CASTILLA-LA MANCHA BIO-ECONOMY REGION PROJECT FUNDED BY:

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1 CASTILLA-LA MANCHA BIO-ECONOMY REGION PROJECT FUNDED BY:

2 The bioeconomy concept It is an economy that is based on ecological sensitive products and services produced by the use of biotechnology from renewable energy sources. Biorefinery Bioproducts Biofuel Food Ingredients Energy

3 Research & Development Statistics Europe lacks Demonstration Projects

4 Europe s R&D strategy Source: Bio-Based Industries Consortium

5 Europe Challenge: Demonstration scale Lab Scale Demonstration Scale Biorefinery Mature Biorefinery Europe is working in conducting demonstration scale projects

6 CLaMber Funding 4 M 16 M 20 M

7 CLaMber Project Target PLACING CASTILLA-LA MANCHA IN THE CENTER OF THE NEW EUROPEAN STRATEGY FOR BIOECONOMY Specific objectives: Create a technologically advanced biorefinery pilot plant that allows the research on production of innovative bioproducts. Create a technology hub in the region to enable synergies with both companies producing biomass and bioproducts users and encourage the creation and exploitation of new market niches. Revitalize the local economy and reverse the negative demographics that characterizes rural areas. Create a efficient public-private cooperation system in order to increase scientific and technological progress and to stimulate European and international research initiatives.

8 Why Castilla-La Mancha? High potential of agricultural and forestry residues, wastes from food and livestock industries. Huge quantities of different kinds of biomass all over the region. Castilla- La Mancha region has a very good availability to biomass. The agricultural sector represents a high percentage of the region GDP. % SECTOR GDP 6, ,1 13,8 AGRICULTURE INDUSTRY CONSTRUCTION SERVICES

9 Biomass availability in Castilla-La Mancha AGECAM WASTE BIOMASS AVAILABLE FOR ENERGY RECOVERY AGRICULTURAL FOREST WOOD INDUSTRY BIOMASS HERBACEOUS WOODY FOOD AND AGRICULTURAL INDUSTRY t/year Cereal straw Stem and cob corn Waste of sunflower fields Subtotal Vine pruning Olive pruning Fruit pruning Vine grubbing up Subtotal Firewoods, branches, etc Subtotal Sawmill ,50 Boards 3.524,60 Construction ,59 Containers 8.961,50 Others and bobber 2.628,80 Furniture ,20 Subtotal ,19 Brewers Grape pomace Olive pomace Subtotal TOTAL ,19

10 Biomass availability in Castilla-La Mancha PROBIOGAS, 2011 WASTE BIOMASS AVAILABLE FOR DIGESTION WASTE t/year WASTE t/year Pig manure Nonconforming fruits Cow manure Vegetable transformation Poultry manure Tuber transformation 379 Other species manure Citric transformation 0 Slaughterhouse raw material Fruit transformation Poultry Slaughterhouse raw material Brewer Housing raw material Alperujo 2Phases Meat and bone meal 0 Alpechín 3Phases WWTP sludge meat Vine raw material WWTP sludge dairy Cider raw material 0 Whey Sugar raw material Dairy raw material Cereal straw Fish raw material 27 Sludges IWWTP Vegetable Transf WWTP sludge fish 22 Energetic crops Surplus vegetables Glycerin Surplus citrics 0 Raw material DDGS (bioethanol) Surplus fruit 56 Raw material beet pulp (bioethanol) 0 Nonconforming vegetables Wholesale Nonconforming tubers Bars and Restaurants Nonconforming citrics 0 Hotels TOTAL: t/year

11 Change of raw material? BIOMASS CARBOHIDRATES LIGNINE FATS PROTEINS VITAMINS DYES FRESHENERS OTHER SMALL MOLECULES ORGANIC MATTER MINERALIZATION REACTIONS OF ANAEROBIC REDUCTION PRESSURE OXYGEN INDUSTRIAL USE HISTORY CENTURY XIX FOSSIL MATERIAL MIX OF HIDROCARBONS: ALKANES ALKINES ALKENES AROMATICS ALCOHOLS PHENOLS ETHERS ALDEHYDES Y KETONES CARBOXYLIC ACIDS OTHERS BIOMASS FOSSIL MATERIAL PRESENT

12 CLaMber Biomass BIOMASS LIGNOCELLULOSIC SUGARED/STARCHY Lignocellulosic crops Sugared crops OILY RESIDUAL MIX Manures Whey High loading WW (vinasse, alpechin, Grape Slaughterhouse marcs and wastes lees Alperujo Whey WWTP OFMSW Sludges Herbaceous agricultural Waste animal fats Woody Waste agricultural Manures vegetable wastes fatswastes Oily crops Starchy Sugared crops crops Lignocellulosic crops Oily crops etc.) Herbaceous agricultural Starchy crops OFMSW Grape marcs and lees WWTP Sludges Alperujo Waste vegetable fats wastes Woody agricultural wastes Waste animal fats High loading WW Slaughterhouse wastes (vinasse, alpechin), etc.)

13 CLaMber actions BIOMASS BIOTECHNOLOGY KNOWLEDGE CASTILLA-LA MANCHA FACILITIES Promotion of R&D activities on biotechnology developed by companies in Castilla La Mancha. Construction of a demonstration scale biorefinery for research in the production of high value added bioproducts and bio-fuels from biomass on a larger scale

14 First action involved in the CLaMber Project CONSTRUCTION OF A DEMONSTRATION SCALE BIOREFINERY INTEGRAL MODULAR FLEXIBLE INNOVATIVE 1 t dry material per day SERVICES: RENTAL OF FACILITIES PROCUREMENT OF R&D PROJECTS FOCUSING ON ASPECTS LIKE EFFICIENCY, COST REDUCTION AND VALIDATION OF NEW BIOCONVERSION PROCESSES PARTNER FOR COMPETITIVE FUNDINGS TRAINNING IN BIOTECHNOLOGY

15 Simplified flow diagram Wet biodegradable biomass (industrial, livestock and agricultural) Preparation and homogenization Acidogenic digestion Complete anaerobic digestion Volatile fatty acids Hydrogene Biofertilizers Digestate Biogas Animal Feed Oily wastes or crops (rape, alperujo, etc.) Pressing and extraction Oils Esterification Biodiesel Sugared and starchy wastes or crops (cereals, corn, beet, etc.) Trituration Extraction High added value extracts Glycerin Lignocelulosic wastes and crops (straw, reed, pruning, etc.) Trituration Raw pulp Extraction Raw Pulp Hydrolysis Lignocelulose Gasification Sugars Pretratement Syngas (CO+H2) Bioethanol Digestion Animal feed Celulose Hydrólysis Glucose Hemicelulose Hydrólysis Xylose Lignin Alcohol synthesis Bioalcohols (methanol) Fischer- Tropsch synthesis Biofuels Other reactions Other reactions Redox Phenols, cresols, vanillin, dimethylsulfide, dimethyl sulfoxide, etc. Levulinic acid, hydroxymetilfurfural, etc. Xylitol, acetic acid, furfural, etc. Acid-Base Biological Phenol, phenolic acids, catechol, etc. Vanillin, ferulic, cumaric and other acids, oxidized lignin for paints and coatings,etc. Thermal Ethylene, acetylene, acetic acid, phenol, methane, carbon monoxide, etc.

16 Biorefinery units UP-STREAM Objective: Conversion of biomass in a fermentable carbon source Reception and weighing of raw materials. Covered Storage of solids. Agitated, aerated and refrigerated storage of liquids. Concentration and/or clarification of liquids for their suitability as fermentation medium. Triturating, homogenization and screening (solids removal). Physical/chemical pretreatment to increase the capacity of hydrolysis (breakdown of lignocellulosic structure). Hydrolysis of sugar chains to monosaccharides. Hydrolysate conditioning by solid/liquid separation and concentration of the hydrolysate till its suitability as fermentation medium. Transference of conditioned products to the fermentation batching. Buffer storage capacity of different hydrolysates.

17 Biorefinery units MID-STREAM Objective: Fermenting or biotransform the carbon source generated in upstream into different products Microbiology laboratory for micro management (starters, inoculants, etc...) with capacity for a WCB. Propagation train for main fermentation. Main fermentation. Pilot or secondary unit for fermentation. Batch preparation unit of culture medium. Salts and nutrients store. On line sterilization unit of culture medium. Preparation, sterilization and dosage unit of feedings (including carbon source). Culture medium clarification/concentration. Centralized CIP unit for washing equipment.

18 Biorefinery units DOWN-STREAM Objective: Isolate the fermentation product of interest by interfaced biotechnology modules. Mechanical lysis. Chemical extraction with solvents. Mechanical extraction (press). Drying technology. Chromatography column. Enzymatic bio-catalytic reactions. Multipurpose reactor. Lyophilizer. Test box_1. Test box_2 (ATEX).

19 Biorefinery units UTILITIES Objective: Auxiliary services for the operation of the biorefinery units Electrical supply Steam Process water Cooling tower water Chiller water Glycol water Compressed air and process instrumentation Gases (NH 3, N 2, O 2 )

20 Second action involved in the CLaMber Project Issuance of a Pre-commercial Public Procurement (PPP) (8,6 Millions ) for conducting innovative R&D projects aimed at: SELECTION OF OPTIMAL RAW MATERIALS IMPROVEMENT OR DEVELOPMENT OF NEW BIOPROCESS Toma de muestra biogás V-2 V-1 RPMC TI Toma de muestra biogás V-5 V-4 DEVELOPMENT OF NEW BIOPRODUCTS RPMC TI Toma de muestra biogás V-8 V-7 RPMC TI SOCIOECONOMIC RESEARCH, NEW BUSINESS MODELS, LOGISTIC AND OTHER TECHNOLOGY CHALLENGES Colector Biogás C-2 Colector Biogás C-4 Colector Biogás C-6 Baño Criostatizado R-1 R-2 R-3 Baño Termostatizado V-3 Vaciado de reactor V-6 Vaciado de reactor V-9 Vaciado de reactor G-1 G-2 G-3 G-4 G-5 G-6 Alimentación Residuo A-1 Colector Residuo tratado C-1 Alimentación Residuo A-2 Colector Residuo tratado C-3 Alimentación Residuo A-3 Colector Residuo tratado C-5

21 Knowledge procurement ALIGNED WITH THE EUROPEAN R&D STRATEGY OF 2020 HORIZON PROGRAM, THE PUBLISHED TENDERS ARE RELATED TO THE SERVICE OF RESEARCH, DEVELOPMENT AND INNOVATION FOR THE COMPREHENSIVE UTILIZATION OF: LIGNOCELLULOSIC BIOMASS (CROPS AND WASTES). OILY BIOMASS (CROPS AND WASTES). AGRIFOOD RESIDUAL BIOMASS. LIVESTOCK RESIDUAL BIOMASS AND NON-AGRIFOOD BIOMASS.

22 Lignocellulosic biomass (crops and wastes) Optimization of the lignocellulosic material fractionation methods to achieve sustainable and economically viable processes. Improve the process parameters (conversion, yield...) compared to conventional production processes, achieving at least a conversion of 75% of lignocellulose into high value-added products. Getting bioproducts with lignocellulosic base whose production cost is no more than 2-3 times higher than those ones currently obtained. Obtaining bioproducts from lignin whose production cost is not more than 3-4 times than the energy value obtained from the lignin. Obtain at least 3 bioproducts that are competitive in the market from the economic, technological and environmental point of view against equivalent products. Getting bioproducts with at least 70% of its content obtained from bioprocesses, with emissions of less than 2.3 kg CO2eq / kg emission. Contribute to improve cooperation between suppliers and end users along the entire value chain of the process. Convert lignocellulosic biomass in a competitive feedstock for conversion to bioproducts. Revitalization of rural areas as well as forest and agricultural industries in the region of Castilla-La Mancha.

23 Oily biomass (crops and wastes) Develop and demonstrate new non-food oil crops, maximizing the use of currently underutilized agricultural resources for the production of bioproducts like biolubricants, biofuels and bioplastics. Transform the extracted oil from oil crops in biodegradable polymers, equivalent to the polyolefins obtained in the petrochemical industry. Preparation of bioproducts whose production cost is not greater than 3 times the cost of the currently obtained ones. Obtaining bioproducts with at least 70% of its content obtained from bioprocesses, with emissions of less than 2.3 kg CO2eq / kg. Contribute to strengthening the cooperation between suppliers and end users along the entire value chain of the process. Creating jobs for the development of entrepreneurial activities in the region of Castilla-La Mancha, both the primary sector, the secondary one (logistics, processing industry of bio...) and the tertiary one.

24 Agrifood residual biomass Demonstrate the existence of the entire value chain with high value-added bioproducts for new markets, produced from agrifood waste. Reduce the environmental impact of waste and decrease the production cost of bioproducts till the production cost of their commercial homologous products. Efficient waste valorization to improve the competitiveness of the industry where that waste is generated, contributing to rural development and job creation in rural areas. Demonstrate the later use of the waste from the valorization as a possible source of biodegradable and/or fermentable carbon for biofuels (hydrogen and biogas among others...). Demonstrate the recovery of biogas and other gases produced, especially for uses other than combustion.

25 Livestock residual biomass and non-agrifood biomass Improve the environmental performance and cost efficiency of bioproducts and bioprocesses. Decrease the carbon footprint by at least 20% compared to existing technologies. Technological validation of at least 2 new bioproducts. Technological certification of fertilizer with an improved nutrient concentration and a usability for many plants. Facilitate the replacement of at least 10% of the nitrogen and phosphorus from commercial fertilizers with recycled compounds. Contribute to strengthening cooperation between suppliers and end users along the entire value chain of the process. Convert the residual biomass described in a competitive feedstock for its conversion to bioproducts.

26 THANK YOU!!!