Rose Midge Research Report 2006
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1 Rose Mige Reserh Report 26 Stuy Title: Evlution of hemil n iologil tretments for ontrol of rose mige (Dsineur rhoophg Coquillet): effiy n rop tolerne. Reserher: Dr. Jnie Elmhirst, Elmhirst Dignostis & Reserh, 727 Riversie Street, Aotsfor, BC, V4X 1T6. Tel: ; E-mil: Jnie.elmhirst@shw. Ojetives: 1) To ompre the effiy of hemil n iologil tretments for ontrol of rose mige. 2) To etermine whih tretments isply the lest phytotoxi effets n the est overll plnt qulity. 3) To otin effiy t on other pests of roses suh s phis n thrips, if present. Introution: Rose mige (Dsineur rhoophg Coquillet: Dipter: Ceiomyiie) is n inresing pest of roses worl-wie. Lrvl (mggot) feeing ours within emerging lef n flower us using newly-eveloping us to wither n turn lk. Infesttions of rose mige n use omplete filure of loom yle resulting in unmrketle plnts. Although mge n e oserve on outoor-grown roses in ostl British Columi from erly spring (lte Jnury) to lte Otoer, pek injury typilly ours in mi-june to erly July with seon pek in lte August/Septemer. There is n immeite nee for ontrol of this pest in ommeril nurseries n lnspe grens. This tril ompres the effiy of pretory nemtoes n mites s well s pyrethrois n metin for ontrol of this pest. AVID 1.9% EC (Syngent Crop Protetion In.) ontins metin whih is nturlly erive sustne proue y soil miroorgnism lle Streptomyes vermitilis. When pplie to folige, metin penetrtes plnt tissues n remins there so pests injest the hemil uring feeing. MATADOR 12 EC (Syngent Crop Protetion In.) ontins photostle, syntheti pyrethroi, lm-yhlothrin, n is registere in Cn for ontrol of pple lef urling mige n swee mige plus mny other inset pests of vegetle, erry, fruit n fiel rops. DOKTOR DOOM (Ultrsol Inustries Lt.) is rey-to-use (RTU) permethrin formultion tht n e pplie to roses vi soil or folir pplition. The iologil tretments inlue in this tril were Steinernem feltie pretory nemtoe (Beker Unerwoo) s oth soil n folir pplition, n n unientifie speies pretory mite (Applie Bionomis) pplie to the soil. These pretors fee on the lrve n pupe of the other Diptern speies suh s fungus gnts n thrips. Life Cyle: The life yle of the rose mige tkes pproximtely ys n strts with the emergene of the ults from the soil in the lte spring, whih fly to the emerging tips of rose nes. The femles then ly eggs in the sepls of the flower us or etween fole leves of the lefy tips. Eggs hth within two ys n mggots fee n mture within the us. Within five to seven ys, mture lrve rop to the soil to pupte y onstruting smll, white silken ooon from whih the ult emerges n re-strts the 1
2 life yle. Soil temperture is the min ftor ffeting pupl evelopment n ult emergene. Crop: Hyri rose (Ros L. x hyri) v. Ornge Blossom Speil Pests Trgete: Rose mige (Dsineur rhoophg Coquillet); phis; thrips Lotion: Lngley, British Columi, Cn Mterils: Avi 1.9% EC (metin, 19 g/l), Doktor Doom (.2% permethrin RTU), Doktor Doom (.% permethrin RTU), Steinernem feltie pretory nemtoe ( million per pkge); sp. pretory mite (2,/L), Mtor 12 EC (lmyhlothrin, 12 g/l). Methoology: The tril ws onute from June to Otoer, 26 t ommeril nursery in Lngley, British Columi, Cn on outoor, ontiner-grown roses v. Ornge Blossom Speil. Roses were proue from uttings roote in the greenhouse in Mrh n trnsplnte into stnr sn/rk/pet mix ph 6. in one-gllon pots whih were ple outoors on My 31 st. s were seprte into four replites in rnomize omplete lok (RCB) esign. Eh plot onsiste of four pots (surfe re.16 m 2 ) for totl re of.64 m 2 per tretment. Plnts were overhe irrigte, fertilize n prune one y the grower in lte July s per ommeril prtie. Suue MAXX (metlxyl-m) ws pplie t.8 ml/l on Aug. 29 to ontrol owny milew. To ensure inset pressure within the tril, mige-infeste us pike from neighouring plnts were ple in extr pots spe etween reps I n II n reps III n IV n reple every two weeks, strting June 2. Blooms n infeste us were remove eh week efore tretments were pplie. Ault mige emergene ws monitore weekly with two yellow stiky rs ple etween the four replites. Applition Metho: s were pplie to one-gllon ontiner plnts on 14 y intervl strting June n ening Sept. 26, with the exeption of the DOKTOR DOOM.% soil tretment whih ws pplie monthly on June 6, July 4, August 1 n Aug. 29. AVID, MATADOR n DOKTOR DOOM.2% were pplie s folir sprys in solution volume of 16 ml per 16 one-gllon pots (64 m 2 ), using CO 2 kpk spryer t 4 psi (276 kp) n single justle nozzle. AVID 1.9% EC (metin) ws pplie t onentrtion of.6 ml/l fter the solution ws juste with phosphori i to ph of.. MATADOR 12 EC (ph ) ws pplie t 83 ml/l n 14 ml/l. DOKTOR DOOM.2% rey-to-use (RTU) onentrtion ws pplie s fine mist 3-4 m ove the plnts n llowe to settle onto the folige. DOKTOR DOOM.% RTU ws pplie to the soil surfe of eh pot in totl pplition volume of 6 ml per pot in split pplition of 3 ml sprye onto the soil surfe n worke in lightly, followe y seon surfe spry of 3 ml one to two hours lter. One million pkge of pretory nemtoes (Steinernem feltie) ws ivie in hlf (2 million) n mixe in 1 L of wter; 2 ml (one up) per pot ws poure onto the soil 2
3 surfe n 2 ml per plnt ws sprye onto folige with hn-spry ottle. sp. pretory mites (2,/L) were pplie to the soil surfe t hlf tespoon per pot. Tle Timing n Prout Rte Applition Metho 1. (wter lone) Avi 1.9% EC 14 ys:.6 ml/l Folir spry; 1 ml solution per plnt (metin) 3. Doktor Doom 14 ys: RTU Folir mist; 1 ml sprye pproximtely 3 m (.2% permethrin) 4. Doktor Doom (.% permethrin). Steinernem feltie pretory nemtoe 6. sp. pretory mite 7. Mtor 12 EC (lm-yhlothrin) 8. Mtor 12 EC (lm-yhlothrin) Monthly: RTU 14 ys: ½ pk = 2 million/1 L wter 14 ys: 2,/L Soil: ½ tsp per pot ove s fine mist Soil: RTU; 3 ml per pot pplie to soil surfe n lightly worke in; 3 ml poure onto surfe gin within 1-2 hours = totl 6 ml/pot Folir + Soil: 2. million nemtoes/l wter; 2 ml per pot soil renh + 2 ml per plnt folir pplie in hn-spry ottle 14 ys: 83 ml/h Folir spry; 1 ml per plnt 14 ys: 14 ml/h Folir spry; 1 ml per plnt The numer of lef n flower us infeste or showing mige, thrips or phi mge ws reore weekly. The totl numer of us in eh plot ws ounte to lulte perentge of ffete us. Phytotoxiity (stunting, hlorosis, nerosis, lef istortion or u ortion) ws rte on sle of to 1, where = no visile symptoms n 1 = plnt eth. Sttistil nlysis (ANOVA) ws performe using CoStt Version 6.24, 23, CoHort Softwre, Monterey, Cliforni, USA, Copyright Results: Tle 1: Men sum of us infeste/mge y rose mige per tretment per plot. 1 Men Infeste Men Lef n Flower % Infeste % Infeste Bus 3 Men % Mige Dmge Reution wrt Avi 1.9% EC (.6 ml/l) 3. Doktor Doom % (Folir) 4. Doktor Doom % (Soil). Steinernem feltie 6. sp
4 7. Mtor 12 EC (83 ml/h) 8. Mtor 12 EC (14 ml/h) Men of 4 replites per tretment, RCB esign, 4 plnts per plot. 2 Numers followe y the sme letter re not signifintly ifferent in LSD t P<.. 3 Numers followe y the sme letter re not signifintly ifferent in Dunn s MR Test t P<.. Tle 2: Men sum of us infeste with thrips n shoots with phis per tretment per plot n % reution ompre to the untrete hek. 1 Men No. Thrips-Infeste % Thrips Reution wrt 2 Men Aphi- Infeste Shoots 3 % Aphi Reution wrt Avi 1.9% EC (.6 ml/l) Doktor Doom.2% (Folir) Doktor Doom.% (Soil) Steinernem feltie sp Mtor 12 EC (83 ml/h) Mtor 12 EC (14 ml/h) Men of 4 replites per tretment, RCB esign, 4 plnts per plot. 2 Numers followe y the sme letter re not signifintly ifferent in LSD t P<.. 3 Numers followe y the sme letter re not signifintly ifferent in Dunn s MR Test t P<.. Figure 1: Men sum of us infeste/mge y rose mige per tretment. 1 % Infeste us Avi Steinernem Mtor 83mL Mtor 14mL 1 Numers followe y the sme letter re not signifintly ifferent in LSD t P <.. 4
5 Figure 2: Men sum of us infeste with thrips per tretment. 1 % Infeste us Avi Steinernem Mtor 83mL Mtor 14mL 1 Numers followe y the sme letter re not signifintly ifferent in LSD t P <.. Figure 3: Men sum of shoots infeste with phis per tretment. 1 % Infeste us Avi Steinernem Mtor 83mL Mtor 14mL 1 Numers followe y the sme letter re not signifintly ifferent in Dunn s MR test t P <..
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