet. Ki-67/MKI67 Antibody (SP6) NB Unit Size: 0.5 ml. Store at 4C. Do not freeze. Reviews: 1 Publications: 29
Product Datasheet Ki-67/MKI67 Antibody (SP6) NB600-1252 Unit Size: 0.5 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 29 Protocols, Publications, Related Products, Reviews, Research Tools and
More informationProduct Datasheet. CD31/PECAM-1 Antibody NB Unit Size: 0.1 ml. Store at 4C. Do not freeze. Reviews: 4 Publications: 18
Product Datasheet CD31/PECAM-1 Antibody NB100-2284 Unit Size: 0.1 ml Store at 4C. Do not freeze. Reviews: 4 Publications: 18 Protocols, Publications, Related Products, Reviews, Research Tools and Images
More informationProduct Datasheet. CD63 Antibody (H5C6) NBP SS. Unit Size: ml
Product Datasheet CD63 Antibody (H5C6) NBP2-42225SS Unit Size: 0.025 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications: 13 Protocols, Publications,
More informationProduct Datasheet. IL-6 Antibody NB Unit Size: 0.2 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Product Datasheet IL-6 Antibody NB600-1131 Unit Size: 0.2 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 10 Protocols, Publications, Related Products, Reviews, Research Tools
More informationProduct Datasheet. CD31/PECAM-1 Antibody (MEC13.3) NB Unit Size: 0.1 ml
Product Datasheet CD31/PECAM-1 Antibody (MEC13.3) NB600-1475 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 2 Publications: 14 Protocols,
More informationProduct Datasheet. Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) NB Unit Size: 0.1 mg
Product Datasheet Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) NB500-169 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 6 Publications:
More informationProduct Datasheet. c-myc Antibody (9E10) NB Unit Size: 0.1 ml
Product Datasheet c-myc Antibody (9E10) NB600-302 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications: 26 Protocols, Publications,
More informationProduct Datasheet. GAPDH Antibody NB SS. Unit Size: ml. Store at 4C. Do not freeze. Reviews: 2 Publications: 13
Product Datasheet GAPDH Antibody NB300-322SS Unit Size: 0.025 ml Store at 4C. Do not freeze. Reviews: 2 Publications: 13 Protocols, Publications, Related Products, Reviews, Research Tools and Images at:
More informationProduct Datasheet. TLR7 Antibody NBP SS. Unit Size: mg
Product Datasheet TLR7 Antibody NBP2-24906SS Unit Size: 0.025 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications: 53 Protocols, Publications,
More informationProduct Datasheet. IL-1 beta/il-1f2 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 4 Publications: 15
Product Datasheet IL-1 beta/il-1f2 Antibody NB600-633 Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 4 Publications: 15 Protocols, Publications, Related Products, Reviews, Research
More informationProduct Datasheet. RPE65 Antibody (401.8B11.3D9) NB SS. Unit Size: 0.05 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Product Datasheet RPE65 Antibody (401.8B11.3D9) NB100-355SS Unit Size: 0.05 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 6 Publications: 43 Protocols, Publications, Related
More informationProduct Datasheet. LAMP-2/CD107b Antibody (H4B4) NBP SS. Unit Size: ml
Product Datasheet LAMP-2/CD107b Antibody (H4B4) NBP2-22217SS Unit Size: 0.025 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 2 Publications: 16 Protocols,
More informationProduct Datasheet. NK1R Antibody NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Product Datasheet NK1R Antibody NB300-101 Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 25 Protocols, Publications, Related Products, Reviews,
More informationProduct Datasheet. HIF-1 alpha Antibody (H1alpha67) NB Unit Size: 0.1 ml
Product Datasheet HIF-1 alpha Antibody (H1alpha67) NB100-123 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 7 Publications: 135 Protocols,
More informationProduct Datasheet. 53BP1 Antibody NB Unit Size: 0.1 ml. Store at -20 C. Reviews: 8 Publications: 309
Product Datasheet 53BP1 Antibody NB100-304 Unit Size: 0.1 ml Store at -20 C. Reviews: 8 Publications: 309 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb100-304
More informationProduct Datasheet. Nox4 Antibody NB SS. Unit Size: ml. Store at 4C. Do not freeze. Reviews: 7 Publications: 56
Product Datasheet Nox4 Antibody NB110-58849SS Unit Size: 0.025 ml Store at 4C. Do not freeze. Reviews: 7 Publications: 56 Protocols, Publications, Related Products, Reviews, Research Tools and Images at:
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