Introduction to Biochemistry - BIOL 202 University Studies Program. Course Outline
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1 Intrductin t Bichemistry - BIOL 202 University Studies Prgram Curse Outline COURSE IMPLEMENTATION DATE: January 1984 OUTLINE EFFECTIVE DATE: January 2018 COURSE OUTLINE REVIEW DATE: September 2023 GENERAL COURSE DESCRIPTION: BIOL 202 is a cntinuatin f BIOL 201. Thrugh lectures and labs the curse emphasizes the structural and functinal aspects f cellular chemistry. Tpics include cellular energetics, enzyme kinetics, respiratin, phtsynthesis, membrane transprt, the genetic cde, glycbilgy, lipid bilgy, and prtein bilgy. The labratry exercises emphasize prper experimental techniques, data cllectin and analysis and technical writing skills. Prgram Infrmatin: This curse can be used as either a required curse r an elective in several University Studies Prgrams. It is intended primarily fr secnd-year university transfer students wishing t majr in bilgy r related fields. Delivery: This curse is delivered face t face. COTR Credits: 3 Hurs fr this curse: 90 hurs Typical Structure f Instructinal Hurs: Practicum Hurs (if applicable): Instructinal Activity Duratin Type f Practicum Duratin Lecture Hurs 45 On-the-jb Experience Seminars / Tutrials Frmal Wrk Experience Labratry / Studi Hurs 45 Other Practicum / Field Experience Hurs Ttal Other Cntact Hurs Ttal 90 Cllege f Rckies 1
2 Curse Outline Authr r Cntact: Lynnette Kuervers, BSc, PhD Signature APPROVAL SIGNATURES: Department Head Ildi Walkley walkley@ctr.bc.ca Dean f Business and University Studies Darrell Bethune bethune@ctr.bc.ca Department Head Signature Dean Signature EDCO Valid frm: January 2018 September 2023 Educatin Cuncil Apprval Date COURSE PREREQUISITES AND TRANSFER CREDIT Prerequisites: BIOL 201 r the equivalent r permissin f the instructr Crequisites: CHEM 202 Prir Learning Assessment (FA): Credit can be awarded fr this curse thrugh FA Yes N Learners may request frmal recgnitin fr flexible assessment at the Cllege f the Rckies thrugh ne r mre f the fllwing prcesses: External Evaluatin, Wrksite Assessment, Demnstratin, Standardized Test, Self-assessment, Interview, Prducts/Prtfli, Challenge Exam. Cntact an Educatin Advisr fr mre infrmatin. Transfer Credit: Fr transfer infrmatin within British Clumbia, Alberta and ther institutins, please visit Students shuld als cntact an academic advisr at the institutin where they want transfer credit. Prir Curse Number: Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 2
3 Textbks and Required Resurces: Textbk selectin varies by instructr and may change frm year t year. At the Curse Outline Effective Date the fllwing textbks were in use: Nelsn, D and Cx, M Lehninger: Principles f Bichemistry 7th. Ed. WH Freeman Please see the instructr s syllabus r check COTR s nline text calculatr fr a cmplete list f the currently required textbks. LEARNING OUTCOMES: Upn the successful cmpletin f this curse, students shuld be able t: Fundatins f Bichemistry Summarize the fundatins f bichemistry including, a brief cmparisn f eukarytic and prkarytic cells, the sterespecific interactins between mlecules, the cncept f entrpy and energy cupling reactins, and the evlutin f hereditary infrmatin Use the equilibrium cnstant, K eq, t calculate whether an enzymatic reactin is at equilibrium within a cell Water Describe the nncvalent interactins between water and majr cellular cmpnents Apply the van t Hff equatin fr smtic strength under varying cnditins r different rganelles Use the Hendersn-Hasselbalch equatin t calculate the acid dissciatin cnstant, pk a, and ph Describe the rle f a buffer in slutin Cmpare and cntrast the rle f water as a reactant with its rle as a slvent. Amin Acids, Peptide, and Prteins Recgnize the varius grupings f amin acids Describe the nncvalent interactins f amin acids within a prtein mlecule in a cellular envirnment Cmpare the current methds fr prtein islatin Define a cnsensus sequence Define biinfrmatics and use existing nline tls t analyze a DNA sequence, such as FASTA, BLAST, and Clustal Omega Summarize the fur levels f prtein structure Discuss the imprtance f amin acid sequences as well as nn-cvalent interactins in the prcess f prtein flding. Calculate the receptr-ligand dissciatin cnstant, K d f a prtein and ligand Enzymes Describe the different types f enzymes Describe the rle f binding energy in specificity and catalysis Derive the Michaelis-Menten equatin Calculate the Michaelis cnstant, K m, fr a given enzyme Observe and calculate K m in the lab fr a given enzyme with and withut an inhibitr present, and under varying cnditins Describe the effect f phsphrylatin n specific enzymatic reactins Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 3
4 Carbhydrates and Glycbilgy Recgnize and describe the structure f mnsaccharaides and disaccharides Cmpare and cntrast the main types f plysaccharides Define a glyccnjugate Define glycbilgy and describe the structure and functin f a glyccnjugate Cmpare current methds used in glycbilgy Nucletides and Nucleic Acids Define the cmpnents f a nucletide and nucleic acid Describe the structures f a DNA mlecule Describe the structures f an RNA mlecule Describe current methds used in DNA sequencing DNA-Based Infrmatin Technlgies Describe current technlgies used in genmics Lipids Cmpare and cntrast strage lipids, membrane lipids, and signaling lipids Describe current technlgies used in the study f lipids Bilgical Membranes and Transprt Describe the cmpnents and cncept f the fluid msaic mdel Cmpare and cntrast the different lipids within a cell membrane Cmpare and cntrast the different prteins assciated with the cell membrane Describe the dynamics f lipids within a bilayer Cmpare and cntrast the different mdes f transprt acrss a cell membrane Calculate the energy cst f pumping a given slute acrss a cell membrane List the measurements that are required t calculate the energetics f active transprt f a given mlecule Bienergetics and Metablism Calculate Gibbs free energy, Enthalpy and Entrpy f a given system Describe each f the fllwing reactin types and hw they may affect a chemical reactin within a living cell Reactins that make r break carbn-carbn bnds Internal rearrangements, ismerizatins, and eliminatins Free-radical reactins Grup transfers Oxidatin-reductins Define phsphrylatin ptential, G p Relate xidatin-reductin reactins t a bilgical system Calculate free-energy change f a redx reactin f a given bichemical reactin Glyclysis List the steps f the tw phases f glyclysis Identify the steps that require ATP, generate ATP and generate NADH Draw ne f the steps f glyclysis that generates ATP Citric Acid Cycle List the steps f the citric acid cycle List the enzymes f the pyruvate dehydrgenase cmplex Define substrate channeling using the frmatin f acetyl-ca as an example Identify the steps f the citric acid cycle that generate NADH and FADH 2 Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 4
5 Oxidative Phsphrylatin Identify the psitins f the mlecules invlved in xidative phsphrylatin n a mitchndrin Describe the flw f electrns and prtns during xidative phsphrylatin Describe the structure f ATP synthase Describe the rle f ATP synthase in the generatin f ATP Phtsynthesis Relate chlrplast structure t the capturing f energy frm a phtn Cmpare and cntrast the tw phtchemical reactin centers frm plants Describe the flw f electrns during phtsynthesis Describe the light-driven splitting f water Cmpare and cntrast the ATP synthase mlecule fund in chlrplasts with the ATP synthase mlecule fund in mitchndria List the mlecules and the main reactins f the three stages f carbn dixide assimilatin List the mlecules and main reactins f the bisynthesis f starch, sucrse, and cellulse Genes and Chrmsmes Describe the steps and enzymes invlved in the frmatin f a chrmsme List the elements cntained within a chrmsme List the mlecules that make up a chrmsme Calculate the superhelical density f a given DNA mlecule DNA Metablism Describe the steps f DNA replicatin Describe the rle f the DNA replicatin enzymes Describe the different methds f DNA repair and the enzymes invlved Describe the different methds f DNA recmbinatin RNA Metablism Describe the steps f RNA synthesis Describe the rle f the RNA synthesis enzymes Describe the DNA cmpnents, RNA cmpnents, and prtein cmpnents invlved in regulatin the synthesis f an RNA mlecule Cmpare and cntrast rrna, mrna, trna, snrnas, and mirnas Prtein Metablism Translate a genetic sequence int a peptide Describe the nncvalent interactins f the resulting peptide within a given slute Describe the steps f prtein synthesis Describe the rle f the prtein synthesis enzymes and their interactin with the nucleic acids List the methds used in a cell fr prtein targeting and degradatin This curse shuld help students: cmprehend and interpret detailed scientific and/r technical infrmatin frm text search fr infrmatin in the prfessinal literature critically evaluate infrmatin fr accuracy, relevance and imprtance think critically and act lgically t evaluate situatins apply prblem-slving skills assess and apply ptential mathematical strategies fr suitability and effectiveness wrk effectively with thers in a labratry situatin receive, cmprehend and interpret a sequence f instructins Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 5
6 plan and sequence a number f verlapping activities use equipment requiring careful prcedures draw reasnable cnclusins frm bservatins. COURSE TOPICS: 1. Fundatins f Bichemistry 2. Water, ph, and Buffers 3. Prtein Structure and Functin 4. Enzyme Kinetics 5. Carbhydrates and Glycbilgy 6. Nucleic Acids 7. Lipids and Cell Membranes 8. Bienergetics and Cellular Metablism 9. Cellular Respiratin 10. Phtsynthesis 11. Mlecular Genetics 12. Current Techniques in Bichemistry See instructr s syllabus fr the detailed utline f weekly readings, activities and assignments. EVALUATION AND ASSESSMENT Lecture Midterm(s)/Unit Exam(s) Curse Assignments Final Exam Lab Lab Reprts/Assignments Assignments Ttal % Of Ttal Grade 20% 15% 30% 35% 100% Please see the instructr s syllabus fr specific classrm plicies related t this curse, such as details f evaluatin, penalties fr late assignments, and use f electrnic aids. EXAM POLICY: Students must attend all required scheduled exams that make up a final grade at the appinted time and place. Individual instructrs may accmmdate fr illness r persnal crisis. Additinal accmmdatin will nt be made unless a written request is sent t and apprved by the apprpriate Department Head prir t the scheduled exam. Any student wh misses a scheduled exam withut apprval will be given a grade f 0 fr the exam. Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 6
7 COURSE GRADE: Curse grades are assigned as fllws: Grade A+ A A- B+ B B- C+ C C- D F Mark (Percent) < 50 A grade f "D" grants credit, but may nt be sufficient as a prerequisite fr sequential curses. ACADEMIC POLICIES: See fr general cllege plicies related t curse activities, including grade appeals, cheating and plagiarism. COURSE CHANGES: Infrmatin cntained in curse utlines is crrect at the time f publicatin. Cntent f the curses is revised n an nging basis t ensure relevance t changing educatinal, emplyment, and marketing needs. The instructr endeavurs t prvide ntice f changes t students as sn as pssible. The instructr reserves the right t add r delete material frm curses. Cllege f Rckies Dec. 13, 2017 sk Z:\OUTLINE\2018_Winter\BIOL202.dcx 7
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