Product Datasheet. Ki-67/MKI67 Antibody (SP6) NB Unit Size: 0.5 ml. Store at 4C. Do not freeze. Reviews: 1 Publications: 29

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1 Product Datasheet Ki-67/MKI67 Antibody (SP6) NB Unit Size: 0.5 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 29 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 12/17/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Ki-67/MKI67 Antibody (SP6) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer 0.5 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Store at 4C. Do not freeze. Monoclonal SP6 0.05% Sodium Azide IgG Tissue culture supernatant PBS and BSA Page 1 of 5 v.20.1 Updated 12/17/2017 Product Description Host Rabbit Gene ID 4288 Gene Symbol Species Reactivity Notes Marker Specificity/Sensitivity Immunogen MKI67 Human, Mouse, Rat, Canine Mouse reactivity reported in scientific literature (PMID: ). Reactivity of Ki-67 antibody' clone SP6 with Canine/Dog species is reported in PMIDs , and Proliferation Marker Ki67 (SP6) A synthetic peptide from C-terminus of human Ki-67. Product Application Details Applications Recommended Dilutions Application Notes Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:200, Immunohistochemistry- Frozen 1:10-1:500, Immunocytochemistry 1:100 Suggested incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mm citrate buffer, ph 6.0 prior to immunostaining. Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID ) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID )

3 Images Immunohistochemistry: Ki-67/MKI67 Antibody (SP6) [NB ] - Paraffin-embedded alcohol fixed rat spleen tissue (20x). Antigen retrieval ph 9. Ki67 dilution 1:100 incubation ON 4C. This image was submitted via customer review. Page 2 of 5 v.20.1 Updated 12/17/2017 Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [NB ] - Formalin fixed paraffin embedded human tonsil stained with Ki- 67 antibody. Immunocytochemistry: Ki-67/MKI67 Antibody (SP6) [NB ] - Ki67 staining in mouse thyroid tissue at pre-tumor stage(green). Dilution is 1:100. This image was submitted via customer Review. Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody (SP6) [NB ] - Formalin fixed paraffin embedded human tonsil stained with Ki- 67 antibody

4 Publications Penna I, Gigoni A, Costa D et al. The inhibition of 45A ncrna expression reduces tumor formation, affecting tumor nodules compactness and metastatic potential in neuroblastoma cells. Oncotarget. Jan :00AM [PMID: ] (IHC, Mouse) Ueha R, Ueha S, Sakamoto T et al. Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice. Neurotox Res 2016 Aug [PMID: ] Migita H, Yoshitake S, Tange Y et al. Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats. Nephrourol Mon 2016 Jan [PMID: ] Ueha R, Ueha S, Kondo K et al. Damage to Olfactory Progenitor Cells Is Involved in Cigarette Smoke-Induced Olfactory Dysfunction in Mice. Am J Pathol 2016 Mar [PMID: ] Sumis A, Cook K, Andrade FO et al. Social isolation induces autophagy in the mouse mammary gland: link to increased mammary cancer risk. Endocr. Relat. Cancer. Aug :00AM [PMID: ] (IHC, Mouse) Kalliokoski S, Piqueras VO, Frias R et al. Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids. Aug :00AM [PMID: ] (IHC, Mouse) Rath SK, Deb M, Sengupta D et al. Silencing of ZRF1 impedes survival of estrogen receptor positive MCF-7 cells and potentiates the effect of curcumin Tumour Biol Jun 27 [PMID: ] (IHC, Human) Migita H, Yoshitake S, Tange Y. Hyperbaric Oxygen Therapy Suppresses Apoptosis and Promotes Renal Tubular Regeneration After Renal Ischemia/Reperfusion Injury in Rats Nephro-Urology Monthly. Jan :00AM (IHC- P, Rat) Vesci L, Carollo V, Roscilli G et al. [Corrigendum] Trastuzumab and docetaxel in a preclinical organotypic breast cancer model using tissue slices from mammary fat pad: Translational relevance. Oncol Rep 2016 Jan [PMID: ] Tukler Henriksson J, Coursey TG, Corry DB et al. IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells. Invest Ophthalmol Vis Sci 2015 Jul [PMID: ] (ICC/IF, Mouse) Falkenberg N, Hofig I, Rosemann M et al. Three-dimensional microtissues essentially contribute to preclinical validations of therapeutic targets in breast cancer. Cancer Med Jan 14 [PMID: ] (IHC-P, Human) Du Z, Bondarenko O, Wang D et al. Ex Vivo Assay of Electrical Stimulation to Rat Sciatic Nerves: Cell Behaviors and Growth Factor Expression. J. Cell. Physiol Oct 30 [PMID: ] (IHC-P, Rat) More publications at Page 3 of 5 v.20.1 Updated 12/17/2017

5 Procedures Immunohistochemistry-Paraffin Protocol Specific for NB : Ki67 Antibody (SP6) Materials Page 4 of 5 v.20.1 Updated 12/17/2017 1) 1 Phosphate buffered saline (ph 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/l 2) Citrate buffer, 0.01 M, ph6.0, Sodium Citrate 3g, Citric acid 0.4g 3) 3% Hydrogen peroxide 4) Primary antibody 5) Blocking serum (normal serum) 6) Biotinylated secondary antibody 7) DAB staining kit Methods 1. Dewax and hydration of slides using xylene and EtOH: Dry slides for 20 min in a 60 C oven Add Xylene, 2 x 10 min 100%, 95%, 80%, and 70% EtOH, 5 min each EtOH concentration Rinse in PBS, 5' 2 Antigen retrieval method (only for paraffin slides) 1a. High-pressure antigen retrieval procedure (recommended method) Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 ml of Citrate buffer (0.01M, ph6.0) within a pressure cooker Once steam is produced, and ONLY when steam is visible, from the pressure cooker (usually min), the required high-pressure will have been reached, and slides will be incubated for 2 min. Turn off heat, and allow buffer and slides to cool to room temperature Slides are then rinsed in PBS for 5 minutes 2. Add 3% hydrogen peroxide solution, 10'at RT, then PBS, 3X5' 3. Normal blocking serum, 20'at RT 4. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C 5. Rinse with PBS, 3 X 5' each rinse 6. Add Biotin-conjugated second antibody, 10'at RT 7. Rinse with PBS, 3 X 5' each rinse 8. Add Streptavidin-Peroxidase, 10'at RT 9. Rinse with PBS, 3 X 5' each rinse 10. Staining with DAB solution, 2-5'under microscope 11. Stop the reaction by washing in tap water 12. Counterstain in Haematoxylin for 3-5 minutes %, 80%, 95% and 100% ethanol, 5x2', xylene 2 x 10'

6 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB NB HAF008 NB7160 NBP Human Tonsil Whole Tissue Lysate (Adult Whole Normal) Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody [HRP] Rabbit IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: